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. 1983 Apr;48(4):763–769.

Preferential expression of IgG1 antibodies specific for the L2C leukaemia IgM idiotypic determinants in tumour-protected strain 2 guinea-pigs

M J Ricardo Jr, D T Grimm
PMCID: PMC1454055  PMID: 6187671

Abstract

Immunization of strain 2 guinea-pigs with 107 syngeneic Ia+ L2C leukaemia cells in adjuvant leads to L2C tumour protection. After subsequent challenges with L2C tumour cells, the sera of twelve out of seventy protected guinea-pigs had detectable L2C reactivity as determined by a [125I]-protein A binding assay. The antibodies bound equally well to Ia+ and Ia- L2C tumour cells, but did not bind to L1 and L10 guinea-pig hepatocarcinoma cells or normal guinea-pig B and T lymphocytes. The binding was blocked appreciably by F(ab')2 reagents specific for the L2C IgM idiotype but not by those specific for Ia or B.1 alloantigens or β2 microglobulin. These results lead to provisional identification of anti-idiotype among the syngeneic antibody population. After ion-exchange chromatography, the L2C reactivity in eleven of the twelve immune sera analysed was exclusively in the IgG1 fraction. The syngeneic anti-idiotypic antibodies precipitated only IgM molecules from the NP-40 extracts of L2C tumour cells and were dissociated from the L2C leukaemia cells more readily than the xenogeneic anti-idiotypic antibodies at pH 6.5 and 6.0. These results suggest that the L2C IgM idiotype may function as a tumour-associated antigen or is near the antigenic complex recognized by the low affinity L2C antibodies. The preferential expression of IgG1 antibodies suggests that humoral immunity effects a minimum level of protection because this isotype, in the guinea-pig, has a restricted capacity to mediate tumour rejection by secondary immune mechanisms.

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Selected References

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  1. BLOCH K. J., KOURILSKY F. M., OVARY Z., BENACERRAF B. Properties of guinea pig 7S antibodies. III. Identification of antibodies involved in complement fixation and hemolysis. J Exp Med. 1963 Jun 1;117:965–981. doi: 10.1084/jem.117.6.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CONGDON C. C., LORENZ E. Leukemia in guinea-pigs. Am J Pathol. 1954 Mar-Apr;30(2):337–359. [PMC free article] [PubMed] [Google Scholar]
  3. Eichmann K. Expression and function of idiotypes of lymphocytes. Adv Immunol. 1978;26:195–254. doi: 10.1016/s0065-2776(08)60231-x. [DOI] [PubMed] [Google Scholar]
  4. Forni G., Shevach E. M., Green I. Mutant lines of guinea pig L2C leukemia. I. Deletion of Ia alloantigens is associated with a loss in immunogenicity of tumor-associated transplantation antigens. J Exp Med. 1976 May 1;143(5):1067–1081. doi: 10.1084/jem.143.5.1067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hough D. W., Chapple J. C., Stevenson F. K., Stevenson G. T. Further studies of immunoglobulin synthesis by guinea pig leukaemic lymphocytes. Immunology. 1978 May;34(5):889–899. [PMC free article] [PubMed] [Google Scholar]
  6. Hu C. P., Schwartz B. D., Green I. Identification of a tumor-associated antigen of the guinea pig L2C leukemia by using syngeneic antisera. J Immunol. 1978 Feb;120(2):597–601. [PubMed] [Google Scholar]
  7. Klein G., Klein E. Rejectability of virus-induced tumors and nonrejectability of spontaneous tumors: a lesson in contrasts. Transplant Proc. 1977 Mar;9(1):1095–1104. [PubMed] [Google Scholar]
  8. Lewis M. G., Philips T. M., Rowden G. Beneficial and detrimental effects of humoral immunity in malignancy. Pathobiol Annu. 1978;8:217–239. [PubMed] [Google Scholar]
  9. Ohlander C., Larsson A., Perlmann P. Specificity of Fc-receptors on lymphocytes and monocytes for guinea-pig IgG1 and IgG2: phagocytosis of erythrocytes. Scand J Immunol. 1978 Apr;7(4):285–296. doi: 10.1111/j.1365-3083.1978.tb00456.x. [DOI] [PubMed] [Google Scholar]
  10. Ricardo M. J., Jr Heterogeneity of Fc-receptors on guinea pig T lymphocytes. J Immunol. 1980 Nov;125(5):2009–2016. [PubMed] [Google Scholar]
  11. Ricardo M. J., Jr Isotype specificity of Fc gamma-receptors on guinea-pig T lymphocytes and their modulation by homologous immune complexes. Immunology. 1981 Mar;42(3):459–467. [PMC free article] [PubMed] [Google Scholar]
  12. Ricardo M. J., Jr, Trouy R. L., Grimm D. T. Effect of pH on the binding between guinea pig IgG isotypes and protein A: evidence for intra-isotypic binding heterogeneity. J Immunol. 1981 Sep;127(3):946–951. [PubMed] [Google Scholar]
  13. Schwartz B. D., McMillan M., Shevach E., Hahn Y., Rose S. M., Hood L. Partial N-terminal amino acid sequences of guinea pig classic histocompatibility antigens. J Immunol. 1980 Sep;125(3):1055–1059. [PubMed] [Google Scholar]
  14. Shevach E. M., Paul W. E., Green I. Histocompatibility-linked immune response gene function in guinea pigs. Specific inhibition of antigen-induced lymphocyte proliferation by alloantisera. J Exp Med. 1972 Nov 1;136(5):1207–1221. doi: 10.1084/jem.136.5.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shin H. S., Johnson R. J., Pasternack G. R., Economou J. S. Mechanisms of tumor immunity: the role of antibody and nonimmune effectors. Prog Allergy. 1978;25:163–210. [PubMed] [Google Scholar]
  16. Stevenson F. K., Elliott E. V., Stevenson G. T. Some effects on leukaemic B lymphocytes of antibodies to defined regions of their surface immunoglobulin. Immunology. 1977 Apr;32(4):549–557. [PMC free article] [PubMed] [Google Scholar]
  17. Stevenson F. K., Morris D., Stevenson G. T. Immunoglobulin produced by guinea-pig leukaemic B lymphocytes: its source and use as a monitor of tumour load. Immunology. 1980 Oct;41(2):313–321. [PMC free article] [PubMed] [Google Scholar]
  18. Stevenson G. T., Stevenson F. K. Antibody to a molecularly-defined antigen confined to a tumour cell surface. Nature. 1975 Apr 24;254(5502):714–716. doi: 10.1038/254714a0. [DOI] [PubMed] [Google Scholar]

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