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. 1983 Aug;49(4):641–648.

The in-vivo kinetics of lymphoblast localization in the small intestine.

C A Ottaway, R G Bruce, D M Parrott
PMCID: PMC1454320  PMID: 6874002

Abstract

We have studied the in-vivo kinetics of the accumulation of 125I-UdR labelled mesenteric lymphoblasts in the small intestine of mice. The efficiency with which the labelled cells were extracted from the blood and retained by the intestine was quantified by examination of the accumulations observed over the first 4 hr after cell transfer. The kinetic parameters for the uptake and retention of lymphoblasts determined from these early times were found to provide a good approximation to the entire time course of accumulation observed from 1 hr to 22 hr after cell transfer. For normal mice, approximately 1% of lymphoblasts delivered by the blood stream at any given time gained entry to the small intestine and were retained with an average half-time of 6.5 hr. We also studied the accumulation of lymphoblasts in the small intestine of mice undergoing a self-limited enteric infection with the nematode, Trichinella spiralis. There was a greater accumulation of lymphoblasts in the small intestine of these animals. This was the consequence of a prolongation of the half-time for retention of lymphoblasts within the intestine to 15 hr, rather than increased uptake of lymphoblasts from the blood. During a secondary infection with T. spiralis, the half-time for retention of lymphoblasts in the intestine was decreased to 3 hr. These studies show that viewing the accumulation of lymphoblasts as the result of a series of first order kinetic processes provides a suitable model for the migration of lymphoblasts to the small intestine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown P. J., Bruce R. G., Manson-Smith D. F., Parrott D. M. Intestinal mast cell response in thymectomised and normal mice infected with Trichinella spiralis. Vet Immunol Immunopathol. 1981 Apr;2(2):189–198. doi: 10.1016/0165-2427(81)90049-0. [DOI] [PubMed] [Google Scholar]
  2. GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
  3. Guy-Grand D., Griscelli C., Vassalli P. The gut-associated lymphoid system: nature and properties of the large dividing cells. Eur J Immunol. 1974 Jun;4(6):435–443. doi: 10.1002/eji.1830040610. [DOI] [PubMed] [Google Scholar]
  4. Hall J. G., Hopkins J., Orlans E. Studies on the lymphocytes of sheep. III. Destination of lymph-borne immunoblasts in relation to their tissue of origin. Eur J Immunol. 1977 Jan;7(1):30–37. doi: 10.1002/eji.1830070108. [DOI] [PubMed] [Google Scholar]
  5. Hall J. G., Parry D. M., Smith M. E. The distribution and differentiation of lymph-borne immunoblasts after intravenous injection into syngeneic recipients. Cell Tissue Kinet. 1972 May;5(3):269–281. doi: 10.1111/j.1365-2184.1972.tb00365.x. [DOI] [PubMed] [Google Scholar]
  6. Howard J. C. The life-span and recirculation of marrow-derived small lymphocytes from the rat thoracic duct. J Exp Med. 1972 Feb 1;135(2):185–199. doi: 10.1084/jem.135.2.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Husband A. J., Gowans J. L. The origin and antigen-dependent distribution of IgA-containing cells in the intestine. J Exp Med. 1978 Nov 1;148(5):1146–1160. doi: 10.1084/jem.148.5.1146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Manson-Smith D. F., Bruce R. G., Rose M. I., Parrott D. M. Migration of lymphoblasts to the small intestine. III. Strain differences and relationship to distribution and duration of Trichinella spiralis infection. Clin Exp Immunol. 1979 Dec;38(3):475–482. [PMC free article] [PubMed] [Google Scholar]
  9. McWilliams M., Phillips-Quagliata J. M., Lamm M. E. Characteristics of mesenteric lymph node cells homing to gut-associated lymphoid tissue in syngeneic mice. J Immunol. 1975 Jul;115(1):54–58. [PubMed] [Google Scholar]
  10. Ottaway C. A., Manson-Smith D. F., Bruce R. G., Parrott D. M. Regional blood flow and the localization of lymphoblasts in the small intestine of the mouse. II. The effects of a primary enteric infection with Trichinella spiralis. Immunology. 1980 Dec;41(4):963–971. [PMC free article] [PubMed] [Google Scholar]
  11. Ottaway C. A., Parrott D. M. Regional blood flow and its relationship to lymphocyte and lymphoblast traffic during a primary immune reaction. J Exp Med. 1979 Aug 1;150(2):218–230. doi: 10.1084/jem.150.2.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ottaway C. A., Parrott D. M. Regional blood flow and the localisation of lymphoblasts in the small intestine of the mouse: effect of an elemental diet. Gut. 1981 May;22(5):376–382. doi: 10.1136/gut.22.5.376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ottaway C. A., Parrott D. M. Regional blood flow and the localization of lymphoblasts in the small intestine of the mouse. I. Examination of normal small intestine. Immunology. 1980 Dec;41(4):955–961. [PMC free article] [PubMed] [Google Scholar]
  14. Owen R. L., Nemanic P. C., Stevens D. P. Ultrastructural observations on giardiasis in a murine model. I. Intestinal distribution, attachment, and relationship to the immune system of Giardia muris. Gastroenterology. 1979 Apr;76(4):757–769. [PubMed] [Google Scholar]
  15. Parrott D. M., Ferguson A. Selective migration of lymphocytes within the mouse small intestine. Immunology. 1974 Mar;26(3):571–588. [PMC free article] [PubMed] [Google Scholar]
  16. Parrott D. M., Wilkinson P. C. Lymphocyte locomotion and migration. Prog Allergy. 1981;28:193–284. [PubMed] [Google Scholar]
  17. Rose M. L., Parrott D. M., Bruce R. G. I. Effect of Trichinella spiralis infection on the migration of mesenteric lymphoblasts and mesenteric T lymphoblasts in syngeneic mice. Immunology. 1976 Nov;31(5):723–730. [PMC free article] [PubMed] [Google Scholar]
  18. Rose M. L., Parrott D. M., Bruce R. G. Migration of lymphoblasts to the small intestine. II. Divergent migration of mesenteric and peripheral immunoblasts to sites of inflammation in the mouse. Cell Immunol. 1976 Nov;27(1):36–46. doi: 10.1016/0008-8749(76)90151-9. [DOI] [PubMed] [Google Scholar]
  19. Smith M. E., Martin A. F., Ford W. L. Migration of lymphoblasts in the rat. Preferential localization of DNA-synthesizing lymphocytes in particular lymph nodes and other sties. Monogr Allergy. 1980;16:203–232. [PubMed] [Google Scholar]

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