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. 1983 Mar;48(3):551–559.

T-cell hybridoma co-expressing Fc receptors for different isotypes. I. Reciprocal regulation of Fc alpha R and Fc gamma R expression by IgA and interferon.

J Yodoi, M Adachi, K Teshigawara, T Masuda, W H Fridman
PMCID: PMC1454037  PMID: 6219065

Abstract

To clarify the co-expression phenomenon of T-cell Fc receptors (FcR) specific for different isotypes on the clonal level, a murine hybridoma clone T2D4 was studied. T2D4 cells originally reported to bear FcR for IgG (Fc gamma R) and to release a Fc gamma R-related T-cell factor binding to IgG (immunoglobulin binding factor; IBF) proved to have also the receptor for IgA. The binding of IgA was detected by rosette formation with trinitrophenylated ox red blood cells (TNP-ORBC) after preincubation of T2D4 cells with MOPC 315 IgA having anti-TNP activity, or directly with TNP-ORBC sensitized with MOPC 315 IgA. While the binding of MOPC 315 IgA was competed for by IgA but not by IgG2A nor IgG2B, IgA failed to inhibit the rosette formation of the cells with ORBC sensitized with rabbit IgG antibody (EA ox gamma), proving that T2D4 cells express FcR specific for IgA (Fc alpha R) in addition to Fc gamma R. Co-expression of both receptors on the same cell surface was demonstrated by a double rosette technique using TNP-quail red blood cells (TNP-QRBC) and EAox gamma. Fc alpha R activity of the cells was completely abrogated by 15 min. incubation with 0.1 mg/ml trypsin, whereas Fc gamma R was resistant even to 1 mg/ml trypsin. The expression of Fc alpha R was augmented (up-regulation) by IgA at the concentration above 300 micrograms/ml and inhibited (down-regulation) by 1000 u./ml of murine beta-interferon (beta-IFN). Conversely, the expression of Fc gamma R was down-regulated by IgA and up-regulated by alpha-IFN. Thus, Fc gamma R and Fc alpha R are co-expressed and reciprocally regulated on these cell lines. The possible co-production of IBF and the Fc alpha R-related binding factor specific for IgA is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chen S. S., Bohn J. W., Liu F. T., Katz D. H. Murine lymphocytes expressing Fc receptors for IgE (FcR epsilon). I. Conditions for inducing FcR epsilon + lymphocytes and inhibition of the inductive events by suppressive factor of allergy (SFA). J Immunol. 1981 Jul;127(1):166–173. [PubMed] [Google Scholar]
  2. Endoh M., Sakai H., Nomoto Y., Tomino Y., Kaneshige H. IgA-specific helper activity of T alpha cells in human peripheral blood. J Immunol. 1981 Dec;127(6):2612–2613. [PubMed] [Google Scholar]
  3. Fridman W. H., Fradelizi D., Guimezanes A., Plater C., Leclerc J. C. The role of the Fc receptor (FcR) of thymus-derived lymphocytes. II. Presence of FcR on suppressor cells and direct involvement in suppression. Eur J Immunol. 1977 Aug;7(8):549–554. doi: 10.1002/eji.1830070811. [DOI] [PubMed] [Google Scholar]
  4. Fridman W. H., Gresser I., Bandu M. T., Aguet M., Neauport-Sautes C. Interferon enhances the expression of Fc gamma receptors. J Immunol. 1980 May;124(5):2436–2441. [PubMed] [Google Scholar]
  5. Fritsche R., Spiegelberg H. L. Fc receptors for IgE on normal rat lymphocytes. J Immunol. 1978 Aug;121(2):471–478. [PubMed] [Google Scholar]
  6. Gisler R. H., Fridman W. H. Suppression of in vitro antibody synthesis by immunoglobulin-binding factor. J Exp Med. 1975 Aug 1;142(2):507–517. doi: 10.1084/jem.142.2.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gresser I., Guy-Grand D., Maury C., Maunoury M. T. Interferon induces peripheral lymphadenopathy in mice. J Immunol. 1981 Oct;127(4):1569–1575. [PubMed] [Google Scholar]
  8. Guimezanes A., Fridman W. H., Gisler R. H., Kourilsky F. M. Reversible binding of a T cell factor on IgG-coated sepharose beads. Eur J Immunol. 1976 Jan;6(1):69–72. doi: 10.1002/eji.1830060115. [DOI] [PubMed] [Google Scholar]
