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. 1983 May;49(1):95–102.

Lymphocyte chemotaxis in inflammation. VI. Lyt phenotype analysis of effector cells responsible for producing murine lymphocyte chemotactic factor.

Y Shimokawa, K Miura, M Hifumi, H Hayashi
PMCID: PMC1454092  PMID: 6220965

Abstract

Murine lymphocyte chemotactic factor (LCF) was demonstrated in various culture fluids of C3H/HeN lymphoid cells stimulated with specific soluble protein antigen, mitogen or alloantigenic cells. Further experiments, using monoclonal anti-Thy 1.2, anti-Lyt 1.1 and anti-Lyt 2.1 antibodies for negative selection with complement (C), were carried out to characterize the effector-cell populations responsible for producing LCF after these stimuli. Treatment of sensitized lymph node (LN) cells with either anti-Thy 1.2, or anti-Lyt 1.1 and C resulted in an almost complete elimination of the capacity to produce LCF after dinitrophenylated-ovalbumin-stimulation. In addition, spleen cells treated with these antibodies and C before stimulation with either alloantigen (irradiated C57BL/6 spleen cells or concanavalin A [Con A]) yielded almost the same results as those for LN cells. In contrast, depletion of Lyt cells, under conditions which fully abrogated the generation of cytotoxic T cells in primary mixed-lymphocyte culture (MLC) and the cytotoxic activity of the cells generated in MLC, had little or no ability to eliminate LCF production in either system. It was thus suggested that Lyt 1+2- T-cell subpopulations were primarily responsible for LCF production after stimulation with either specific protein antigen, alloantigen, or Con A.

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Selected References

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  1. Adelman N. E., Ksiazek J., Yoshida T., Cohen S. Lymphoid sources of murine migration inhibition factor. J Immunol. 1980 Feb;124(2):825–830. [PubMed] [Google Scholar]
  2. Cantor H., Boyse E. A. Regulation of cellular and humoral immune responses by T-cell subclasses. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):23–32. doi: 10.1101/sqb.1977.041.01.006. [DOI] [PubMed] [Google Scholar]
  3. Castes M., Borderie N., Orbach-Arbouys S. Modification of the migration of mouse lymphoid cells by BCG: a factor stimulating T-cell migration. Cell Immunol. 1978 Sep 15;40(1):37–45. doi: 10.1016/0008-8749(78)90313-1. [DOI] [PubMed] [Google Scholar]
  4. Center D. M., Cruikshank W. Modulation of lymphocyte migration by human lymphokines. I. Identification and characterization of chemoattractant activity for lymphocytes from mitogen-stimulated mononuclear cells. J Immunol. 1982 Jun;128(6):2563–2568. [PubMed] [Google Scholar]
  5. Claudy A. L., Schmitt D., Viac J., Alario A., Staquet M. J., Thivolet J. Morphological, immunological and immunocytochemical identification of lymphocytes extracted from cutaneous infiltrates. Clin Exp Immunol. 1976 Jan;23(1):61–68. [PMC free article] [PubMed] [Google Scholar]
  6. Eardley D. D., Shen F. W., Gershon R. K., Ruddle N. H. Lymphotoxin production by subsets of T cells. J Immunol. 1980 Mar;124(3):1199–1202. [PubMed] [Google Scholar]
  7. El-Naggar A. K., Van Epps D. E., Williams R. C., Jr Human-B and T-lymphocyte locomotion in response to casein, C5a, and f-met-leu-phe. Cell Immunol. 1980 Dec;56(2):365–373. doi: 10.1016/0008-8749(80)90112-4. [DOI] [PubMed] [Google Scholar]
  8. Higuchi Y., Honda M., Hayashi H. Production of chemotactic factor for lymphocytes by neutral SH-dependent protease of rabbit PMN leukocytes from immunoglobulins, especially IgM. Cell Immunol. 1975 Jan;15(1):100–108. doi: 10.1016/0008-8749(75)90168-9. [DOI] [PubMed] [Google Scholar]
  9. Higuchi Y., Ishida M., Hayashi H. A lymphocyte chemotactic peptide released from immunoglobulin G by neutrophil neutral thiol protease. Cell Immunol. 1979 Sep 1;46(2):297–308. doi: 10.1016/0008-8749(79)90418-0. [DOI] [PubMed] [Google Scholar]
  10. Jandinski J., Cantor H., Tadakuma T., Peavy D. L., Pierce C. W. Separation of helper T cells from suppressor T cells expressing different Ly components. I. Polyclonal activation: suppressor and helper activities are inherent properties of distinct T-cell subclasses. J Exp Med. 1976 Jun 1;143(6):1382–1390. doi: 10.1084/jem.143.6.1382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kühner A. L., Cantor H., David J. R. Ly phenotype of lymphocytes producing murine migration inhibitory factor (MIF). J Immunol. 1980 Sep;125(3):1117–1119. [PubMed] [Google Scholar]
  12. Ledbetter J. A., Herzenberg L. A. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63–90. doi: 10.1111/j.1600-065x.1979.tb00289.x. [DOI] [PubMed] [Google Scholar]
  13. Ly I. A., Mishell R. I. Separation of mouse spleen cells by passage through columns of sephadex G-10. J Immunol Methods. 1974 Aug;5(3):239–247. doi: 10.1016/0022-1759(74)90108-2. [DOI] [PubMed] [Google Scholar]
  14. McKenzie I. F., Potter T. Murine lymphocyte surface antigens. Adv Immunol. 1979;27:179–338. doi: 10.1016/s0065-2776(08)60263-1. [DOI] [PubMed] [Google Scholar]
  15. Nakayama E., Shiku H., Stockert E., Oettgen H. F., Old L. J. Cytotoxic T cells: Lyt phenotype and blocking of killing activity by Lyt antisera. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1977–1981. doi: 10.1073/pnas.76.4.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. O'Neill G. J., Parrott D. M. Locomotion of human lymphoid cells. I. Effect of culture and con A on T and non-T lymphocytes. Cell Immunol. 1977 Oct;33(2):257–267. doi: 10.1016/0008-8749(77)90156-3. [DOI] [PubMed] [Google Scholar]
  17. Peck A. B., Bach F. H. Mouse cell-mediated lympholysis assay in serum-free and mouse serum-supplemented media: culture conditions and genetic factors. Scand J Immunol. 1975;4(1):53–62. doi: 10.1111/j.1365-3083.1975.tb02599.x. [DOI] [PubMed] [Google Scholar]
  18. Roehm N. W., Sidky Y. A., Auerbach R. Lyt phenotype analysis of the effector cells responsible for evoking lymphocyte-induced angiogenesis (LIA). Cell Immunol. 1981 Sep 15;63(2):272–278. doi: 10.1016/0008-8749(81)90006-x. [DOI] [PubMed] [Google Scholar]
  19. Roos D., Loos J. A. Changes in the carbohydrate metabolism of mitogenically stimulated human peripheral lymphocytes. I. Stimulation by phytohaemagglutinin. Biochim Biophys Acta. 1970 Dec 29;222(3):565–582. doi: 10.1016/0304-4165(70)90182-0. [DOI] [PubMed] [Google Scholar]
  20. Russell R. J., Wilkinson P. C., Sless F., Parrott D. M. Chemotaxis of lymphoblasts. Nature. 1975 Aug 21;256(5519):646–648. doi: 10.1038/256646a0. [DOI] [PubMed] [Google Scholar]
  21. Shiku H., Kisielow P., Bean M. A., Takahashi T., Boyse E. A., Oettgen H. F., Old L. J. Expression of T-cell differentiation antigens on effector cells in cell-mediated cytotoxicity in vitro. Evidence for functional heterogeneity related to the surface phenotype of T cells. J Exp Med. 1975 Jan 1;141(1):227–241. doi: 10.1084/jem.141.1.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Vadas M. A., Miller J. F., McKenzie I. F., Chism S. E., Shen F. W., Boyse E. A., Gamble J. R., Whitelaw A. M. Ly and Ia antigen phenotypes of T cells involved in delayed-type hypersensitivity and in suppression. J Exp Med. 1976 Jul 1;144(1):10–19. doi: 10.1084/jem.144.1.10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ward P. A., Offen C. D., Montgomery J. R. Chemoattractants of leukocytes, with special reference to lymphocytes. Fed Proc. 1971 Nov-Dec;30(6):1721–1724. [PubMed] [Google Scholar]
  24. Ward P. A., Unanue E. R., Goralnick S. J., Schreiner G. F. Chemotaxis of rat lymphocytes. J Immunol. 1977 Aug;119(2):416–421. [PubMed] [Google Scholar]
  25. Wilkinson P. C., Roberts J. A., Russell R. J., McLoughlin M. Chemotaxis of mitogen-activated human lymphocytes and the effects of membrane-active enzymes. Clin Exp Immunol. 1976 Aug;25(2):280–287. [PMC free article] [PubMed] [Google Scholar]
  26. Zigmond S. H., Hirsch J. G. Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. J Exp Med. 1973 Feb 1;137(2):387–410. doi: 10.1084/jem.137.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]

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