Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1984 Mar;51(3):595–600.

IgG subclass distribution of autoantibodies to DNA and to nuclear ribonucleoproteins in autoimmune diseases.

M Zouali, R Jefferis, A Eyquem
PMCID: PMC1454452  PMID: 6230310

Abstract

Fifty-seven serum samples positive for antibodies to double-stranded DNA (dsDNA), single-stranded DNA (ssDNA) or small nuclear ribonucleoproteins (snRNP) selected from patients with systemic lupus erythematosus and mixed connective tissue disease, were examined for the IgG subclass distribution of these autoantibodies. It was shown that antibodies to dsDNA were relatively restricted to IgG1 and IgG3 subclasses whilst antibodies to ssDNA were equally distributed throughout the four subclasses. Antibodies to snRNP were essentially restricted to the IgG2 isotype. These isotype distribution patterns contrasted with that observed for total serum IgG.

Full text

PDF
595

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alarcon-Segovia D., Ruiz-Arguelles A., Llorente L. Antibody penetration into living cells. II. Anti-ribonucleoprotein IgG penetrates into Tgamma lymphocytes causing their deletion and the abrogation of suppressor function. J Immunol. 1979 May;122(5):1855–1862. [PubMed] [Google Scholar]
  2. Beck O. E. Distribution of virus antibody activity among human IgG subclasses. Clin Exp Immunol. 1981 Mar;43(3):626–632. [PMC free article] [PubMed] [Google Scholar]
  3. Billings P. B., Allen R. W., Jensen F. C., Hoch S. O. Anti-RNP monoclonal antibodies derived from a mouse strain with lupus-like autoimmunity. J Immunol. 1982 Mar;128(3):1176–1180. [PubMed] [Google Scholar]
  4. Dean B. M., Bottazzo G. F., Cudworth A. G. IgG subclass distribution in organ specific autoantibodies. The relationship to complement fixing ability. Clin Exp Immunol. 1983 Apr;52(1):61–66. [PMC free article] [PubMed] [Google Scholar]
  5. Eilat D., Laskov R. Production of anti-RNA antibody by hybridoma cells: purification from mixed immunoglobulin products. Mol Immunol. 1981 Jul;18(7):589–595. doi: 10.1016/0161-5890(81)90029-8. [DOI] [PubMed] [Google Scholar]
  6. Eilat D. Monoclonal autoantibodies: an approach to studying autoimmune disease. Mol Immunol. 1982 Jul;19(7):943–955. doi: 10.1016/0161-5890(82)90360-1. [DOI] [PubMed] [Google Scholar]
  7. Eisenberg R. A., Winfield J. B., Cohen P. L. Subclass restriction of anti-Sm antibodies in MRL mice. J Immunol. 1982 Nov;129(5):2146–2149. [PubMed] [Google Scholar]
  8. Flanagan J. G., Rabbitts T. H. Arrangement of human immunoglobulin heavy chain constant region genes implies evolutionary duplication of a segment containing gamma, epsilon and alpha genes. Nature. 1982 Dec 23;300(5894):709–713. doi: 10.1038/300709a0. [DOI] [PubMed] [Google Scholar]
  9. Froland S. S., Natvig J. B. Surface-bound immunoglobulin on lymphocytes from normal and immunodeficient humans. Scand J Immunol. 1972;1(1):1–12. doi: 10.1111/j.1365-3083.1972.tb03730.x. [DOI] [PubMed] [Google Scholar]
  10. Fulpius B. W., Miskin R., Reich E. Antibodies from myasthenic patients that compete with cholinergic agents for binding to nicotinic receptors. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4326–4330. doi: 10.1073/pnas.77.7.4326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lefvert A. K., Bergström K. Acetylcholine receptor antibody in myasthenia gravis: purification and characterization. Scand J Immunol. 1978;8(6):525–533. doi: 10.1111/j.1365-3083.1978.tb00552.x. [DOI] [PubMed] [Google Scholar]
  12. Lowe J., Bird P., Hardie D., Jefferis R., Ling N. R. Monoclonal antibodies (McAbs) to determinants on human gamma chains: properties of antibodies showing subclass restriction or subclass specificity. Immunology. 1982 Oct;47(2):329–336. [PMC free article] [PubMed] [Google Scholar]
  13. Martinez-Alonso C., Coutinho A., Augustin A. A. Immunoglobulin C-gene expression. I. The commitment to IgG subclass of secretory cells is determined by the quality of the nonspecific stimuli. Eur J Immunol. 1980 Sep;10(9):698–702. doi: 10.1002/eji.1830100908. [DOI] [PubMed] [Google Scholar]
  14. Mattioli M., Reichlin M. Physical association of two nuclear antigens and mutual occurrence of their antibodies: the relationship of the SM and RNAprotein (MO) systems in SLE sera. J Immunol. 1973 May;110(5):1318–1324. [PubMed] [Google Scholar]
  15. Mayumi M., Kuritani T., Kubagawa H., Cooper M. D. IgG subclass expression by human B lymphocytes and plasma cells: B lymphocytes precommitted to IgG subclass can be preferentially induced by polyclonal mitogens with T cell help. J Immunol. 1983 Feb;130(2):671–677. [PubMed] [Google Scholar]
  16. Puritz E. M., Yount W. J., Newell M., Utsinger P. D. Immunoglobulin classes and IgG subclasses of human antinuclear antibodies. A correlation of complement fixation and the nephritis of systemic lupus erythematosus. Clin Immunol Immunopathol. 1973 Nov;2(1):98–113. doi: 10.1016/0090-1229(73)90040-8. [DOI] [PubMed] [Google Scholar]
  17. Reichlin M. Current perspectives on serological reactions in SLE patients. Clin Exp Immunol. 1981 Apr;44(1):1–10. [PMC free article] [PubMed] [Google Scholar]
  18. Sharp G. C., Irvin W. S., May C. M., Holman H. R., McDuffie F. C., Hess E. V., Schmid F. R. Association of antibodies to ribonucleoprotein and Sm antigens with mixed connective-tissue disease, systematic lupus erythematosus and other rheumatic diseases. N Engl J Med. 1976 Nov 18;295(21):1149–1154. doi: 10.1056/NEJM197611182952101. [DOI] [PubMed] [Google Scholar]
  19. Sontheimer R. D., Gilliam J. N. DNA antibody class, subclass, and complement fixation in systemic lupus erythematosus with and without nephritis. Clin Immunol Immunopathol. 1978 Aug;10(4):459–467. doi: 10.1016/0090-1229(78)90158-7. [DOI] [PubMed] [Google Scholar]
  20. Stevens R., Dichek D., Keld B., Heiner D. IgG1 is the predominant subclass of in vivo- and in vitro- produced anti-tetanus toxoid antibodies and also serves as the membrane IgG molecule for delivering inhibitory signals to anti-tetanus toxoid antibody-producing B cells. J Clin Immunol. 1983 Jan;3(1):65–69. doi: 10.1007/BF00919140. [DOI] [PubMed] [Google Scholar]
  21. Teale J. M. Abnormalities in isotype expression in CBA/N mice due to stimulatory environment rather than a B cell defect. J Immunol. 1983 Jan;130(1):72–77. [PubMed] [Google Scholar]
  22. Tojo T., Friou G. J. Lupus nephritis: varying complement-fixing properties of immunoglobulin G antibodies to antigens of cell nuclei. Science. 1968 Aug 30;161(3844):904–906. doi: 10.1126/science.161.3844.904. [DOI] [PubMed] [Google Scholar]
  23. Wiegand R. C., Godson G. N., Radding C. M. Specificity of the S1 nuclease from Aspergillus oryzae. J Biol Chem. 1975 Nov 25;250(22):8848–8855. [PubMed] [Google Scholar]
  24. Yount W. J., Dorner M. M., Kunkel H. G., Kabat E. A. Studies on human antibodies. VI. Selective variations in subgroup composition and genetic markers. J Exp Med. 1968 Mar 1;127(3):633–646. doi: 10.1084/jem.127.3.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yount W. J., Fuller C. R., Simmons J. G. Distribution of IgG subclasses in human B lymphocytes: evidence for dual expression of subclasses in surface and cytoplasmic IgG in minor B lymphocyte subpopulations. J Immunol. 1980 Jan;124(1):431–436. [PubMed] [Google Scholar]
  26. Zouali M., Eyquem A. Expression of anti-idiotypic clones against auto-anti-DNA antibodies in normal individuals. Cell Immunol. 1983 Feb 15;76(1):137–147. doi: 10.1016/0008-8749(83)90356-8. [DOI] [PubMed] [Google Scholar]
  27. van Loghem E., Litwin S. D. Antigenic determinants on immunoglobulins of nonhuman primates. Transplant Proc. 1972 Mar;4(1):129–135. [PubMed] [Google Scholar]
  28. van der Giessen M., Homan W. L., van Kernbeek G., Aalberse R. C., Dieges P. H. Subclass typing of IgG antibodies formed by grass pollen-allergic patients during immunotherapy. Int Arch Allergy Appl Immunol. 1976;50(5):625–640. doi: 10.1159/000231566. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES