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. 1987 Dec;62(4):575–580.

Sheep MHC class II molecules. II. Identification and characterization of four distinct subsets of sheep MHC class II molecules.

N K Puri 1, M R Brandon 1
PMCID: PMC1454155  PMID: 3480873

Abstract

A panel of seven monoclonal antibodies, sequential immunoprecipitation and two-dimensional NEPHGE/SDS-PAGE analyses were used to identify and characterize subsets of sheep MHC class II molecules. Using sequential immunoprecipitation four distinct subsets of class II molecules were identified by the monoclonal antibodies SBU.II 28-1, 37-68, 38-27 and 42-20, while another monoclonal antibody, SBU.II 49-1, recognized all four subsets of class II molecules. These four subpopulations of sheep class II molecules displayed different two-dimensional gel profiles and, using splenocytes from four outbred sheep, the class II molecules recognized by SBU.II 28-1, 37-68 and 42-20 showed structurally detectable allelic polymorphism in their beta polypeptides, but no detectable variation in their alpha polypeptides. In contrast, the class II molecules recognized by SBU.II 38-27 showed allelic variation in both their alpha and beta polypeptides. Two-dimensional (2-D) gel analyses of non-glycosylated class II molecules immunoprecipitated by SBU.II 49-1 suggested that approximately 10-12 different class II molecules were expressed by a single sheep. The results of this study show that sheep express class II molecules that can be divided into four structurally and serologically distinct subsets, and provide additional evidence for the subdivision of the sheep MHC class II genetic region into at least three distinct subregions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell J. I., Denny D. W., Jr, McDevitt H. O. Structure and polymorphism of murine and human class II major histocompatibility antigens. Immunol Rev. 1985 Jul;84:51–71. doi: 10.1111/j.1600-065x.1985.tb01125.x. [DOI] [PubMed] [Google Scholar]
  2. Cullen S. E. Isolation and analysis of the murine Ia molecular complex. Methods Enzymol. 1984;108:523–548. doi: 10.1016/s0076-6879(84)08116-7. [DOI] [PubMed] [Google Scholar]
  3. De Kretser T. A., Crumpton M. J., Bodmer J. G., Bodmer W. F. Two-dimensional gel analysis of the polypeptides precipitated by a polymorphic HLA-DR1,2,w6 monoclonal antibody: evidence for a third locus. Eur J Immunol. 1982 Jul;12(7):600–606. doi: 10.1002/eji.1830120713. [DOI] [PubMed] [Google Scholar]
  4. Giles R. C., Capra J. D. Structure, function, and genetics of human class II molecules. Adv Immunol. 1985;37:1–71. doi: 10.1016/s0065-2776(08)60337-5. [DOI] [PubMed] [Google Scholar]
  5. Jones P. P. Analysis of H-2 and Ia antigens by two-dimensional polyacrylamide gel electrophoresis. Methods Enzymol. 1984;108:452–466. doi: 10.1016/s0076-6879(84)08111-8. [DOI] [PubMed] [Google Scholar]
  6. Puri N. K., Gorrell M. D., Brandon M. R. Sheep MHC class II molecules. I. Immunochemical characterization. Immunology. 1987 Dec;62(4):567–573. [PMC free article] [PubMed] [Google Scholar]
  7. Puri N. K., Mackay C. R., Brandon M. R. Sheep lymphocyte antigens (OLA). II. Major histocompatibility complex class II molecules. Immunology. 1985 Dec;56(4):725–733. [PMC free article] [PubMed] [Google Scholar]
  8. Scott P. C., Choi C. L., Brandon M. R. Genetic organization of the ovine MHC class II region. Immunogenetics. 1987;25(2):116–122. doi: 10.1007/BF00364277. [DOI] [PubMed] [Google Scholar]
  9. Sittisombut N., Knight K. L. Rabbit major histocompatibility complex. I. Isolation and characterization of three subregions of class II genes. J Immunol. 1986 Mar 1;136(5):1871–1875. [PubMed] [Google Scholar]
  10. Sung E., Duncan W. R., Streilein J. W., Jones P. P. Detection of two distinct class II alpha:beta:Ii complexes in the Syrian hamster. Immunogenetics. 1982;16(5):425–433. doi: 10.1007/BF00372101. [DOI] [PubMed] [Google Scholar]
  11. Trowsdale J. Genetics and polymorphism: class II antigens. Br Med Bull. 1987 Jan;43(1):15–36. doi: 10.1093/oxfordjournals.bmb.a072168. [DOI] [PubMed] [Google Scholar]
  12. Watson A. J., DeMars R., Trowbridge I. S., Bach F. H. Detection of a novel human class II HLA antigen. 1983 Jul 28-Aug 3Nature. 304(5924):358–361. doi: 10.1038/304358a0. [DOI] [PubMed] [Google Scholar]
  13. de Kretser T., Crumpton M. J., Bodmer J. G., Bodmer W. F. Biochemical analysis of the polymorphism of three HLA-D region antigens. Mol Biol Med. 1983 Jul;1(1):59–76. [PubMed] [Google Scholar]

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