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. 1983 Mar;48(3):597–603.

Subclass specificities of rabbit antibodies to human antidextran.

I M Outschoorn
PMCID: PMC1454045  PMID: 6186599

Abstract

Rabbits were immunized with purified antidextran containing IgG2. Half of the animals were treated with commercial human IgG intravenously to induce tolerance. The antisera were absorbed and/or eluted from IgG-Sepharose columns and the antibodies tested with a panel of at least eight human myeloma proteins of the four IgG subclasses, both kappa and lambda types. All animals produced mainly anti-IgG2 or IgG4, sometimes intechanged between these maxima or between kappa and lambda types over a series of bleedings. Absorption with IgG2 and/or IgG4 myeloma proteins resulted in sera or antibody preparations specific for the IgG1 or IgG3 subclasses, and the amount of those antibodies also varied between bleedings from the same animal. It may be concluded that anti-IgG2 and anti-IgG4 syntheses are related in a complementary manner or are equally likely to be produced in a response to IgG2; while IgG1 and IgG3 are also interrelated, but with an inverse relationship to IgG2 and IgG4. Alternatively anti-IgG2 and anti-IgG4 antibodies could be highly cross-reactive, and so could anti-IgG1 and anti-IgG3 antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLLEN J. C., KUNKEL H. G., KABAT E. A. STUDIES ON HUMAN ANTIBODIES. II. DISTRIBUTION OF GENETIC FACTORS. J Exp Med. 1964 Mar 1;119:453–465. doi: 10.1084/jem.119.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cuatrecasas P. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads. J Biol Chem. 1970 Jun;245(12):3059–3065. [PubMed] [Google Scholar]
  3. Dorner M. M., Yount W. J., Kabat E. A. Studies on human antibodies. VII. Acrylamide gel electrophoresis of purified human antibodies and myeloma proteins, their heavy and light chains. J Immunol. 1969 Feb;102(2):273–282. [PubMed] [Google Scholar]
  4. GREY H. M., KUNKEL H. G. H CHAIN SUBGROUPS OF MYELOMA PROTEINS AND NORMAL 7S GAMMA-GLOBULIN. J Exp Med. 1964 Aug 1;120:253–266. doi: 10.1084/jem.120.2.253. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goosen P. C., Van Beekhuizen S., Droogh C., De Lange G. Preparation of antibodies against subclasses of human IgG. J Immunol Methods. 1981;40(3):339–344. doi: 10.1016/0022-1759(81)90365-3. [DOI] [PubMed] [Google Scholar]
  6. Harisdangkul V., Kabat E. A. Studies on human antibodies. IX. Interaction of 1-(m-nitrophenyl)-flavazoles of isomaltose oligosaccharides with purified antidextrans: quantitative hapten-inhibition and fluorescence quenching studies. J Immunol. 1972 May;108(5):1232–1243. [PubMed] [Google Scholar]
  7. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  8. Oudin J., Cazenave P. A. Similar idiotypic specificities in immunoglobulin fractions with different antibody functions or even without detectable antibody function. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2616–2620. doi: 10.1073/pnas.68.10.2616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Outschoorn I. M., Ashwell G., Gruezo F., Kabat E. A. Immunochemical studies on dextrans. 8. Specificity and cross-reactivity with dextrans of the antibodies formed in rabbits to isomaltohexaonic acid coupled to bovine serum albumin. J Immunol. 1974 Sep;113(3):896–903. [PubMed] [Google Scholar]
  10. Outschoorn I. M. Idiotypic specificities and cross-reactivities of rabbit antibodies to human antidextran. Immunology. 1979 Oct;38(2):225–234. [PMC free article] [PubMed] [Google Scholar]
  11. Oxelius V. A., Berkel A. I., Hanson L. A. IgG2 deficiency in ataxia-telangiectasia. N Engl J Med. 1982 Mar 4;306(9):515–517. doi: 10.1056/NEJM198203043060905. [DOI] [PubMed] [Google Scholar]
  12. SCHIFFMAN G., KABAT E. A., THOMPSON W. IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS. XXX. CLEAVAGE OF A, B, AND H BLOOD-GROUP SUBSTANCES BY ALKALI. Biochemistry. 1964 Jan;3:113–120. doi: 10.1021/bi00889a018. [DOI] [PubMed] [Google Scholar]
  13. SCHLOSSMAN S. F., KABAT E. A. Specific fractionation of a population of antidextran molecules with combining sites of various sizes. J Exp Med. 1962 Oct 1;116:535–552. doi: 10.1084/jem.116.4.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sharon J., D'Hoostelaere L., Potter M., Kabat E. A., Morrison S. L. A cross-reactive idiotype, QUPC52 IdX, present on most but not all anti-alpha (1 replaced by 6) dextran-specific IgM and IgA hybridoma antibodies with combining sites of different sizes. J Immunol. 1982 Jan;128(1):498–500. [PubMed] [Google Scholar]
  15. Sharon J., Kabat E. A., Morrison S. L. Association constants of hybridoma antibodies specific for alpha (1 leads to 6) linked dextran determined by affinity electrophoresis. Mol Immunol. 1982 Mar;19(3):389–397. doi: 10.1016/0161-5890(82)90204-8. [DOI] [PubMed] [Google Scholar]
  16. Sharon J., Kabat E. A., Morrison S. L. Immunochemical characterization of binding sites of hybridoma antibodies specific for alpha (1 leads to 6) linked dextran. Mol Immunol. 1982 Mar;19(3):375–388. doi: 10.1016/0161-5890(82)90203-6. [DOI] [PubMed] [Google Scholar]
  17. Sharon J., Kabat E. A., Morrison S. L. Studies on mouse hybridomas secreting IgM or IgA antibodies to alpha(1 to 6)-linked dextran. Mol Immunol. 1981 Sep;18(9):831–846. doi: 10.1016/0161-5890(81)90005-5. [DOI] [PubMed] [Google Scholar]
  18. Skvaril F., Probst M., Audran R., Steinbuch M. Distribution of IgG subclasses in commercial and some experimental gamma-globulin preparations. Vox Sang. 1977;32(6):335–338. doi: 10.1111/j.1423-0410.1977.tb00655.x. [DOI] [PubMed] [Google Scholar]
  19. Spiegelberg H. L., Weigle W. O. The production of antisera to human gammaGlobulin subclasses in rabbits using immunological unresponsiveness. J Immunol. 1968 Aug;101(2):377–380. [PubMed] [Google Scholar]
  20. TERRY W. D., FAHEY J. L. SUBCLASSES OF HUMAN GAMMA-2-GLOBULIN BASED ON DIFFERENCES IN THE HEAVY POLYPEPTIDE CHAINS. Science. 1964 Oct 16;146(3642):400–401. doi: 10.1126/science.146.3642.400. [DOI] [PubMed] [Google Scholar]
  21. Yount W. J., Dorner M. M., Kunkel H. G., Kabat E. A. Studies on human antibodies. VI. Selective variations in subgroup composition and genetic markers. J Exp Med. 1968 Mar 1;127(3):633–646. doi: 10.1084/jem.127.3.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. van der Giessen M., de Lange B., van der Lee B. The production of precipitating antiglobulin reagents specific for the subclasses of human IgG. Immunology. 1974 Oct;27(4):655–663. [PMC free article] [PubMed] [Google Scholar]

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