Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1986 Jan;57(1):37–42.

The importance of the Fc receptors for IgA in the recognition of IgA by mouse liver cells: its comparison with carbohydrate and secretory component receptors.

J Sancho, E Gonzalez, J Egido
PMCID: PMC1453880  PMID: 3002970

Abstract

The numbers of murine hepatocytes and Kupffer cells with receptors for human polymeric (pIgA) or monomeric IgA (mIgA) were determined by rosette formation with immunobeads coated with human pIgA or mIgA. About 63% of hepatocytes were found to express receptors for pIgA. Receptors for mIgA were also found in a significantly lower percentage of hepatocytes (50%). Only about 10% of Kupffer cells had this type of receptor. Blocking studies performed with purified human immunoglobulins suggested that both cells expressed a receptor that recognized the Fc portion of IgA (either pIgA or mIgA). In addition, murine multimeric MOPC-315 IgA strongly inhibited in a dose-dependent fashion the pIgA and mIgA rosette formation by both cells. When cells were incubated in the presence of a rabbit antiserum to secretory component (SC), only pIgA rosette-forming hepatocytes were partially inhibited. No such inhibition was seen when an antiserum to human IgG was used. Both galactose and mannose monosaccharides failed to inhibit the IgA rosette formation by either type of cell. These results show that murine hepatocytes, besides having SC receptors for polymeric IgA, also express receptors for the Fc portion of IgA. The carbohydrate receptors did not seem play any role in the recognition of IgA. The fact that Kupffer cells exclusively present Fc alpha R suggests that monomeric and polymeric IgA, either in form of immune complexes or not, are handled in a different manner by the liver cells.

Full text

PDF
37

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown T. A., Russell M. W., Mestecky J. Hepatobiliary transport of IgA immune complexes: molecular and cellular aspects. J Immunol. 1982 May;128(5):2183–2186. [PubMed] [Google Scholar]
  2. Delacroix D. L., Denef A. M., Acosta G. A., Montgomery P. C., Vaerman J. P. Immunoglobulins in rabbit hepatic bile: selective secretion of IgA and IgM and active plasma-to-bile transfer of polymeric IgA. Scand J Immunol. 1982 Oct;16(4):343–350. doi: 10.1111/j.1365-3083.1982.tb00733.x. [DOI] [PubMed] [Google Scholar]
  3. Delacroix D. L., Furtado-Barreira G., Rahier J., Dive C., Vaerman J. P. Immunohistochemical localization of secretory component in the liver of guinea pigs and dogs versus rats, rabbits, and mice. Scand J Immunol. 1984 May;19(5):425–434. doi: 10.1111/j.1365-3083.1984.tb00951.x. [DOI] [PubMed] [Google Scholar]
  4. Delacroix D. L., Hodgson H. J., McPherson A., Dive C., Vaerman J. P. Selective transport of polymeric immunoglobulin A in bile. Quantitative relationships of monomeric and polymeric immunoglobulin A, immunoglobulin M, and other proteins in serum, bile, and saliva. J Clin Invest. 1982 Aug;70(2):230–241. doi: 10.1172/JCI110610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dooley J. S., Potter B. J., Thomas H. C., Sherlock S. A comparative study of the biliary secretion of human dimeric and monomeric IgA in the rat and in man. Hepatology. 1982 May-Jun;2(3):323–327. doi: 10.1002/hep.1840020306. [DOI] [PubMed] [Google Scholar]
  6. Fanger M. W., Shen L., Pugh J., Bernier G. M. Subpopulations of human peripheral granulocyes and monocytes express receptors for IgA. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3640–3644. doi: 10.1073/pnas.77.6.3640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fisher M. M., Nagy B., Bazin H., Underdown B. J. Biliary transport of IgA: role of secretory component. Proc Natl Acad Sci U S A. 1979 Apr;76(4):2008–2012. doi: 10.1073/pnas.76.4.2008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hall J. G., Gyure L. A., Payne A. W. Comparative aspects of the transport of immunoglobulin A from blood to bile. Immunology. 1980 Dec;41(4):899–902. [PMC free article] [PubMed] [Google Scholar]
  9. Hopf U., Brandtzaeg P., Hütteroth T. H., Meyer zum Büschenfelde K. H. In vivo and in vitro binding of IgA to the plasma membrane of hepatocytes. Scand J Immunol. 1978;8(6):543–549. doi: 10.1111/j.1365-3083.1978.tb00554.x. [DOI] [PubMed] [Google Scholar]
  10. Hsu S. M., Hsu P. L. Demonstration of IgA and secretory component in human hepatocytes. Gut. 1980 Nov;21(11):985–989. doi: 10.1136/gut.21.11.985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Human R. P., Tillotson G. S. Identification of Gardnerella vaginalis with the API 20 Strep system. J Clin Microbiol. 1985 Jun;21(6):985–986. doi: 10.1128/jcm.21.6.985-986.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lawrence D. A., Weigle W. O., Spiegelberg H. L. Immunoglobulins cytophilic for human lymphocytes, monocytes, and neutrophils. J Clin Invest. 1975 Feb;55(2):368–376. doi: 10.1172/JCI107940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lemaître-Coelho I., Altamirano G. A., Barranco-Acosta C., Meykens R., Vaerman J. P. In vivo experiments involving secretory component in the rat hepatic transfer of polymeric IgA from blood into bile. Immunology. 1981 Jun;43(2):261–270. [PMC free article] [PubMed] [Google Scholar]
  14. Nagura H., Smith P. D., Nakane P. K., Brown W. R. IGA in human bile and liver. J Immunol. 1981 Feb;126(2):587–595. [PubMed] [Google Scholar]
  15. Orlans E., Peppard J. V., Payne A. W., Fitzharris B. M., Mullock B. M., Hinton R. H., Hall J. G. Comparative aspects of the hepatobiliary transport of IgA. Ann N Y Acad Sci. 1983 Jun 30;409:411–427. doi: 10.1111/j.1749-6632.1983.tb26886.x. [DOI] [PubMed] [Google Scholar]
  16. Orlans E., Peppard J., Fry J. F., Hinton R. H., Mullock B. M. Secretory component as the receptor for polymeric IgA on rat hepatocytes. J Exp Med. 1979 Dec 1;150(6):1577–1581. doi: 10.1084/jem.150.6.1577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pardo A. G., Lamm M. E., Plaut A. G., Frangione B. Secretory component is convalently bound to a single sub-unit in human secretory IgA. Mol Immunol. 1979 Jul;16(7):477–482. doi: 10.1016/0161-5890(79)90073-7. [DOI] [PubMed] [Google Scholar]
  18. Phillips J. O., Russell M. W., Brown T. A., Mestecky J. Selective hepatobiliary transport of human polymeric IgA in mice. Mol Immunol. 1984 Oct;21(10):907–914. doi: 10.1016/0161-5890(84)90147-0. [DOI] [PubMed] [Google Scholar]
  19. Pricer W. E., Jr, Ashwell G. Subcellular distribution of a mammalian hepatic binding protein specific for asialoglycoproteins. J Biol Chem. 1976 Dec 10;251(23):7539–7544. [PubMed] [Google Scholar]
  20. Putnam F. W., Liu Y. S., Low T. L. Primary structure of a human IgA1 immunoglobulin. IV. Streptococcal IgA1 protease, digestion, Fab and Fc fragments, and the complete amino acid sequence of the alpha 1 heavy chain. J Biol Chem. 1979 Apr 25;254(8):2865–2874. [PubMed] [Google Scholar]
  21. Rifai A., Mannik M. Clearance kinetics and fate of mouse IgA immune complexes prepared with monomeric or dimeric IgA. J Immunol. 1983 Apr;130(4):1826–1832. [PubMed] [Google Scholar]
  22. Robinson E. A., Appella E. Amino acid sequence of a mouse myeloma immunoglobin heavy chain (MOPC 47 A) with a 100-residue deletion. J Biol Chem. 1979 Nov 25;254(22):11418–11430. [PubMed] [Google Scholar]
  23. Sancho J., González E., Egido J. Handling of IgA immune aggregates by liver cells. Contrib Nephrol. 1984;40:93–98. doi: 10.1159/000409734. [DOI] [PubMed] [Google Scholar]
  24. Sancho J., González E., Rivera F., Escanero J. F., Egido J. Hepatic and kidney uptake of soluble monomeric and polymeric IgA aggregates. Immunology. 1984 May;52(1):161–167. [PMC free article] [PubMed] [Google Scholar]
  25. Skogh T. Tissue distribution of intravenously injected dinitrophenylated human serum albumin. Effects of specific IgG and IgA antibodies. Scand J Immunol. 1982 Dec;16(6):465–475. doi: 10.1111/j.1365-3083.1982.tb00747.x. [DOI] [PubMed] [Google Scholar]
  26. Socken D. J., Jeejeebhoy K. N., Bazin H., Underdown B. J. Identification of secretory component as an IgA receptor on rat hepatocytes. J Exp Med. 1979 Dec 1;150(6):1538–1548. doi: 10.1084/jem.150.6.1538. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Stahl P. D., Rodman J. S., Miller M. J., Schlesinger P. H. Evidence for receptor-mediated binding of glycoproteins, glycoconjugates, and lysosomal glycosidases by alveolar macrophages. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1399–1403. doi: 10.1073/pnas.75.3.1399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Stockert R. J., Kressner M. S., Collins J. C., Sternlieb I., Morell A. G. IgA interaction with the asialoglycoprotein receptor. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6229–6231. doi: 10.1073/pnas.79.20.6229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Thornburg R. W., Day J. F., Baynes J. W., Thorpe S. R. Carbohydrate-mediated clearance of immune complexes from the circulation. A role for galactose residues in the hepatic uptake of IgG-antigen complexes. J Biol Chem. 1980 Jul 25;255(14):6820–6825. [PubMed] [Google Scholar]
  30. Tolleshaug H., Brandtzaeg P., Holte K. Quantitative study of the uptake of IgA by isolated rat hepatocytes. Scand J Immunol. 1981;13(1):47–56. doi: 10.1111/j.1365-3083.1981.tb00110.x. [DOI] [PubMed] [Google Scholar]
  31. Toraño A., Tsuzukida Y., Liu Y. S., Putnam F. W. Location and structural significance of the oligosaccharides in human Ig-A1 and IgA2 immunoglobulins. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2301–2305. doi: 10.1073/pnas.74.6.2301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Underdown B. J., De Rose J., Plaut A. Disulfide bonding of secretory component to a single monomer subunit in human secretory IgA. J Immunol. 1977 May;118(5):1816–1821. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES