Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1983 Nov;50(3):369–376.

Immunogenicity of transfer RNA isolated from a two-heptose rough mutant of Salmonella typhimurium LT2 in mouse typhoid infection.

E Kita, S Kashiba
PMCID: PMC1454259  PMID: 6195095

Abstract

Transfer ribonucleic acid (tRNA) was isolated from a two-heptose mutant of Salmonella typhimurium LT2 (strain SL1004) and was found to afford 100% mouse protection against challenge with 1000 LD50 of strain LT2. The intraperitoneal minimum effective dose of tRNA was 5 micrograms RNA per mouse and this dose was significantly lower than that of ribosomal RNA for ddY mouse strain. The protective immunity was independent of the presence of antibodies to cell-surface antigens, and was transferred mainly by T cells. The protective moiety of tRNA was sensitive to ribonuclease digestion which resulted in 85% reduction in the mouse survival rate, but was completely resistant to protease digestion. The present study demonstrates that the immunogenic activity of salmonella RNA is present in both ribosomal RNA and tRNA.

Full text

PDF
369

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonavida B., Fuchs S., Sela M. Antibodies to transfer RNA obtained with covalently linked tRNA conjugates. Biochem Biophys Res Commun. 1970 Dec 9;41(5):1335–1341. doi: 10.1016/0006-291x(70)90235-4. [DOI] [PubMed] [Google Scholar]
  2. Butler R. C., Friedman H., Specter S. C., Eisenstein T. K. Induction of immunoenhancing factors for murine splenocyte cultures by Salmonella typhimurium ribosome and ribonucleic acid extracts. Infect Immun. 1981 Jun;32(3):1123–1127. doi: 10.1128/iai.32.3.1123-1127.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eisenstein T. K., Angerman C. R. Immunity to experimental Salmonella infection: studies on the protective capacity and immunogenicity of lipopolysaccharide, acetone-killed cells, and ribosome-rich extracts of Salmonella typhimurium in C3H/HeJ and CD-1 mice. J Immunol. 1978 Sep;121(3):1010–1014. [PubMed] [Google Scholar]
  4. Eisenstein T. K. Evidence for O antigens as the antigenic determinants in "ribosomal" vaccines prepared from Salmonella. Infect Immun. 1975 Aug;12(2):364–377. doi: 10.1128/iai.12.2.364-377.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Field L. H., Parker C. D., Manclark C. R., Berry L. J. Evaluation of a ribosomal vaccine against pertussis. Infect Immun. 1979 May;24(2):346–351. doi: 10.1128/iai.24.2.346-351.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hoops P., Prather N. E., Berry J., Ravel J. M. Evidence for an extrinsic immunogen in effective ribosomal vaccines from Salmonella typhimurium. Infect Immun. 1976 Apr;13(4):1184–1192. doi: 10.1128/iai.13.4.1184-1192.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  8. Karch H., Nixdorff K. Antibody-producing cell responses to an isolated outer membrane protein and to complexes of this antigen with lipopolysaccharide or with vesicles of phospholipids from Proteus mirabilis. Infect Immun. 1981 Mar;31(3):862–867. doi: 10.1128/iai.31.3.862-867.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kerckhaert J. A., Hofhuis F. M., Willers J. M. Influence of cyclophosphamide on delayed hypersensitivity and acquired cellular resistance to Listeria monocytogenes in the mouse. Immunology. 1977 Jun;32(6):1027–1032. [PMC free article] [PubMed] [Google Scholar]
  10. Lin J. H., Berry L. J. The use of strain LT2-Ml in identifying the protective antigens in a Salmonella typhimurium-derived ribosomal vaccine. J Reticuloendothel Soc. 1978 Feb;23(2):135–143. [PubMed] [Google Scholar]
  11. Misfeldt M. L., Johnson W. Identification of protective cell surface proteins in ribosomal fractions from Salmonella typhimurium. Infect Immun. 1979 Jun;24(3):808–816. doi: 10.1128/iai.24.3.808-816.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nakamura K., Mizushima S. Effects of heating in dodecyl sulfate solution on the conformation and electrophoretic mobility of isolated major outer membrane proteins from Escherichia coli K-12. J Biochem. 1976 Dec;80(6):1411–1422. doi: 10.1093/oxfordjournals.jbchem.a131414. [DOI] [PubMed] [Google Scholar]
  13. Nishimura S., Harada F., Narushima U., Seno T. Purification of methionine-, valine-, phenylalanine- and tyrosine-specific tRNA from Escherichia coli. Biochim Biophys Acta. 1967 Jun 20;142(1):133–148. doi: 10.1016/0005-2787(67)90522-9. [DOI] [PubMed] [Google Scholar]
  14. Ruttkowski E., Nixdorff K. Qualitative and quantitative changes in the antibody producing cell response to lipopolysaccharide induced after incorporation of the antigen into bacterial membrane phospholipid vesicles. J Immunol. 1980 Jun;124(6):2548–2551. [PubMed] [Google Scholar]
  15. Venneman M. R., Bigley N. J., Berry L. J. Immunogenicity of Ribonucleic Acid Preparations Obtained from Salmonella typhimurium. Infect Immun. 1970 Jun;1(6):574–582. doi: 10.1128/iai.1.6.574-582.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WEISSBACH A., HURWITZ J. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. I. Identification. J Biol Chem. 1959 Apr;234(4):705–709. [PubMed] [Google Scholar]
  17. Wright B. G., Rebers P. A. Procedure for determining heptose and hexose in lipopolysaccharides. Modification of the cysteine-sulfuric acid method. Anal Biochem. 1972 Oct;49(2):307–319. doi: 10.1016/0003-2697(72)90433-2. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES