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. 1983 Nov;50(3):327–334.

Antibody-dependent cellular cytotoxicity (K) and natural killing (NK) in B-suppressed germ-free nude rats.

D Chassoux, J P Kolb, H Bazin, I C MacLennan
PMCID: PMC1454252  PMID: 6605300

Abstract

The influence of the thymus and the possible requirement for surface immunoglobulin expression for the development of K and NK activity were assessed in rats. This species was chosen in preference to mice as they show good levels of K-cell activity. Studies were carried out in athymic (rnu/rnu) rats some of which were treated from birth with rabbit anti-rat IgM antibody to suppress B-cell development. The results indicate that normal levels of both K and NK activity develop in the spleens of 6-8-week-old athymic rats, which do not contain cells expressing surface membrane immunoglobulin. While K and NK cells show characteristics of the lymphoid lineage, neither the thymus nor surface membrane immunoglobulin expression appears to be required for development of normal levels of these cytolytic activities.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bazin H., Beckers A., Querinjean P. Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur J Immunol. 1974 Jan;4(1):44–48. doi: 10.1002/eji.1830040112. [DOI] [PubMed] [Google Scholar]
  2. Bazin H., Beckers A., Urbain-Vansanten G., Pauwels R., Bruyns C., Tilkin A. F., Platteau B., Urbain J. Transplantable IgD immunoglobulin-secreting tumors in rat. J Immunol. 1978 Nov;121(5):2077–2082. [PubMed] [Google Scholar]
  3. Bazin H., Platteau B., Beckers A., Pauwels R. Differential effect of neonatal injections of anti-mu or anti-delta antibodies on the synthesis of IgM, IgD, IgE, IgA, IgG1, IgG2a, IgG2b, and IgG2c immunoglobulin classes. J Immunol. 1978 Nov;121(5):2083–2087. [PubMed] [Google Scholar]
  4. Beverley P. C., Callard R. E. Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol. 1981 Apr;11(4):329–334. doi: 10.1002/eji.1830110412. [DOI] [PubMed] [Google Scholar]
  5. Brodt P., Gordon J. Antitumor immunity in B-lymphocyte-deprived mice. II. In vitro studies. Cell Immunol. 1981 Nov 15;65(1):20–32. doi: 10.1016/0008-8749(81)90048-4. [DOI] [PubMed] [Google Scholar]
  6. Chi D. S., Thorbecke G. J. Cytotoxicity to allogeneic cells in the chicken. III. Antibody-dependent cell-mediated cytotoxicity in normal and agammaglobulinemic chickens. Cell Immunol. 1981 Nov 1;64(2):258–266. doi: 10.1016/0008-8749(81)90478-0. [DOI] [PubMed] [Google Scholar]
  7. Eremin O., Kraft D., Coombs R. R., Franks D., Ashby J., Plumb D. Surface characteristics of the human K (killer) lymphocyte. Int Arch Allergy Appl Immunol. 1977;55(1-6):112–125. doi: 10.1159/000231917. [DOI] [PubMed] [Google Scholar]
  8. Fast L. D., Hansen J. A., Newman W. Evidence for T cell nature and heterogeneity within natural killer (NK) and antibody-dependent cellular cytotoxicity (ADCC) effectors: a comparison with cytolytic T lymphocytes (CTL). J Immunol. 1981 Aug;127(2):448–452. [PubMed] [Google Scholar]
  9. Festing M. F., May D., Connors T. A., Lovell D., Sparrow S. An athymic nude mutation in the rat. Nature. 1978 Jul 27;274(5669):365–366. doi: 10.1038/274365a0. [DOI] [PubMed] [Google Scholar]
  10. Gidlund M., Ojo E. A., Orn A., Wigzell H., Murgita R. A. Severe suppression of the B-cell system has no impact on the maturation of natural killer cells in mice. Scand J Immunol. 1979;9(2):167–173. doi: 10.1111/j.1365-3083.1979.tb02719.x. [DOI] [PubMed] [Google Scholar]
  11. Golstein P., Fewtrell C. Functional fractionation of human cytotoxic cells using differences in their cation requirements. Nature. 1975 Jun 5;255(5508):491–493. doi: 10.1038/255491a0. [DOI] [PubMed] [Google Scholar]
  12. Greenberg A. H., Hudson L., Shen L., Roitt I. M. Antibody-dependent cell-mediated cytotoxicity due to a "null" lymphoid cell. Nat New Biol. 1973 Mar 28;242(117):111–113. doi: 10.1038/newbio242111a0. [DOI] [PubMed] [Google Scholar]
  13. Holm G. Lysis of antibody-treated human erythrocytes by human leukocytes and macrophages in tissue culture. Int Arch Allergy Appl Immunol. 1972;43(5):671–682. doi: 10.1159/000230883. [DOI] [PubMed] [Google Scholar]
  14. Hunt S. V., Williams A. F. The origin of cell surface immunoglobulin of marrow-derived and thymus-derived lymphocytes of the rat. J Exp Med. 1974 Mar 1;139(3):479–496. doi: 10.1084/jem.139.3.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kim Y. B., Huh N. D., Koren H. S., Amos D. B. Natural killing (NK) and antibody-dependent cellular cytotoxicity (ADCC) in specific pathogen-free (SPF) miniature swine and germfree piglets. I. Comparison of NK and ADCC. J Immunol. 1980 Aug;125(2):755–762. [PubMed] [Google Scholar]
  16. Koren H. S., Amos D. B., Buckley R. H. Natural killing in immunodeficient patients. J Immunol. 1978 Mar;120(3):796–799. [PubMed] [Google Scholar]
  17. Koren H. S., Williams M. S. Natural killing and antibody-dependent cellular cytotoxicity are mediated by different mechanisms and by different cells. J Immunol. 1978 Nov;121(5):1956–1960. [PubMed] [Google Scholar]
  18. Kumararatne D. S., Bazin H., MacLennan I. C. Marginal zones: the major B cell compartment of rat spleens. Eur J Immunol. 1981 Nov;11(11):858–864. doi: 10.1002/eji.1830111103. [DOI] [PubMed] [Google Scholar]
  19. Lipinski M., Virelizier J. L., Tursz T., Griscelli C. Natural killer and killer cell activities in patients with primary immunodeficiencies or defects in immune interferon production. Eur J Immunol. 1980 Apr;10(4):246–249. doi: 10.1002/eji.1830100405. [DOI] [PubMed] [Google Scholar]
  20. MacDonald H. R., Bonnard G. D., Sordat B., Zawodnik S. A. Antibody-dependent cell-mediated cytotoxicity: heterogeneity of effector cells in human peripheral blood. Scand J Immunol. 1975 Sep;4(5-6):487–497. doi: 10.1111/j.1365-3083.1975.tb02654.x. [DOI] [PubMed] [Google Scholar]
  21. MacDonald H. R., Lees R. K., Glasebrook A. L., Sordat B. Interleukin 2 production by lymphoid cells from congenitally athymic (nu/nu) mice. J Immunol. 1982 Aug;129(2):521–525. [PubMed] [Google Scholar]
  22. MacLennan I. C. Antibody in the induction and inhibition of lymphocyte cytotoxicity. Transplant Rev. 1972;13:67–90. doi: 10.1111/j.1600-065x.1972.tb00060.x. [DOI] [PubMed] [Google Scholar]
  23. MacLennan I. C., Golstein P. Recognition by cytolytic T and K cells: identification in both systems of a divalent cation-independent, cytochalasin A-sensitive step. J Immunol. 1978 Dec;121(6):2542–2546. [PubMed] [Google Scholar]
  24. MacLennan I. C., Harding B. Failure of certain cytotoxic lymphocytes to respond mitotically to phytohaemagglutinin. Nature. 1970 Sep 19;227(5264):1246–1248. doi: 10.1038/2271246a0. [DOI] [PubMed] [Google Scholar]
  25. Perlmann P., Perlmann H., Wigzell H. Lymphocyte mediated cytotoxicity in vitro. Induction and inhibition by humoral antibody and nature of effector cells. Transplant Rev. 1972;13:91–114. doi: 10.1111/j.1600-065x.1972.tb00061.x. [DOI] [PubMed] [Google Scholar]
  26. Ritz J., Nadler L. M., Bhan A. K., Notis-McConarty J., Pesando J. M., Schlossman S. F. Expression of common acute lymphoblastic leukemia antigen (CALLA) by lymphomas of B-cell and T-cell lineage. Blood. 1981 Sep;58(3):648–652. [PubMed] [Google Scholar]
  27. Stein H., Bonk A., Tolksdorf G., Lennert K., Rodt H., Gerdes J. Immunohistologic analysis of the organization of normal lymphoid tissue and non-Hodgkin's lymphomas. J Histochem Cytochem. 1980 Aug;28(8):746–760. doi: 10.1177/28.8.7003001. [DOI] [PubMed] [Google Scholar]
  28. Sulica A., Gherman M., Galatiuc C., Manciulea M., Herberman R. B. Inhibition of human natural killer cell activity by cytophilic immunoglobulin G. J Immunol. 1982 Mar;128(3):1031–1036. [PubMed] [Google Scholar]
  29. Tada M., Hinuma S., Abo T., Kumagai K. Murine antibody-dependent cell-mediated cytotoxicity: failure to detect effector cells equivalent to human K cells. J Immunol. 1980 Apr;124(4):1929–1936. [PubMed] [Google Scholar]
  30. Timonen T., Ortaldo J. R., Herberman R. B. Characteristics of human large granular lymphocytes and relationship to natural killer and K cells. J Exp Med. 1981 Mar 1;153(3):569–582. doi: 10.1084/jem.153.3.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. West W. H., Cannon G. B., Kay H. D., Bonnard G. D., Herberman R. B. Natural cytotoxic reactivity of human lymphocytes against a myeloid cell line: characterization of effector cells. J Immunol. 1977 Jan;118(1):355–361. [PubMed] [Google Scholar]
  32. Widmer M. B., MacDonald H. R., Cerottini J. C. Limiting dilution analysis of alloantigen-reactive T lymphocytes. VI. Ontogeny of cytolytic T lymphocyte precursors in the thymus. Thymus. 1981 Feb;2(4-5):245–255. [PubMed] [Google Scholar]
  33. de Jong W. H., Steerenberg P. A., Ursem P. S., Osterhaus A. D., Vos J. G., Ruitenberg E. J. The athymic nude rat. III. Natural cell-mediated cytotoxicity. Clin Immunol Immunopathol. 1980 Oct;17(2):163–172. doi: 10.1016/0090-1229(80)90084-7. [DOI] [PubMed] [Google Scholar]

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