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. 1983 Apr;48(4):703–711.

Antibody response to dinitrophenyl hapten by foetal, neonatal and young pigs.

D B Symons, C A Clarkson, R M Binns
PMCID: PMC1454056  PMID: 6832803

Abstract

The immunological competence of foetal (85 days of gestation), neonatal and older pigs has been assessed by their ability to make heterogeneous IgG antibody to dinitrophenyl (DNP) hapten. Antibody heterogeneity was resolved by isoelectric focusing (IEF) followed by overlay of labelled antigen. Choice of protein carrier (bovine serum albumin, keyhole limpet haemocyanin or ovalbumin) had little effect on anti-DNP responses. Pigs of all three ages were capable of producing isoelectrically heterogeneous antibody which did not become more diverse, either with repeated immunization or with increasing age. Foetal pigs did not all respond equally strongly as assessed by IEF, but those with clearly heterogeneous antibody showed IEF patterns similar to 'adult' type anti-DNP. This demonstrates that by 85 days of gestation the pig foetus is capable of a mature response to an antigen which is T-dependent in rodents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binns R. M. Bone marrow and lymphoid cell injection of the pig foetus resulting in transplantation tolerance or immunity, and immunoglobulin production. Nature. 1967 Apr 8;214(5084):179–180. doi: 10.1038/214179a0. [DOI] [PubMed] [Google Scholar]
  2. Binns R. M., Symons D. B. The ontogeny of lymphocyte immunoglobin determinants and their relationship to antibody production in the fetal pig. Res Vet Sci. 1974 Mar;16(2):260–262. [PubMed] [Google Scholar]
  3. Franek F., Olsovská Z., Simek L. Nonprecipitating pig anti-dinitrophenyl antibody: factors influencing the conversion into precipitating antibody. Eur J Immunol. 1979 Sep;9(9):696–701. doi: 10.1002/eji.1830090907. [DOI] [PubMed] [Google Scholar]
  4. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  5. Katz D. H., Paul W. E., Goidl E. A., Benacerraf B. Carrier function in anti-hapten immune responses. I. Enhancement of primary and secondary anti-hapten antibody responses by carrier preimmunization. J Exp Med. 1970 Aug 1;132(2):261–282. doi: 10.1084/jem.132.2.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Keck K., Grossberg A. L., Pressmann D. Specific characterization of isoelectrofocused immunoglobulins in polyacrylamide gel by reaction with 125 I-labeled protein antigens or antibodies. Eur J Immunol. 1973 Feb;3(2):99–102. doi: 10.1002/eji.1830030209. [DOI] [PubMed] [Google Scholar]
  7. Kim Y. B., Bradley S. G., Watson D. W. Ontogeny of the immune response. I. Development of immunoglobulins in germfree and conventional colostrum-deprived piglets. J Immunol. 1966 Jul;97(1):52–63. [PubMed] [Google Scholar]
  8. Kipps T. J., Benacerraf B., Dorf M. E. [Analysis of the restricted dinitrophenyl (DNP) antibody response to the DNP conjugate of poly-(L-Glu56, L-LYS35, L-PHE9)]. J Immunol. 1978 Jan;120(1):46–52. [PubMed] [Google Scholar]
  9. Klaus G. G., Phillips J. M., Humphrey J. H., Dresser D. W., Cross A. M. The immunological properties of haptens coupled to thymus-independent carrier molecules. IV. The IgG response to dinitrophenylated Ficoll. Eur J Immunol. 1976 Jun;6(6):429–433. doi: 10.1002/eji.1830060610. [DOI] [PubMed] [Google Scholar]
  10. Mitchison N. A. The carrier effect in the secondary response to hapten-protein conjugates. I. Measurement of the effect with transferred cells and objections to the local environment hypothesis. Eur J Immunol. 1971 Jan;1(1):10–17. doi: 10.1002/eji.1830010103. [DOI] [PubMed] [Google Scholar]
  11. Montgomery P. C., Williamson A. R. Molecular restriction of anti-hapten antibody elicited in neonatal rabbits. Nature. 1970 Dec 26;228(5278):1306–1308. doi: 10.1038/2281306a0. [DOI] [PubMed] [Google Scholar]
  12. Montgomery P. C., Williamson A. R. Molecular restriction of anti-hapten antibody elicited in neonatal rabbits: antibody production in littermates. J Immunol. 1972 Nov;109(5):1036–1045. [PubMed] [Google Scholar]
  13. North J. R., Feinstein A. Analysis of anti-hapten foci in repopulated mouse spleens. Eur J Immunol. 1973 Dec;3(12):784–789. doi: 10.1002/eji.1830031209. [DOI] [PubMed] [Google Scholar]
  14. Phillips J. M., Dresser D. W. Isoelectric spectra of different classes of anti-erythrocyte antibodies. Eur J Immunol. 1973 Aug;3(8):524–527. doi: 10.1002/eji.1830030816. [DOI] [PubMed] [Google Scholar]
  15. Phillips J. M., Waldmann H. Monogamous T helper cell. Nature. 1977 Aug 18;268(5621):641–642. doi: 10.1038/268641a0. [DOI] [PubMed] [Google Scholar]
  16. Rajewsky K., Schirrmacher V., Nase S., Jerne N. K. The requirement of more than one antigenic determinant for immunogenicity. J Exp Med. 1969 Jun 1;129(6):1131–1143. doi: 10.1084/jem.129.6.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sullman S. F., Feinstein A. The relative distribution of foci forming antibodies to two different haptens in repopulated mouse spleens. Eur J Immunol. 1977 Jul;7(7):421–425. doi: 10.1002/eji.1830070704. [DOI] [PubMed] [Google Scholar]
  18. Symons D. B., Clarkson C. A. Isoelectric heterogeneity of immunoglobulin secreted by LPS-activated neonatal and adult pig lymphocytes in vitro. Immunology. 1980 Dec;41(4):875–881. [PMC free article] [PubMed] [Google Scholar]
  19. Williamson A. R. Antibody isoelectric spectra. Analysis of the heterogeneity of antibody molecules in serum by isoelectric focusing in gel and specific detection with hapten. Eur J Immunol. 1971 Nov;1(5):390–394. doi: 10.1002/eji.1830010517. [DOI] [PubMed] [Google Scholar]

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