Abstract
Two different methods, a rosette assay and a direct binding assay, have been employed in an examination of the binding of human IgG1 to mouse macrophages. In both cases, inhibition of IgG binding was demonstrated by Fc (CH2 + CH3 domains) and pFc' (CH3 domains) fragments of human IgG. In a homologous system, the binding of 125I-human IgG to human peripheral-blood monocytes was inhibited by the Fc fragment whereas the pFc' fragment was inactive. Scatchard plot analysis of the binding data from both the heterologous and homologous systems allowed association constants and numbers of receptors per cell to be calculated. A more thorough examination of the possible location of IgG Fc-receptor binding sites was made using less orthodox proteolytic cleavage fragments of IgG. The site on human IgG1 responsible for binding to mouse macrophage Fc receptors was confirmed to be within the CH3 domains. Human IgG1 binding to homologous monocytes was shown, using a dimeric C gamma 2 domain fragment, to be via the CH2 domains, and was dependent on the integrity of the covalent interaction between the C gamma 2 domains at the hinge region.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alexander M. D., Andrews J. A., Leslie R. G., Wood N. J. The binding of human and guinea-pig IgG subclasses to homologous macrophage and monocyte Fc receptors. Immunology. 1978 Jul;35(1):115–123. [PMC free article] [PubMed] [Google Scholar]
- Alexander M. D., Leslie R. G., Cohen S. Cytophilic activity of enzymatically derived fragments of guinea pig IgG2. Eur J Immunol. 1976 Feb;6(2):101–107. doi: 10.1002/eji.1830060206. [DOI] [PubMed] [Google Scholar]
- Barnett Foster D. E., Dorrington K. J., Painter R. H. Structure and function of immunoglobulin domains. VIII. An analysis of the structural requirements in human IgG1 for binding to the Fc receptor of human monocytes. J Immunol. 1980 May;124(5):2186–2190. [PubMed] [Google Scholar]
- Berken A., Benacerraf B. Properties of antibodies cytophilic for macrophages. J Exp Med. 1966 Jan 1;123(1):119–144. doi: 10.1084/jem.123.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ciccimarra F., Rosen F. S., Merler E. Localization of the IgG effector site for monocyte receptors. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2081–2083. doi: 10.1073/pnas.72.6.2081. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dissanayake S., Hay F. C. Investigation of the binding site of mouse IgG subclasses to homologous peritoneal macrophages. Immunology. 1975 Dec;29(6):1111–1118. [PMC free article] [PubMed] [Google Scholar]
- Dorrington K. J. Properties of the Fc receptor on macrophages. Immunol Commun. 1976;5(4):263–280. doi: 10.3109/08820137609044280. [DOI] [PubMed] [Google Scholar]
- Edelman G. M., Cunningham B. A., Gall W. E., Gottlieb P. D., Rutishauser U., Waxdal M. J. The covalent structure of an entire gammaG immunoglobulin molecule. Proc Natl Acad Sci U S A. 1969 May;63(1):78–85. doi: 10.1073/pnas.63.1.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ganczakowski M., Leslie R. G. The binding of rabbit IgG and its enzymatically derived fragments to homologous peritoneal macrophages. Immunology. 1979 Mar;36(3):487–494. [PMC free article] [PubMed] [Google Scholar]
- Haeffner-Cavaillon N., Dorrington K. J., Klein M. Studies on the Fc gamma receptor of the murine macrophage-like cell line P388D1. II. Binding of human IgG subclass proteins and their proteolytic fragments. J Immunol. 1979 Nov;123(5):1914–1919. [PubMed] [Google Scholar]
- Haeffner-Cavaillon N., Klein M., Dorrington K. J. Studies on the Fc gamma receptor of the murine macrophage-like cell line P388D1. I. The binding of homologous and heterologous immunoglobulin G1. J Immunol. 1979 Nov;123(5):1905–1913. [PubMed] [Google Scholar]
- Hunneyball I. M., Stanworth D. R. Subfragmentation of the Fc fragment of human IgG1 myeloma protein by thermolysin. Immunology. 1976 Apr;30(4):579–586. [PMC free article] [PubMed] [Google Scholar]
- Leslie R. G., Cohen S. Cytophilic activity of IgG2 from sera of unimmunized guinea-pigs. Immunology. 1974 Oct;27(4):577–587. [PMC free article] [PubMed] [Google Scholar]
- Leslie R. G., Niemetz A. H. Species specificity in the binding of IgG to macrophages. Immunology. 1979 Aug;37(4):835–840. [PMC free article] [PubMed] [Google Scholar]
- Matthews N., Stewart G., Stanworth D. R. Characterisation of a new tryptic sub-fragment of human and rabbit IgG Fc fragment. Immunochemistry. 1971 Oct;8(10):973–981. doi: 10.1016/0019-2791(71)90435-6. [DOI] [PubMed] [Google Scholar]
- Okafor G. O., Turner M. W., Hay F. C. Localisation of monocyte binding site of human immunoglobulin G. Nature. 1974 Mar 15;248(445):228–230. doi: 10.1038/248228a0. [DOI] [PubMed] [Google Scholar]
- Ovary Z., Saluk P. H., Quijada L., Lamm M. E. Biologic activities of rabbit immunoglobulin G in relation to domains of the Fc region. J Immunol. 1976 May;116(5):1265–1271. [PubMed] [Google Scholar]
- Ratcliffe A., Stanworth D. R. The use of synthetic gamma-chain peptides in the localization of the binding site(s) on human IgG1 for the Fc receptors of homologous monocytes and heterologous mouse macrophages. Immunol Lett. 1982 Apr;4(4):215–221. doi: 10.1016/0165-2478(82)90017-7. [DOI] [PubMed] [Google Scholar]
- Turner M. W., Bennich H. Subfragments from the Fc fragment of human immunoglobulin G. Isolation and physicochemical charaterization. Biochem J. 1968 Mar;107(2):171–178. doi: 10.1042/bj1070171. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yasmeen D., Ellerson J. R., Dorrington K. J., Painter R. H. Evidence for the domain hypothesis: location of the site of cytophilic activity toward guinea pig macrophages in the C H 3 homology region of human immunoglobulin G. J Immunol. 1973 Jun;110(6):1706–1709. [PubMed] [Google Scholar]
- Yasmeen D., Ellerson J. R., Dorrington K. J., Painter R. H. The structure and function of immunoglobulin domains. IV. The distribution of some effector functions among the Cgamma2 and Cgamma3 homology regions of human immunoglobulin G1. J Immunol. 1976 Feb;116(2):518–526. [PubMed] [Google Scholar]