Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1986 Feb;57(2):255–259.

The role of monocyte cytotoxic factor in monocyte-mediated lysis of tumour cells.

T Espevik, O Kildahl-Andersen, J Nissen-Meyer
PMCID: PMC1453960  PMID: 3081437

Abstract

The role of a monocyte cytotoxic factor (CF) in monocyte-mediated lysis of leukaemia cells (K562) has been investigated using a polyclonal rabbit antiserum raised against purified CF. The CF antiserum inhibited K562 cell lysis mediated by interferon gamma (IFN-gamma)-activated monocytes. CF antiserum also inhibited monocyte-mediated lysis of antibody-coated K562 cells (AbK562). Preimmune serum at the same concentration as CF antiserum did not affect monocyte-mediated lysis, and the CF antiserum did not inhibit binding between effector and target cells, indicating that inhibition of monocyte-mediated lysis by CF antiserum was not merely a result of toxic components present in the rabbit serum, or a result of a decrease in monocyte-target cell binding. Taken together, the data suggest that CF is involved in monocyte-mediated lysis of uncoated as well as antibody-coated K562 cells.

Full text

PDF
255

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Espevik T. 1,25-Dihydroxyvitamin D3-differentiated human promyelocytic leukaemia cells (HL-60) can kill antibody-coated tumour cells (K562). Scand J Immunol. 1985 Jul;22(1):105–109. doi: 10.1111/j.1365-3083.1985.tb01865.x. [DOI] [PubMed] [Google Scholar]
  2. Espevik T., Hammerstrøm J. Human monocyte-mediated antibody dependent cytotoxicity to K-562 cells: an electron microscopic study. Acta Pathol Microbiol Immunol Scand C. 1983 Jun;91(3):211–219. [PubMed] [Google Scholar]
  3. Espevik T. Human monocyte-mediated lysis of antibody-coated tumor cells: the role of the cytoskeleton in monocytes. J Immunol. 1985 Mar;134(3):2017–2025. [PubMed] [Google Scholar]
  4. Hammerstrøm J. Human monocyte-mediated cytotoxicity to K-562 cells: activation by lymphokines. Scand J Immunol. 1979;10(6):575–584. doi: 10.1111/j.1365-3083.1979.tb01392.x. [DOI] [PubMed] [Google Scholar]
  5. Hammestrøm J. Soluble cytostatic factor(s) released from human monocytes. I. Production and effect on normal and transformed human target cells. Scand J Immunol. 1982 Mar;15(3):311–318. doi: 10.1111/j.1365-3083.1982.tb00654.x. [DOI] [PubMed] [Google Scholar]
  6. Kildahl-Andersen O., Bakke O., Nissen-Meyer J. Human monocyte-released cytotoxic factor. Modulation of cytotoxic activity by inhibitors of cellular DNA, RNA, and protein synthesis. Scand J Immunol. 1985 Sep;22(3):251–258. doi: 10.1111/j.1365-3083.1985.tb01878.x. [DOI] [PubMed] [Google Scholar]
  7. Kildahl-Andersen O., Espevik T., Nissen-Meyer J. IFN-gamma-induced production of monocyte cytotoxic factor. Cell Immunol. 1985 Oct 15;95(2):392–406. doi: 10.1016/0008-8749(85)90326-0. [DOI] [PubMed] [Google Scholar]
  8. Kildahl-Andersen O., Nissen-Meyer J. Effect of lymphokines on the production of cytostatic protein factors from human monocytes. Cell Immunol. 1984 Dec;89(2):365–375. doi: 10.1016/0008-8749(84)90338-1. [DOI] [PubMed] [Google Scholar]
  9. Kildahl-Andersen O., Nissen-Meyer J. Production and characterization of cytostatic protein factors released from human monocytes during exposure to lipopolysaccharide and muramyl dipeptide. Cell Immunol. 1985 Jul;93(2):375–386. doi: 10.1016/0008-8749(85)90142-x. [DOI] [PubMed] [Google Scholar]
  10. Lozzio C. B., Lozzio B. B. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood. 1975 Mar;45(3):321–334. [PubMed] [Google Scholar]
  11. Matthews N. Anti-tumour cytotoxin produced by human monocytes: studies on its mode of action. Br J Cancer. 1983 Sep;48(3):405–410. doi: 10.1038/bjc.1983.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Matthews N. Effect of human monocyte killing of tumour cells of antibody raised against an extracellular monocyte cytotoxin. Immunology. 1983 Feb;48(2):321–327. [PMC free article] [PubMed] [Google Scholar]
  13. Matthews N. Human monocyte killing of tumor cells: contribution of an extracellular cytotoxin. Adv Exp Med Biol. 1982;155:721–729. doi: 10.1007/978-1-4684-4394-3_79. [DOI] [PubMed] [Google Scholar]
  14. Matthews N. Production of an anti-tumour cytotoxin by human monocytes. Immunology. 1981 Sep;44(1):135–142. [PMC free article] [PubMed] [Google Scholar]
  15. Männel D. N., Falk W., Meltzer M. S. Inhibition of nonspecific tumoricidal activity by activated macrophages with antiserum against a soluble cytotoxic factor. Infect Immun. 1981 Jul;33(1):156–164. doi: 10.1128/iai.33.1.156-164.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Männel D. N., Meltzer M. S., Mergenhagen S. E. Generation and characterization of a lipopolysaccharide-induced and serum-derived cytotoxic factor for tumor cells. Infect Immun. 1980 Apr;28(1):204–211. doi: 10.1128/iai.28.1.204-211.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Männel D. N., Moore R. N., Mergenhagen S. E. Macrophages as a source of tumoricidal activity (tumor-necrotizing factor). Infect Immun. 1980 Nov;30(2):523–530. doi: 10.1128/iai.30.2.523-530.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nissen-Meyer J., Hammerstrøm J. Physicochemical characterization of cytostatic factors released from human monocytes. Infect Immun. 1982 Oct;38(1):67–73. doi: 10.1128/iai.38.1.67-73.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nissen-Meyer J., Kildahl-Andersen O. Purification of cytostatic protein factors released from human monocytes. Scand J Immunol. 1984 Oct;20(4):317–325. doi: 10.1111/j.1365-3083.1984.tb01008.x. [DOI] [PubMed] [Google Scholar]
  20. Pennica D., Nedwin G. E., Hayflick J. S., Seeburg P. H., Derynck R., Palladino M. A., Kohr W. J., Aggarwal B. B., Goeddel D. V. Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin. Nature. 1984 Dec 20;312(5996):724–729. doi: 10.1038/312724a0. [DOI] [PubMed] [Google Scholar]
  21. Ruff M. R., Gifford G. E. Purification and physico-chemical characterization of rabbit tumor necrosis factor. J Immunol. 1980 Oct;125(4):1671–1677. [PubMed] [Google Scholar]
  22. Ruff M. R., Gifford G. E. Rabbit tumor necrosis factor: mechanism of action. Infect Immun. 1981 Jan;31(1):380–385. doi: 10.1128/iai.31.1.380-385.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES