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. 1983 May;49(1):169–174.

Human peripheral blood monocyte-derived macrophages produce haemolytically active C3 in vitro.

R C Strunk, K S Kunke, P C Giclas
PMCID: PMC1454087  PMID: 6840804

Abstract

The third component of complement (C3) synthesized by human monocyte-derived macrophages has been shown to have the same size and sub-unit structure as serum C3, but haemolytic activity has not been demonstrated. Human monocyte-derived macrophages were cultured from days 4 to 7 in medium without serum, and the conditioned medium was dialysed to remove inhibitors of the C3 assay and concentrated to enhance detection of low amounts of C3. Using these techniques C3 activity was detected routinely. The amount of C3 was 3.4 x 10(7) effective C3 molecules/ml of concentrated tissue culture medium (range 1.0-7.5 x 10(7)), and the number of C3 molecules synthesized by each cell was 4.4 x 10(5), assuming that each cell synthesized C3. The specific activity of the C3 synthesized by the monocytes was the same as the specific activity of C3 that had been purified from serum and then incubated with the cells and processed in the same manner as the monocyte media. Synthesis as the basis for the presence of the C3 activity in the medium was indicated by an inhibition of production of the C3 activity of 66 +/- 16% by cycloheximide, 2 micrograms/ml. Thus, human blood monocytes that migrate into areas of inflammation can mature into cells capable of producing C3 which can participate in the complement sequence and thus potentiate inflammation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beatty D. W., Davis A. E., 3rd, Cole F. S., Einstein L. P., Colten H. R. Biosynthesis of complement by human monocytes. Clin Immunol Immunopathol. 1981 Mar;18(3):334–343. doi: 10.1016/0090-1229(81)90126-4. [DOI] [PubMed] [Google Scholar]
  2. Colten H. R., Alper C. A. Hemolytic efficiencies of genetic variants of human C3. J Immunol. 1972 May;108(5):1184–1187. [PubMed] [Google Scholar]
  3. Cooper N. R., Müller-Eberhard H. J. The reaction mechanism of human C5 in immune hemolysis. J Exp Med. 1970 Oct 1;132(4):775–793. doi: 10.1084/jem.132.4.775. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Einstein L. P., Hansen P. J., Ballow M., Davis A. E., 3rd, Davis J. S., 4th, Alper C. A., Rosen F. S., Colten H. R. Biosynthesis of the third component of complement (C3) in vitro by monocytes from both normal and homozygous C3-deficient humans. J Clin Invest. 1977 Nov;60(5):963–969. doi: 10.1172/JCI108876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. McCarthy K., Musson R. A., Henson P. M. Protein synthesis-dependent and protein synthesis-independent secretion of lysosomal hydrolases from rabbit and human macrophages. J Reticuloendothel Soc. 1982 Feb;31(2):131–144. [PubMed] [Google Scholar]
  6. Müllerèberhard H. J., Dalmasso A. P., Calcott M. A. The reaction mechanism of beta-1C-globulin (C'3) in immune hemolysis. J Exp Med. 1966 Jan 1;123(1):33–54. doi: 10.1084/jem.123.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Opferkuch W., Rapp H. J., Colten H. R., Borsos T. Immune hemolysis and the functional properties of the second (C2) and fourth (C4) components of complement. II. Clustering of effective C42 complexes at individual hemolytic sites. J Immunol. 1971 Feb;106(2):407–413. [PubMed] [Google Scholar]
  8. Pangburn M. K., Schreiber R. D., Müller-Eberhard H. J. Formation of the initial C3 convertase of the alternative complement pathway. Acquisition of C3b-like activities by spontaneous hydrolysis of the putative thioester in native C3. J Exp Med. 1981 Sep 1;154(3):856–867. doi: 10.1084/jem.154.3.856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pinckard R. N., Olson M. S., Giclas P. C., Terry R., Boyer J. T., O'Rourke R. A. Consumption of classical complement components by heart subcellular membranes in vitro and in patients after acute myocardial infarction. J Clin Invest. 1975 Sep;56(3):740–750. doi: 10.1172/JCI108145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Roffe L. M., Maini R. N., Cohen M. L., Meretey K. Measurement of immunoglobulin production by peripheral blood mononuclear cells in vitro using a solid-phase immunoradiometric assay. J Immunol Methods. 1981;43(1):59–66. doi: 10.1016/0022-1759(81)90036-3. [DOI] [PubMed] [Google Scholar]
  11. Strunk R. C., Kunke K. S., Hollister J. R., Musson R. A. Contact with specific surfaces stimulates the production of the second component of complement (C2) in human peripheral blood monocytes via a lymphocyte factor. J Reticuloendothel Soc. 1982 Oct;32(4):297–309. [PubMed] [Google Scholar]
  12. Strunk R. C., Kunke K. S., Musson R. A. Lack of requirement for spreading for macrophages to synthesize complement. J Reticuloendothel Soc. 1980 Nov;28(5):483–493. [PubMed] [Google Scholar]
  13. Sundsmo J. S., Götze O. Human monocyte spreading induced by factor Bb of the alternative pathway of complement activation. A possible role for C5 in monocyte spreading. J Exp Med. 1981 Sep 1;154(3):763–777. doi: 10.1084/jem.154.3.763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tack B. D., Prahl J. W. Third component of human complement: purification from plasma and physicochemical characterization. Biochemistry. 1976 Oct 5;15(20):4513–4521. doi: 10.1021/bi00665a028. [DOI] [PubMed] [Google Scholar]
  15. Whaley K. Biosynthesis of the complement components and the regulatory proteins of the alternative complement pathway by human peripheral blood monocytes. J Exp Med. 1980 Mar 1;151(3):501–516. doi: 10.1084/jem.151.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. von Zabern I., Nolte R., Vogt W. Treatment of human complement components C4 and C3 with amines or chaotropic ions. Evidence of a functional and structural change that provides uncleaved C4 and C3 with properties of their soluble activated froms, C4b and C3b. Scand J Immunol. 1981;13(5):413–431. doi: 10.1111/j.1365-3083.1981.tb00152.x. [DOI] [PubMed] [Google Scholar]

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