Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1986 Feb;57(2):231–237.

MRC OX-43: a monoclonal antibody which reacts with all vascular endothelium in the rat except that of brain capillaries.

A P Robinson, T M White, D W Mason
PMCID: PMC1453944  PMID: 2936678

Abstract

A mouse monoclonal antibody, MRC OX-43, has been shown to label vascular endothelium in all tissues of the rat except that of brain capillaries. Using immunoperoxidase staining, the antigen was shown to be expressed on the luminal surface of blood vessels. In addition, this antibody recognized a surface antigen on circulating erythrocytes and some macrophage populations, namely all those in the peritoneal cavity and a subset of alveolar macrophages. The antigen recognized by this antibody was identified on macrophages by metabolic and cell surface labelling followed by immunoprecipitation and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and found to be a surface protein of 90,000 MW.

Full text

PDF
231

Images in this article

235Figure 1
on p.235
235Figure 2
on p.235
235Figure 3
on p.235

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barclay A. N. The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr;42(4):593–600. [PMC free article] [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Bradbury M. W. The structure and function of the blood-brain barrier. Fed Proc. 1984 Feb;43(2):186–190. [PubMed] [Google Scholar]
  4. Brooks C. G., Flannery G. R. Quantitative studies of natural immunity to solid tumours in rats. Persistence of natural immunity throughout reproductive life, and absence of suppressor cells in infant rats. Immunology. 1980 Feb;39(2):187–194. [PMC free article] [PubMed] [Google Scholar]
  5. Clark M. R., Milstein C. Expression of spleen cell immunoglobulin phenotype in hybrids with myeloma cell lines. Somatic Cell Genet. 1981 Nov;7(6):657–666. doi: 10.1007/BF01538755. [DOI] [PubMed] [Google Scholar]
  6. Dallman M. J., Mason D. W., Webb M. The roles of host and donor cells in the rejection of skin allografts by T cell-deprived rats injected with syngeneic T cells. Eur J Immunol. 1982 Jun;12(6):511–518. doi: 10.1002/eji.1830120612. [DOI] [PubMed] [Google Scholar]
  7. Dallman M. J., Thomas M. L., Green J. R. MRC OX-19: a monoclonal antibody that labels rat T lymphocytes and augments in vitro proliferative responses. Eur J Immunol. 1984 Mar;14(3):260–267. doi: 10.1002/eji.1830140311. [DOI] [PubMed] [Google Scholar]
  8. GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
  9. Goud T. J., Schotte C., van Furth R. Identification and characterization of the monoblast in mononuclear phagocyte colonies grown in vitro. J Exp Med. 1975 Nov 1;142(5):1180–1199. doi: 10.1084/jem.142.5.1180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heinemann H. O., Ryan J. W., Ryan U. S. Is the lung a para-endocrine organ? Am J Med. 1977 Oct;63(4):595–603. doi: 10.1016/0002-9343(77)90205-4. [DOI] [PubMed] [Google Scholar]
  11. Hsiung L., Barclay A. N., Brandon M. R., Sim E., Porter R. R. Purification of human C3b inactivator by monoclonal-antibody affinity chromatography. Biochem J. 1982 Apr 1;203(1):293–298. doi: 10.1042/bj2030293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Humphrey J. H., Grennan D. Different macrophage populations distinguished by means of fluorescent polysaccharides. Recognition and properties of marginal-zone macrophages. Eur J Immunol. 1981 Mar;11(3):221–228. doi: 10.1002/eji.1830110311. [DOI] [PubMed] [Google Scholar]
  13. Hunt S. V., Fowler M. H. A repopulation assay for B and T lymphocyte stem cells employing radiation chimaeras. Cell Tissue Kinet. 1981 Jul;14(4):445–464. doi: 10.1111/j.1365-2184.1981.tb00551.x. [DOI] [PubMed] [Google Scholar]
  14. Ishii Y., Matsuura A., Iwaki H., Takami T., Kikuchi K. Two closely related antigens expressed on granulocytes, macrophages and some reticular elements in rat lymphoid tissues: characterization by monoclonal antibodies. Immunology. 1984 Mar;51(3):477–487. [PMC free article] [PubMed] [Google Scholar]
  15. Jefferies W. A., Brandon M. R., Hunt S. V., Williams A. F., Gatter K. C., Mason D. Y. Transferrin receptor on endothelium of brain capillaries. Nature. 1984 Nov 8;312(5990):162–163. doi: 10.1038/312162a0. [DOI] [PubMed] [Google Scholar]
  16. Jefferies W. A., Brandon M. R., Williams A. F., Hunt S. V. Analysis of lymphopoietic stem cells with a monoclonal antibody to the rat transferrin receptor. Immunology. 1985 Feb;54(2):333–341. [PMC free article] [PubMed] [Google Scholar]
  17. Knight S. C., Mertin J., Stackpoole A., Clark J. Induction of immune responses in vivo with small numbers of veiled (dendritic) cells. Proc Natl Acad Sci U S A. 1983 Oct;80(19):6032–6035. doi: 10.1073/pnas.80.19.6032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Knowles D. M., 2nd, Tolidjian B., Marboe C., D'Agati V., Grimes M., Chess L. Monoclonal anti-human monocyte antibodies OKM1 and OKM5 possess distinctive tissue distributions including differential reactivity with vascular endothelium. J Immunol. 1984 May;132(5):2170–2173. [PubMed] [Google Scholar]
  19. Köhler G., Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol. 1976 Jul;6(7):511–519. doi: 10.1002/eji.1830060713. [DOI] [PubMed] [Google Scholar]
  20. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  21. Lampert I. A., Suitters A. J., Chisholm P. M. Expression of Ia antigen on epidermal keratinocytes in graft-versus-host disease. Nature. 1981 Sep 10;293(5828):149–150. doi: 10.1038/293149a0. [DOI] [PubMed] [Google Scholar]
  22. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  23. Ling E. A. Brain macrophages in rats following intravenous labelling of mononuclear leucocytes with colloidal carbon. J Anat. 1978 Jan;125(Pt 1):101–106. [PMC free article] [PubMed] [Google Scholar]
  24. Mason D. W., Dallman M., Barclay A. N. Graft-versus-host disease induces expression of Ia antigen in rat epidermal cells and gut epithelium. Nature. 1981 Sep 10;293(5828):150–151. doi: 10.1038/293150a0. [DOI] [PubMed] [Google Scholar]
  25. Mason D. W. Subsets of T cells in the rat mediating lethal graft versus-host disease. Transplantation. 1981 Sep;32(3):222–226. doi: 10.1097/00007890-198109000-00008. [DOI] [PubMed] [Google Scholar]
  26. Mason D. W., Williams A. F. The kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochem J. 1980 Apr 1;187(1):1–20. doi: 10.1042/bj1870001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. McMaster W. R., Williams A. F. Identification of Ia glycoproteins in rat thymus and purification from rat spleen. Eur J Immunol. 1979 Jun;9(6):426–433. doi: 10.1002/eji.1830090603. [DOI] [PubMed] [Google Scholar]
  28. Pugh C. W., MacPherson G. G., Steer H. W. Characterization of nonlymphoid cells derived from rat peripheral lymph. J Exp Med. 1983 Jun 1;157(6):1758–1779. doi: 10.1084/jem.157.6.1758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Roser B., Ford W. L. Prolonged lymphocytopenia in the rat. the immunological consequences of lymphocyte depletion following injection of 185 W tungsten trioxide into the spleen of lymph nodes. Aust J Exp Biol Med Sci. 1972 Apr;50(2):185–198. [PubMed] [Google Scholar]
  30. Striker G. E., Soderland C., Bowen-Pope D. F., Gown A. M., Schmer G., Johnson A., Luchtel D., Ross R., Striker L. J. Isolation, characterization, and propagation in vitro of human glomerular endothelial cells. J Exp Med. 1984 Jul 1;160(1):323–328. doi: 10.1084/jem.160.1.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Talle M. A., Rao P. E., Westberg E., Allegar N., Makowski M., Mittler R. S., Goldstein G. Patterns of antigenic expression on human monocytes as defined by monoclonal antibodies. Cell Immunol. 1983 May;78(1):83–99. doi: 10.1016/0008-8749(83)90262-9. [DOI] [PubMed] [Google Scholar]
  32. Thomas M. L., Green J. R. Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens. Eur J Immunol. 1983 Oct;13(10):855–858. doi: 10.1002/eji.1830131014. [DOI] [PubMed] [Google Scholar]
  33. Trowbridge I. S., Ralph P., Bevan M. J. Differences in the surface proteins of mouse B and T cells. Proc Natl Acad Sci U S A. 1975 Jan;72(1):157–161. doi: 10.1073/pnas.72.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Welsh K. I., Burgos H., Batchelor J. R. The immune response to allogeneic rat platelets; Ag-B antigens in matrix form lacking Ia. Eur J Immunol. 1977 May;7(5):267–272. doi: 10.1002/eji.1830070505. [DOI] [PubMed] [Google Scholar]
  35. White R. A., Mason D. W., Williams A. F., Galfre G., Milstein C. T-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody. J Exp Med. 1978 Sep 1;148(3):664–673. doi: 10.1084/jem.148.3.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Williams A. F., Galfrè G., Milstein C. Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell. 1977 Nov;12(3):663–673. doi: 10.1016/0092-8674(77)90266-5. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES