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. 1984 Apr;51(4):755–763.

Studies in the enhancement of tumour immunity by coupling strong antigens to tumour cells ('heterogenization of tumours'). Helper T cell clones against PPD help other T cells mount anti-tumour responses to PPD-coupled tumour cells.

D Y Sia, P J Lachmann, K N Leung
PMCID: PMC1454546  PMID: 6200428

Abstract

PPD-reactive T cell clones have been used to analyse the nature of T lymphocytes that are involved in the 'heterogenization' of tumour cells. This is a phenomenon where coupling tumour cells to a strong antigen (in this case PPD) causes an enhanced immune response to tumour-specific antigens to be elicited providing that the host shows T cell immunity to the strong antigen (in this case is BCG positive). Clones of T cells with the Lyt1+2- phenotype which were unable to mediate delayed-type hypersensitivity but which provided efficient help to hapten-primed B cells were found to potentiate anti-tumour immunity in BCG-negative syngeneic mice when immunized with Con-A-PPD coupled, X-irradiated MC6A tumour cells. There therefore appears to be a mechanism whereby a helper T cell response to one antigen can provide help for the generation of a T cell response to a linked antigen which is analogous to the well-known phenomenon of help to hapten primed B cells. Furthermore the clones of T cells that help B cells the best are those that give maximal augmentation of T cell immunity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altman A., Katz D. H. Stimulation of tumor-specific cytotoxic T-lymphocyte responses in vitro by hapten-reactive helper T lymphocytes and the allogeneic effect. Clin Immunol Immunopathol. 1980 Mar;15(3):552–564. doi: 10.1016/0090-1229(80)90066-5. [DOI] [PubMed] [Google Scholar]
  2. Basombrío M. A. Search for common antigenicities among twenty-five sarcomas induced by methylcholanthrene. Cancer Res. 1970 Oct;30(10):2458–2462. [PubMed] [Google Scholar]
  3. Brownstone A., Mitchison N. A., Pitt-Rivers R. Chemical and serological studies with an iodine-containing synthetic immunological determinant 4-hydroxy-3-iodo-5-nitrophenylacetic acid (NIP) and related compounds. Immunology. 1966 May;10(5):465–479. [PMC free article] [PubMed] [Google Scholar]
  4. Gillis S., Ferm M. M., Ou W., Smith K. A. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed] [Google Scholar]
  5. Hamaoka T., Fujiwara H., Teshima K., Aoki H., Yamamoto H., Kitagawa M. Regulatory functions of hapten-reactive helper and suppressor T lymphocytes. III. Amplification of a generation of tumor-specific killer T-lymphocyte activities by suppressor T-cell-depleted hapten-reactive T lymphocytes. J Exp Med. 1979 Jan 1;149(1):185–199. doi: 10.1084/jem.149.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kobayashi H., Sendo F., Kaji H., Shirai T., Saito H., Takeichi N., Hosokawa M., Kodama T. Inhibition of transplanted rat tumors by immunization with identical tumor cells infected with Friend virus. J Natl Cancer Inst. 1970 Jan;44(1):11–19. [PubMed] [Google Scholar]
  7. Lachmann P. J., Sikora K. Coupling PPD to tumour cells enhances their antigenicity in BCG-primed mice. Nature. 1978 Feb 2;271(5644):463–464. doi: 10.1038/271463a0. [DOI] [PubMed] [Google Scholar]
  8. Lachmann P. J., Vyakarnam A., Sikora K. The heterogenization of tumour cells with tuberculin. I. The coupling of tuberculin to cell surfaces using con-A as ligand. Immunology. 1981 Feb;42(2):329–336. [PMC free article] [PubMed] [Google Scholar]
  9. Leung K. N., Ada G. L., McKenzie I. F. Specificity, Ly phenotype, and H-2 compatibility requirements of effector cells in delayed-type hypersensitivity responses to murine influenza virus infection. J Exp Med. 1980 Apr 1;151(4):815–826. doi: 10.1084/jem.151.4.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lindenmann J., Klein P. A. Viral oncolysis: increased immunogenicity of host cell antigen associated with influenza virus. J Exp Med. 1967 Jul 1;126(1):93–108. doi: 10.1084/jem.126.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Martin W. J., Wunderlich J. R., Fletcher F., Inman J. K. Enhanced immunogenicity of chemically-coated syngeneic tumor cells. Proc Natl Acad Sci U S A. 1971 Feb;68(2):469–472. doi: 10.1073/pnas.68.2.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mitchison N. A. The carrier effect in the secondary response to hapten-protein conjugates. I. Measurement of the effect with transferred cells and objections to the local environment hypothesis. Eur J Immunol. 1971 Jan;1(1):10–17. doi: 10.1002/eji.1830010103. [DOI] [PubMed] [Google Scholar]
  13. Schreiber R. D., Altman A., Katz D. H. Identification of a T cell hybridoma that produces large quantities of macrophage-activating factor. J Exp Med. 1982 Sep 1;156(3):677–689. doi: 10.1084/jem.156.3.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sredni B., Tse H. Y., Chen C., Schwartz R. H. Antigen-specific clones of proliferating T lymphocytes. I. Methodology, specificity, and MHC restriction. J Immunol. 1981 Jan;126(1):341–347. [PubMed] [Google Scholar]
  15. van Loveren H., Meade R., Askenase P. W. An early component of delayed-type hypersensitivity mediated by T cells and mast cells. J Exp Med. 1983 May 1;157(5):1604–1617. doi: 10.1084/jem.157.5.1604. [DOI] [PMC free article] [PubMed] [Google Scholar]

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