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. 1983 Jul;49(3):555–564.

Human Thy-1 antigen: cell surface expression on early T and B lymphocytes.

M A Ritter, C A Sauvage, D Delia
PMCID: PMC1454303  PMID: 6134665

Abstract

Human Thy-1 is present on the surface of only a small proportion of haemopoietic cells (thymus--0.2-10%; bone marrow--0.1-0.5%). We have analysed these Thy-1+ cells in more detail using immunofluorescence on a wide range of human haemopoietic cell lines and fresh leukaemic blasts; both situations where rare cells can become clonally expanded. The data demonstrate that Thy-1 is confined to the early stages of T- and B-lymphocyte development, and is absent from all myeloid cells. The Thy-1+ B cells represent late pre-B (c mu+)/early sIgM+ B cells. The Thy-1+ T cells are situated in the outer thymic cortex. Dual immunofluorescence analysis of FACS separated Thy-1+ thymocytes shows them to have the antigenic phenotype and morphology of prothymocytes/early thymocytes (some overlap seen with CALLA, TdT, OKT6 and OKT1).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bodger M. P., Francis G. E., Delia D., Granger S. M., Janossy G. A monoclonal antibody specific for immature human hemopoietic cells and T lineage cells. J Immunol. 1981 Dec;127(6):2269–2274. [PubMed] [Google Scholar]
  2. Cotmore S. F., Crowhurst S. A., Waterfield M. D. Purification of Thy-1-related glycoproteins from human brain and fibroblasts: comparisons between these molecules and murine glycoproteins carrying Thy-1.1 and Thy-1.2 antigens. Eur J Immunol. 1981 Aug;11(8):597–603. doi: 10.1002/eji.1830110802. [DOI] [PubMed] [Google Scholar]
  3. Crawford J. M., Goldschneider I. THY1 antigen and B lymphocyte differentiation in the rat. J Immunol. 1980 Feb;124(2):969–976. [PubMed] [Google Scholar]
  4. Dalchau R., Fabre J. W. Identification with a monoclonal antibody of a predominantly B lymphocyte-specific determinant of the human leukocyte common antigen. Evidence for structural and possible functional diversity of the human leukocyte common molecule. J Exp Med. 1981 Apr 1;153(4):753–765. doi: 10.1084/jem.153.4.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldschneider I., Gordon L. K., Morris R. J. Demonstration of Thy-1 antigen on pluripotent hemopoietic stem cells in the rat. J Exp Med. 1978 Nov 1;148(5):1351–1366. doi: 10.1084/jem.148.5.1351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Greaves M. F., Janossy G., Peto J., Kay H. Immunologically defined subclasses of acute lymphoblastic leukaemia in children: their relationship to presentation features and prognosis. Br J Haematol. 1981 Jun;48(2):179–197. [PubMed] [Google Scholar]
  7. Greaves M., Delia D., Janossy G., Rapson N., Chessells J., Woods M., Prentice G. Acute lymphoblastic leukaemia associated antigen. IV. Expression on non-leukaemic 'lymphoid' cells. Leuk Res. 1980;4(1):15–32. doi: 10.1016/0145-2126(80)90044-2. [DOI] [PubMed] [Google Scholar]
  8. Greaves M., Delia D., Sutherland R., Rao J., Verbi W., Kemshead J., Hariri G., Goldstein G., Kung P. Expression of the OKT monoclonal antibody defined antigenic determinants in malignancy. Int J Immunopharmacol. 1981;3(3):283–299. doi: 10.1016/0192-0561(81)90022-9. [DOI] [PubMed] [Google Scholar]
  9. Hunt S. V. The presence of Thy-1 on the surface of rat lymphoid stem cells and colony-forming units. Eur J Immunol. 1979 Nov;9(11):853–859. doi: 10.1002/eji.1830091105. [DOI] [PubMed] [Google Scholar]
  10. Kemshead J. T., Ritter M. A., Cotmore S. F., Greaves M. F. Human Thy-1: expression on the cell surface of neuronal and glial cells. Brain Res. 1982 Mar 25;236(2):451–461. doi: 10.1016/0006-8993(82)90727-2. [DOI] [PubMed] [Google Scholar]
  11. Korsmeyer S. J., Hieter P. A., Ravetch J. V., Poplack D. G., Waldmann T. A., Leder P. Developmental hierarchy of immunoglobulin gene rearrangements in human leukemic pre-B-cells. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7096–7100. doi: 10.1073/pnas.78.11.7096. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McMichael A. J., Pilch J. R., Galfré G., Mason D. Y., Fabre J. W., Milstein C. A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur J Immunol. 1979 Mar;9(3):205–210. doi: 10.1002/eji.1830090307. [DOI] [PubMed] [Google Scholar]
  13. Minowada J., Janossy G., Greaves M. F., Tsubota T., Srivastava B. I., Morikawa S., Tatsumi E. Expression of an antigen associated with acute lymphoblastic leukemia in human leukemia-lymphoma cell lines. J Natl Cancer Inst. 1978 Jun;60(6):1269–1277. doi: 10.1093/jnci/60.6.1269. [DOI] [PubMed] [Google Scholar]
  14. Morris R. J., Mancini P. E., Pfeiffer S. E. Thy-1 cell surface antigen on cloned nerve cell lines of the rat and mouse: amount, location and origin of the antigen on the cells. Brain Res. 1980 Jan 20;182(1):119–135. doi: 10.1016/0006-8993(80)90835-5. [DOI] [PubMed] [Google Scholar]
  15. Raff M. C. Two distinct populations of peripheral lymphocytes in mice distinguishable by immunofluorescence. Immunology. 1970 Oct;19(4):637–650. [PMC free article] [PubMed] [Google Scholar]
  16. Ritter M. A., Gordon L. K., Goldschneider I. Distribution of identity of Thy-1-bearing cells during ontogeny in rat hemopoietic and lymphoid tissues. J Immunol. 1978 Dec;121(6):2463–2471. [PubMed] [Google Scholar]
  17. Ritter M. A., Sauvage C. A., Cotmore S. F. The human thymus microenvironment: in vivo identification of thymic nurse cells and other antigenically-distinct subpopulations of epithelial cells. Immunology. 1981 Nov;44(3):439–446. [PMC free article] [PubMed] [Google Scholar]
  18. Ritz J., Pesando J. M., Notis-McConarty J., Lazarus H., Schlossman S. F. A monoclonal antibody to human acute lymphoblastic leukaemia antigen. Nature. 1980 Feb 7;283(5747):583–585. doi: 10.1038/283583a0. [DOI] [PubMed] [Google Scholar]
  19. Saji F., Tanigaki N. A human thy-1-like antigen expressed on cultured leukemic T-cells. Immunogenetics. 1982;15(6):551–563. doi: 10.1007/BF00347049. [DOI] [PubMed] [Google Scholar]

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