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. 1983 May;49(1):29–35.

Cytolysis of Sendai virus-infected guinea-pig cells by homologous complement.

H Okada, H Tanaka, N Okada
PMCID: PMC1454102  PMID: 6301976

Abstract

Guinea-pig line 10 tumor (GPL10) cells, which were transplantable in strain 2 guinea-pigs, gained the capacity to activate the homologous alternative complement pathway (ACP) following infection with ultraviolet-irradiated Sendai virus (UV-SV). The ACP activation of homologous serum on the UV-SV-infected GPL10 cells (UV-SV-GPL10 cells) resulted in cytolysis of the tumor cells. This indicates that UV-SV infection induced not only the capacity to activate complement on the GPL10 cells, but also made them sensitive to complement attack, because GPL10 cells have been demonstrated to be resistant to the reaction of homologous complement via the classical pathway in the presence of rabbit antibody. Furthermore, UV-SV-GPL10 cells prepared with as little as 0.1 plaque-forming unit equivalent of UV-SV per cell showed suppressed growth in syngeneic strain 2 guinea-pigs. The essential role of complement in the elimination of UV-SV-GPL10 cells in vivo was confirmed by demonstrating that UV-SV-GPL10 could grow in guinea-pigs whose complement had been depleted by administration of cobra venom factor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dukor P., Schumann G., Gisler R. H., Dierich M., König W., Hadding U., Bitter-Suermann D. Complement-dependent B-cell activation by cobra venom factor and other mitogens? J Exp Med. 1974 Feb 1;139(2):337–354. doi: 10.1084/jem.139.2.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fearon D. T. Identification of the membrane glycoprotein that is the C3b receptor of the human erythrocyte, polymorphonuclear leukocyte, B lymphocyte, and monocyte. J Exp Med. 1980 Jul 1;152(1):20–30. doi: 10.1084/jem.152.1.20. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fearon D. T. Regulation of the amplification C3 convertase of human complement by an inhibitory protein isolated from human erythrocyte membrane. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5867–5871. doi: 10.1073/pnas.76.11.5867. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hänsch G. M., Hammer C. H., Vanguri P., Shin M. L. Homologous species restriction in lysis of erythrocytes by terminal complement proteins. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5118–5121. doi: 10.1073/pnas.78.8.5118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lambré C., Thibon M. Ability to activate the alternative complement pathway acquired by human and guinea-pig erythrocytes after contact with influenza virus. Ann Immunol (Paris) 1980 Mar-Apr;131C(2):213–221. [PubMed] [Google Scholar]
  6. Mayer M. M. Mechanism of cytolysis by complement. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2954–2958. doi: 10.1073/pnas.69.10.2954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McConnell I., Klein G., Lint T. F., Lachmann P. J. Activation of the alternative complement pathway by human B cell lymphoma lines is associated with Epstein-Barr virus transformation of the cells. Eur J Immunol. 1978 Jul;8(7):453–458. doi: 10.1002/eji.1830080702. [DOI] [PubMed] [Google Scholar]
  8. Nelson R. A., Jr A new concept of immunosuppression in hypersensitivity reactions and in transplantation immunity. Surv Ophthalmol. 1966 Aug;11(4):498–505. [PubMed] [Google Scholar]
  9. Nicholson-Weller A., Burge J., Austen K. F. Purification from guinea pig erythrocyte stroma of a decay-accelerating factor for the classical c3 convertase, C4b,2a. J Immunol. 1981 Nov;127(5):2035–2039. [PubMed] [Google Scholar]
  10. Ohanian S. H., Borsos T., Rapp H. J. Lysis of tumor cells by antibody and complement. 1. Lack of correlation between antigen content and lytic susceptibility. J Natl Cancer Inst. 1973 May;50(5):1313–1320. doi: 10.1093/jnci/50.5.1313. [DOI] [PubMed] [Google Scholar]
  11. Okada H., Baba T. Rosette formation of human erythrocytes on cultured cells of tumour origin and activation of complement by cell membrane. Nature. 1974 Apr 5;248(448):521–522. doi: 10.1038/248521a0. [DOI] [PubMed] [Google Scholar]
  12. Okada H., Okada N. Sendai virus infected cells are readily cytolysed by guinea-pig complement without antibody. Immunology. 1981 Jun;43(2):337–344. [PMC free article] [PubMed] [Google Scholar]
  13. Okada N., Okada H. Activation of complement by spontaneous leukemic cells of AKR mice. Int J Cancer. 1978 Sep 15;22(3):282–287. doi: 10.1002/ijc.2910220310. [DOI] [PubMed] [Google Scholar]
  14. Okada N., Shibuta H., Okada H. Activation of the alternative pathway of guinea pig complement by Sendai virus-treated cells. Microbiol Immunol. 1979;23(7):689–692. doi: 10.1111/j.1348-0421.1979.tb00511.x. [DOI] [PubMed] [Google Scholar]
  15. Sissons J. G., Oldstone M. B., Schreiber R. D. Antibody-independent activation of the alternative complement pathway by measles virus-infected cells. Proc Natl Acad Sci U S A. 1980 Jan;77(1):559–562. doi: 10.1073/pnas.77.1.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yoshikawa Y., Yamanouchi K., Hishiyama M., Kobune K. Lysis of RSV-transformed Japanese quail cells by a factor from normal quail serum. Int J Cancer. 1978 May 15;21(5):658–666. doi: 10.1002/ijc.2910210518. [DOI] [PubMed] [Google Scholar]
  17. Zbar B., Bernstein I. D., Rapp H. J. Suppression of tumor growth at the site of infection with living Bacillus Calmette-Guérin. J Natl Cancer Inst. 1971 Apr;46(4):831–839. [PubMed] [Google Scholar]

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