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. 1983 Jan;48(1):87–92.

The interchange of derivatives of human B2-microglobulin in HLA alloantigens

P J Ward, A R Sanderson
PMCID: PMC1454005  PMID: 6184315

Abstract

Derivatives of human Beta 2 microglobulin (B2M) have been made by attaching various groups to amino groups in the peptide. In this way 125iodine, dinitrobenzyl, and m-maleimidobenzoyl groups have been introduced. These derivatives of B2M were able to associate with HLA chains and could be displaced again by native B2M. The rate of dissociation of HLA chains and derivatives of B2M is comparable with the intrinsically labelled native dimer. In some cases, particularly with [125I]-B2M, the rate of dissociation appears to be smaller than for native B2M.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appella E., Tanigaki N., Natori T., Pressman D. Partial amino acid sequence of mouse beta2-microglobulin. Biochem Biophys Res Commun. 1976 May 17;70(2):425–430. doi: 10.1016/0006-291x(76)91063-9. [DOI] [PubMed] [Google Scholar]
  2. Becker J. W., Ziffer J. A., Edelman G. M., Cunningham B. A. Crystallographic studies of bovine beta2-microglobulin. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3345–3349. doi: 10.1073/pnas.74.8.3345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berggård I., Bearn A. G. Isolation and properties of a low molecular weight beta-2-globulin occurring in human biological fluids. J Biol Chem. 1968 Aug 10;243(15):4095–4103. [PubMed] [Google Scholar]
  4. Cunningham B. A., Berggard I. Partial amino acid sequence of rabbit beta2-microglobulin. Science. 1975 Mar 21;187(4181):1079–1080. doi: 10.1126/science.46618. [DOI] [PubMed] [Google Scholar]
  5. EISEN H. N. PREPARATION OF PURIFIED ANTI-2,4-DINITROPHENYL ANTIBODIES. Methods Med Res. 1964;10:94–102. [PubMed] [Google Scholar]
  6. Goodfellow P. N., Jones E. A., Van Heyningen V., Solomon E., Bobrow M., Miggiano V., Bodmer W. F. The beta2-microglobulin gene is on chromosome 15 and not in the HL-A region. Nature. 1975 Mar 20;254(5497):267–269. doi: 10.1038/254267a0. [DOI] [PubMed] [Google Scholar]
  7. Hester R. B., Kubo R. T., Grey H. M. Studies on the cytophilic properties of human beta2-microglobulin. II. The role of histocompatibility antigens. Scand J Immunol. 1979;9(2):125–134. doi: 10.1111/j.1365-3083.1979.tb02714.x. [DOI] [PubMed] [Google Scholar]
  8. Hyafil F., Strominger J. L. Dissociation and exchange of the beta 2-microglobulin subunit of HLA-A and HLA-B antigens. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5834–5838. doi: 10.1073/pnas.76.11.5834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kitagawa T., Aikawa T. Enzyme coupled immunoassay of insulin using a novel coupling reagent. J Biochem. 1976 Jan;79(1):233–236. doi: 10.1093/oxfordjournals.jbchem.a131053. [DOI] [PubMed] [Google Scholar]
  10. Nakamuro K., Tanigaki N., Pressman D. Isolation of HL-A 33,000-dalton fragments carrying high HL-A alloantigenic activity. Transplantation. 1975 May;19(5):431–437. [PubMed] [Google Scholar]
  11. Ploegh H. L., Cannon L. E., Strominger J. L. Cell-free translation of the mRNAs for the heavy and light chains of HLA-A and HLA-B antigens. Proc Natl Acad Sci U S A. 1979 May;76(5):2273–2277. doi: 10.1073/pnas.76.5.2273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Robinson P. J., Graf L., Sege K. Two allelic forms of mouse beta 2-microglobulin. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1167–1170. doi: 10.1073/pnas.78.2.1167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sanderson A. R. HL-A substances from human spleens. Nature. 1968 Oct 12;220(5163):192–195. doi: 10.1038/220192a0. [DOI] [PubMed] [Google Scholar]
  14. Schmidt W., Festenstein H., Ward P. J., Sanderson A. R. Interspecies exchange of beta 2-microglobulin and associated MHC and differentiation antigens. Immunogenetics. 1981;13(6):483–491. doi: 10.1007/BF00343716. [DOI] [PubMed] [Google Scholar]
  15. Sedlak J., Lindsay R. H. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 1968 Oct 24;25(1):192–205. doi: 10.1016/0003-2697(68)90092-4. [DOI] [PubMed] [Google Scholar]
  16. Smithies O., Poulik M. D. Dog homologue of human 2 -microglobulin. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2914–2917. doi: 10.1073/pnas.69.10.2914. [DOI] [PMC free article] [PubMed] [Google Scholar]

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