Skip to main content
Immunology logoLink to Immunology
. 1984 Feb;51(2):333–342.

Pre-B cells in bone marrow: size distribution profile, proliferative capacity and peanut agglutinin binding of cytoplasmic mu chain-bearing cell populations in normal and regenerating bone marrow.

D G Osmond, J J Owen
PMCID: PMC1454424  PMID: 6420335

Abstract

Pre-B cell populations in mouse bone marrow, identified by double immunofluorescence labelling of cytoplasmic and surface mu chains (c mu, s mu), have been characterized by cell size, proliferative capacity and the binding of peanut agglutinin (PNA). In the normal steady state of lymphocyte production the size distribution profile of cytocentrifuged c mu + s mu- cells was bimodal. A population of large cells in rapid cell cycle was revealed by arresting cells in mitosis with vincristine. Many c mu + s mu- cells, however, formed a nondividing population of small lymphocytes, resembling s mu + cells in size distribution. During regeneration from sublethal whole body X-irradiation (150 rads) a marked enrichment of large c mu + s mu- cells preceded small c mu + s mu- and s mu + cells; progressive changes in cell size distribution reflected a wave of B lymphocyte genesis. The c mu + s mu- cells in foetal liver resembled those in regenerating marrow. Surface binding of PNA characterised all c mu + s mu- cell populations in normal and regenerating bone marrow and in foetal liver, whereas only a minority of s mu + cells and mu-negative marrow cells bound PNA strongly. The present size distribution analyses allow a correlation with other cytological and functional studies of marrow lymphocyte precursors in defining the place of pre-B cells in B lymphocyte genesis.

Full text

PDF
333

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brahim F., Osmond D. G. Migration of bone marrow lymphocytes demonstrated by selective bone marrow labeling with thymidine-H3. Anat Rec. 1970 Oct;168(2):139–159. doi: 10.1002/ar.1091680202. [DOI] [PubMed] [Google Scholar]
  2. Burrows P. D., Kearney J. F., Lawton A. R., Cooper M. D. Pre-B cells: bone marrow persistence in anti-mu-suppressed mice, conversion to B lymphocytes, and recovery after destruction by cyclophosphamide. J Immunol. 1978 May;120(5):1526–1531. [PubMed] [Google Scholar]
  3. Freitas A. A., Rocha B., Forni L., Coutinho A. Population dynamics of B lymphocytes and their precursors: demonstration of high turnover in the central and peripheral lymphoid organs. J Immunol. 1982 Jan;128(1):54–60. [PubMed] [Google Scholar]
  4. Lafleur L., Miller R. G., Phillips R. A. A quantitative assay for the progenitors of bone marrow-associated lymphocytes. J Exp Med. 1972 Jun 1;135(6):1363–1374. doi: 10.1084/jem.135.6.1363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Landreth K. S., Rosse C., Clagett J. Myelogenous production and maturation of B lymphocytes in the mouse. J Immunol. 1981 Nov;127(5):2027–2034. [PubMed] [Google Scholar]
  6. Lau C. Y., Melchers F., Miller R. G., Phillips R. A. In vitro differentiation of B lymphocytes from pre-B cells. J Immunol. 1979 Apr;122(4):1273–1277. [PubMed] [Google Scholar]
  7. Melchers F. B lymphocyte development in fetal liver. II. Frequencies of precursor B cells during gestation. Eur J Immunol. 1977 Jul;7(7):482–486. doi: 10.1002/eji.1830070715. [DOI] [PubMed] [Google Scholar]
  8. Miller S. C., Osmond D. G. The proliferation of lymphoid cells in guinea-pig bone marrow. Cell Tissue Kinet. 1973 May;6(3):259–269. doi: 10.1111/j.1365-2184.1973.tb01615.x. [DOI] [PubMed] [Google Scholar]
  9. Newman R. A., Boss M. A. Expression of binding sites for peanut agglutinin during murine B lymphocyte differentiation. Immunology. 1980 Jun;40(2):193–200. [PMC free article] [PubMed] [Google Scholar]
  10. Nicola N. A., Burgess A. W., Staber F. G., Johnson G. R., Metcalf D., Battye F. L. Differential expression of lectin receptors during hemopoietic differentiation: enrichment for granulocyte-macrophage progenitor cells. J Cell Physiol. 1980 May;103(2):217–237. doi: 10.1002/jcp.1041030207. [DOI] [PubMed] [Google Scholar]
  11. Osmond D. G., Nossal G. J. Differentiation of lymphocytes in mouse bone marrow. II. Kinetics of maturation and renewal of antiglobulin-binding cells studied by double labeling. Cell Immunol. 1974 Jul;13(1):132–145. doi: 10.1016/0008-8749(74)90233-0. [DOI] [PubMed] [Google Scholar]
  12. Osmond D. G., Roylance P. J., Lee W. R., Ramsell T. G., Yoffey J. M. The effect of unilateral limb shielding on the haemopoietic response of the guinea-pig to gamma irradiation (150 r.). Br J Haematol. 1966 Jul;12(4):365–375. doi: 10.1111/j.1365-2141.1966.tb05646.x. [DOI] [PubMed] [Google Scholar]
  13. Owen J. J., Wright D. E., Habu S., Raff M. C., Cooper M. D. Studies on the generation of B lymphocytes in fetal liver and bone marrow. J Immunol. 1977 Jun;118(6):2067–2072. [PubMed] [Google Scholar]
  14. Paige C. J., Kincade P. W., Shinefeld L. A., Sato V. L. Precursors of murine B lymphocytes. Physical and functional characterization, and distinctions from myeloid stem cells. J Exp Med. 1981 Jan 1;153(1):154–165. doi: 10.1084/jem.153.1.154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pearl E. R., Vogler L. B., Okos A. J., Crist W. M., Lawton A. R., 3rd, Cooper M. D. B lymphocyte precursors in human bone marrow: an analysis of normal individuals and patients with antibody-deficiency states. J Immunol. 1978 Apr;120(4):1169–1175. [PubMed] [Google Scholar]
  16. Raff M. C., Megson M., Owen J. J., Cooper M. D. Early production of intracellular IgM by B-lymphocyte precursors in mouse. Nature. 1976 Jan 22;259(5540):224–226. doi: 10.1038/259224a0. [DOI] [PubMed] [Google Scholar]
  17. Reisner Y., Linker-Israeli M., Sharon N. Separation of mouse thymocytes into two subpopulations by the use of peanut agglutinin. Cell Immunol. 1976 Jul;25(1):129–134. doi: 10.1016/0008-8749(76)90103-9. [DOI] [PubMed] [Google Scholar]
  18. Rose M. L., Birbeck M. S., Wallis V. J., Forrester J. A., Davies A. J. Peanut lectin binding properties of germinal centres of mouse lymphoid tissue. Nature. 1980 Mar 27;284(5754):364–366. doi: 10.1038/284364a0. [DOI] [PubMed] [Google Scholar]
  19. Rosse C. Small lymphocyte and transitional cell populations of the bone marrow; their role in the mediation of immune and hemopoietic progenitor cell functions. Int Rev Cytol. 1976;45:155–290. doi: 10.1016/s0074-7696(08)60080-7. [DOI] [PubMed] [Google Scholar]
  20. Rusthoven J. J., Phillips R. A. Hydroxyurea kills B cell precursors and markedly reduces functional B cell activity in mouse bone marrow. J Immunol. 1980 Feb;124(2):781–786. [PubMed] [Google Scholar]
  21. Wright N. A., Appleton D. R. The metaphase arrest technique. A critical review. Cell Tissue Kinet. 1980 Nov;13(6):643–663. doi: 10.1111/j.1365-2184.1980.tb00503.x. [DOI] [PubMed] [Google Scholar]
  22. Yang W. C., Miller S. C., Osmond D. G. Maturation of bone marrow lymphocytes. II. Development of Fc and complement receptors and surface immunoglobulin studied by rosetting and radioautography. J Exp Med. 1978 Nov 1;148(5):1251–1270. doi: 10.1084/jem.148.5.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES