Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1984 Feb;51(2):295–303.

Lymphocyte chemotaxis in inflammation. IX. Further characterization of lymphocyte chemotactic lymphokines produced by purified protein derivative-stimulation in vitro and in vivo.

S Harita, Y Shimokawa, Y Mibu, M Ishida, H Hayashi
PMCID: PMC1454442  PMID: 6420334

Abstract

As recently reported, one lymphocyte chemotactic factor (beta-LCF, mol. wt. about 27,000) released from activated guinea-pig lymphocytes appeared to be identical to one of the LCFs (LCF-d) isolated from extract of purified protein derivative (PPD)-induced delayed-type hypersensitivity skin reaction sites in guinea-pigs with respect to antigenicity and chemotactic effect for T cells. However, the mol. wt. of LCF-d (about 300,000) was clearly distinct from beta-LCF. The experiments were undertaken to clarify the problem. beta-LCF appeared to be bound to some protein of normal guinea-pig serum (GPS) because the chemotactic activity was revealed in the fraction corresponding to that of LCF-d when the mixtures of beta-LCF with GPS were applied to a Sephadex G-200 column. Additionally, binding experiments using fluorescein isothiocyanate (FITC)-labelled beta-LCF were performed; fluorescence was only detected in the chemotactic fraction. It was thus assumed that the lymphokine (beta-LCF) would be released from activated lymphocytes around the inflammatory tissue, then bound with serum protein exuded in the site and function as LCF-d. The possibility was supported by the evidence that beta-LCF like-chemotactic substance (mol. wt. about 27,000) was dissociated from LCF-d under acid conditions. The factor dissociated from LCF-d was also bound with GPS protein under neutral conditions and converted to high molecular substance resembling LCF-d physiochemically. Furthermore, the chemotactic activity of LCF-d was almost completely absorbed by antibody against GPS. It is thus considered that the chemotactic activity of LCF-d may be attributed to beta-LCF released from activated lymphocytes and that some serum protein which binds beta-LCF may function as a carrier protein in the DTH sites.

Full text

PDF
295

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dardenne M., Pléau J. M., Bach J. F. Evidence of the presence in normal serum of a carrier of the serum thymic factor (FTS). Eur J Immunol. 1980 Feb;10(2):83–86. doi: 10.1002/eji.1830100203. [DOI] [PubMed] [Google Scholar]
  2. Harita S., Shimokawa Y., Hayashi H. Production of two lymphocyte chemotactic factors by antigen-stimulated guinea pig lymphocytes. Int Arch Allergy Appl Immunol. 1983;70(2):118–123. doi: 10.1159/000233308. [DOI] [PubMed] [Google Scholar]
  3. Hayashi H. The intracellular neutral SH-dependent protease associated with inflammatory reactions. Int Rev Cytol. 1975;40:101–151. [PubMed] [Google Scholar]
  4. Higuchi Y., Honda M., Hayashi H. Production of chemotactic factor for lymphocytes by neutral SH-dependent protease of rabbit PMN leukocytes from immunoglobulins, especially IgM. Cell Immunol. 1975 Jan;15(1):100–108. doi: 10.1016/0008-8749(75)90168-9. [DOI] [PubMed] [Google Scholar]
  5. Honda M., Hayashi H. Characterization of three macrophage chemotactic factors from PPD-induced delayed hypersensitivity reaction sites in guinea pigs, with special reference to a chemotactic lymphokine. Am J Pathol. 1982 Aug;108(2):171–183. [PMC free article] [PubMed] [Google Scholar]
  6. Honda M., Miura K., Kuratsu J., Hayashi H. Characterization of a macrophage chemotactic lymphokine produced by purified protein derivative stimulation in vitro and in vivo. Cell Immunol. 1982 Mar 1;67(2):213–228. doi: 10.1016/0008-8749(82)90215-5. [DOI] [PubMed] [Google Scholar]
  7. Hutchins G. M., Sheldon W. H. The pH of inflammatory exudates in acidotic diabetic rabbits. Proc Soc Exp Biol Med. 1972 Jun;140(2):623–627. doi: 10.3181/00379727-140-36517. [DOI] [PubMed] [Google Scholar]
  8. Maeda H. Assay of proteolytic enzymes by the fluorescence polarization technique. Anal Biochem. 1979 Jan 1;92(1):222–227. doi: 10.1016/0003-2697(79)90649-3. [DOI] [PubMed] [Google Scholar]
  9. Morley J., Williams T. J., Slater A. J., Cubitt D., Dumonde D. C. Simultaneous measurements of the accumulation of isotope-labelled protein and erythrocytes in skin reactions of allergic inflammation in the guinea-pig. Immunology. 1972 Aug;23(2):113–135. [PMC free article] [PubMed] [Google Scholar]
  10. POSTLETHWAITE A. E., Snyderman R. Characterization of chemotactic activity produced in vivo by a cell-mediated immune reaction in the guinea pig. J Immunol. 1975 Jan;114(1 Pt 2):274–278. [PubMed] [Google Scholar]
  11. Porath J., Axen R., Ernback S. Chemical coupling of proteins to agarose. Nature. 1967 Sep 30;215(5109):1491–1492. doi: 10.1038/2151491a0. [DOI] [PubMed] [Google Scholar]
  12. Postlethywaite A. E., Townes A. S., Kang A. H. Characterization of macrophage migration inhibitory factor activity produced in vivo by a cell-mediated immune reaction in the guinea pig. J Immunol. 1976 Nov;117(5 Pt 1):1716–1720. [PubMed] [Google Scholar]
  13. Shimokawa Y., Harita S., Hayashi H. Lymphocyte chemotaxis in inflammation. VII. Isolation and purification of chemotactic factors for T lymphocytes from PPD-induced delayed hypersensitivity skin reaction site in the guinea-pig. Immunology. 1984 Feb;51(2):275–285. [PMC free article] [PubMed] [Google Scholar]
  14. Shimokawa Y., Harita S., Higuchi Y., Hayashi H. Lymphocyte chemotaxis in inflammation. IV. Isolation of lymphocyte chemotactic factors from PPD-induced delayed hypersensitivity skin reaction site in the guinea-pig, with special reference to a factor chemotactic for B cells. Br J Exp Pathol. 1982 Aug;63(4):362–368. [PMC free article] [PubMed] [Google Scholar]
  15. Shimokawa Y., Harita S., Mibu Y., Hayashi H. Lymphocyte chemotaxis in inflammation. VIII. Demonstration of lymphocyte chemotactic lymphokines in PPD-induced delayed hypersensitivity skin reaction site in the guinea-pig. Immunology. 1984 Feb;51(2):287–294. [PMC free article] [PubMed] [Google Scholar]
  16. Yamamoto S., Dunn C. J., Willoughby D. A. Studies on delayed hypersensitivity pleural exudates in guinea pigs. I. Demonstration of substances in the cell-free exudate which cause inhibition of mononuclear cell migration in vitro. Immunology. 1976 Apr;30(4):505–511. [PMC free article] [PubMed] [Google Scholar]
  17. Yamamoto S., Ishikura A. Delayed hypersensitivity reactions in guinea-pigs using cotton pellets; demonstration of macrophage migration inhibition activity in the cell free exudate. J Pathol. 1979 Jan;127(1):1–10. doi: 10.1002/path.1711270102. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES