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. 1986 Jan;57(1):55–60.

Different pathways of human T-cell activation revealed by PHA-P and PHA-M.

K O'Flynn, M Russul-Saib, I Ando, D L Wallace, P C Beverley, A W Boylston, D C Linch
PMCID: PMC1453876  PMID: 2417941

Abstract

Antigen-specific T-cell activation is mediated via the CD3-Ti (antigen receptor) complex, and monoclonal antibodies to both CD3 and Ti cause a rapid rise in intracellular Ca2+. This calcium mobilization is not inhibited by monoclonal antibodies to CD2. The rise in calcium mobilization induced by purified PHA (PHA-P) does not occur in a cell line which lacks CD2 expression, and can be blocked in other T cells by anti-CD2 antibodies. A combination of monoclonal antibodies to different epitopes of CD2 causes calcium mobilization and mitogenesis. Reagent grade PHA (PHA-M) induces calcium moblization in cells that lack CD2, and its effects in other T cells cannot be blocked by anti-CD2 antibodies. The effects of PHA-P and PHA-M are thus mediated predominantly through different activation pathways.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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