  9. Gupta S., Platsoucas C. D., Good R. A. Receptors for IgA on a subpopulation of human B lymphocytes. Proc Natl Acad Sci U S A. 1979 Aug;76(8):4025–4028. doi: 10.1073/pnas.76.8.4025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Haegert D. G. Neuraminidase- and trypsin-induced exposure to membrane receptors for IgG and IgM molecules on human peripheral blood lymphocytes. Clin Exp Immunol. 1977 Sep;29(3):457–463. [PMC free article] [PubMed] [Google Scholar]
  11. Harwell L., Skidmore B., Marrack P., Kappler J. Concanavalin A-inducible, interleukin-2-producing T cell hybridoma. J Exp Med. 1980 Oct 1;152(4):893–904. doi: 10.1084/jem.152.4.893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hoover R. G., Lynch R. G. Lymphocyte surface membrane immunoglobulin in myeloma. II. T cells with IgA-Fc receptors are markedly increased in mice with IgA plasmacytomas. J Immunol. 1980 Sep;125(3):1280–1288. [PubMed] [Google Scholar]
  13. Hämmerling G. J. T lymphocyte tissue culture lines produced by cell hybridization. Eur J Immunol. 1977 Oct;7(10):743–746. doi: 10.1002/eji.1830071018. [DOI] [PubMed] [Google Scholar]
  14. Ishizaka K., Suemura M., Yodoi J., Hirashima M. Regulation of IgE response by IgE binding factors. Fed Proc. 1981 Jun;40(8):2162–2166. [PubMed] [Google Scholar]
  15. Itoh K., Inoue M., Kataoka S., Kumagai K. Differential effect of interferon expression of IgG- and IgM-Fc receptors on human lymphocytes. J Immunol. 1980 Jun;124(6):2589–2595. [PubMed] [Google Scholar]
  16. Moretta L., Webb S. R., Grossi C. E., Lydyard P. M., Cooper M. D. Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J Exp Med. 1977 Jul 1;146(1):184–200. doi: 10.1084/jem.146.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nabel G., Greenberger J. S., Sakakeeny M. A., Cantor H. Multiple biologic activities of a cloned inducer T-cell population. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1157–1161. doi: 10.1073/pnas.78.2.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Neauport-Sautes C., Rabourdin-Combe C., Fridman W. H. T-cell hybrids bear Fcgamma receptors and secrete suppressor immunoglobulin binding factor. Nature. 1979 Feb 22;277(5698):656–659. doi: 10.1038/277656a0. [DOI] [PubMed] [Google Scholar]
  19. Pichler W. J., Lum L., Broder S. Fc-receptors on human T lymphocytes. I. Transition of Tgamma to Tmu cells. J Immunol. 1978 Oct;121(4):1540–1548. [PubMed] [Google Scholar]
  20. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  21. Strober W., Hague N. E., Lum L. G., Henkart P. A. IgA-Fc receptors on mouse lymphoid cells. J Immunol. 1978 Dec;121(6):2440–2445. [PubMed] [Google Scholar]
  22. Unkeless J. C., Eisen H. N. Binding of monomeric immunoglobulins to Fc receptors of mouse macrophages. J Exp Med. 1975 Dec 1;142(6):1520–1533. doi: 10.1084/jem.142.6.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Watanabe T., Kimoto M., Maruyama S., Kishimoto T., Yamamura Y. Regulation of antibody response in different immunoglobulin classes. V. Establishment of T hybrid cell line secreting IgE class-specific suppressor factor. J Immunol. 1978 Nov;121(5):2113–2117. [PubMed] [Google Scholar]
  24. Yodoi J., Adachi M., Masuda T. Induction of FcR alpha on murine lymphocytes by IgA in vitro. J Immunol. 1982 Feb;128(2):888–894. [PubMed] [Google Scholar]
  25. Yodoi J., Ishizaka K. Lymphocytes bearing Fc receptors for IgE. I. Presence of human and rat T lymphocytes with Fc epsilon receptors. J Immunol. 1979 Jun;122(6):2577–2583. [PubMed] [Google Scholar]
  26. Yodoi J., Ishizaka K. Lymphocytes bearing receptors for IgE. III. Transition of Fc gamma R(+) cells to Fc epsilon R(4) cells by IgE. J Immunol. 1979 Nov;123(5):2004–2010. [PubMed] [Google Scholar]
  27. Yodoi J., Ishizaka T., Ishizaka K. Lymphocytes bearing Fc receptors for IgE. II. Induction of Fcepsilon-receptor bearing rat lymphocytes by IgE. J Immunol. 1979 Jul;123(1):455–462. [PubMed] [Google Scholar]

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