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. 1984 Apr;51(4):687–696.

Suppression of the anti-trimellityl (TM) IgE response in mice by conjugates of TM with polyvinyl alcohol.

B Y Wei, V Holford-Strevens, B G Carter, A H Sehon
PMCID: PMC1454556  PMID: 6706377

Abstract

The i.p. injection of 1 microgram of TM3-OA or TM9-OA with 1 mg of A1(OH)3 into B6D2F1 mice elicited the production of antibodies of the IgE and other classes to the trimellityl (TM) group and ovalbumin (OA). The induction of anti-TM antibodies belonging to the IgE and other immunoglobulin classes was specifically suppressed by the administration of tolerogenic conjugates prepared by coupling trimellitic anhydride (TMA) to the hydrophilic non-immunogenic polymer, polyvinyl alcohol (PVA), prior to immunization with TM-OA conjugates. More importantly, established anti-TM responses were also suppressed by these TM-PVA conjugates. By contrast, however, treatment with TM-PVA conjugates did not affect either the primary or the established anti-OA antibody response. The tolerogenic effects of the PVA conjugates were dose-dependent and appeared also to be dependent on the epitope density. Treatment with these conjugates also prevented immunized mice from showing any symptoms of systemic anaphylaxis on challenge with polyvalent TM-protein conjugates. These findings indicate that these conjugates may have the potential of useful therapeutic agents for the treatment of TMA-induced pulmonary hypersensitivity diseases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Herbert F. A., Orford R. Pulmonary hemorrhage and edema due to inhalation of resins containing tri-mellitic anhydride. Chest. 1979 Nov;76(5):546–551. doi: 10.1378/chest.76.5.546. [DOI] [PubMed] [Google Scholar]
  2. Holford-Strevens V., Lee W. Y., Kelly K. A., Sehon A. H. Suppression of IgE antibody production in sensitized mice and rats by tolerogenic conjugates of synthetic hydrophilic polymers with antigen or hapten: effect on antigen-induced histamine release from peritoneal mast cells. Int Arch Allergy Appl Immunol. 1982;67(2):109–116. doi: 10.1159/000232999. [DOI] [PubMed] [Google Scholar]
  3. Hubbard D. A., Lee W. Y., Sehon A. H. Suppression of the anti-DNP IgE response with tolerogenic conjugates of DNP with polyvinyl alcohol. I. Specific suppression of the anti-DNP IgE response. J Immunol. 1981 Feb;126(2):407–413. [PubMed] [Google Scholar]
  4. Katz D. H., Borel Y. Hapten-specific tolerance induced by hapten conjugates of D-glutamic acid, D-lysine (D-Gl) or isologous gamma-globulin: evidence for central B cell tolerance in the presence of carrier-primed helper T cells. J Immunol. 1978 Jun;120(6):1824–1827. [PubMed] [Google Scholar]
  5. Katz D. H., Strechschulte D. J., Benacerraf B. Inuction of immunological tolerance in immunoglobulin E B lymphocytes in rats. J Allergy Clin Immunol. 1975 Jun;55(6):403–410. doi: 10.1016/0091-6749(75)90079-2. [DOI] [PubMed] [Google Scholar]
  6. Kelly K. A., Lang G. M., Bundesen P. G., Holford-Strevens V., Böttcher I., Sehon A. H. The development of a radioallergosorbent test (RAST) for murine IgE antibodies. J Immunol Methods. 1980;39(4):317–333. doi: 10.1016/0022-1759(80)90232-x. [DOI] [PubMed] [Google Scholar]
  7. Lee W. Y., Hubbard D. A., Cripps V., Sehon A. H. Abrogation of the antibenzylpenicilloyl (BPO) IgE response with BPO-polyvinyl alcohol conjugates. Int Arch Allergy Appl Immunol. 1980;63(1):1–13. doi: 10.1159/000232604. [DOI] [PubMed] [Google Scholar]
  8. Lee W. Y., Sehon A. H. Suppression of reaginic antibody formation. I. Induction of hapten-specific tolerance. J Immunol. 1975 Feb;114(2 Pt 2):829–836. [PubMed] [Google Scholar]
  9. Lee W. Y., Sehon A. H. Suppression of the anti-DNP IgE response with tolerogenic conjugates of DNP with polyvinyl alcohol. II. Induction of hapten-specific suppressor T cells. J Immunol. 1981 Feb;126(2):414–418. [PubMed] [Google Scholar]
  10. Lee W. Y., Sehon A. H., von Specht B. U. Suppression of IgE antibodies to the (4-hydroxy-3-iodo-5-nitrophenyl)acetyl (NIP) group and induction of NIP-specific suppressor cells with NIP-poly-N-vinylpyrrolidone conjugates. Eur J Immunol. 1981 Jan;11(1):13–17. doi: 10.1002/eji.1830110104. [DOI] [PubMed] [Google Scholar]
  11. Liu F. T., Bargatze R. F., Katz D. H. Induction of immunologic tolerance to the trimellitate haptenic group in mice: model for a therapeutic approach to trimellitic anhydride-induced hypersensitivity syndromes in humans? J Allergy Clin Immunol. 1980 Oct;66(4):322–326. doi: 10.1016/0091-6749(80)90028-7. [DOI] [PubMed] [Google Scholar]
  12. Patterson R., Addington W., Banner A. S., Byron G. E., Franco M., Herbert F. A., Nicotra M. B., Pruzansky J. J., Rivera M., Roberts M. Antihapten antibodies in workers exposed to trimellitic anhydride fumes: a potential immunopathogenetic mechanism for the trimellitic anhydride pulmonary disease--anemia syndrome. Am Rev Respir Dis. 1979 Dec;120(6):1259–1267. doi: 10.1164/arrd.1979.120.6.1259. [DOI] [PubMed] [Google Scholar]
  13. Patterson R., Roberts M., Harris K. E., Levitz D., Zeiss C. R. Pulmonary and systemic immune responses of rhesus monkeys to intrabronchial administration of trimellitic anhydride. Clin Immunol Immunopathol. 1980 Mar;15(3):357–366. doi: 10.1016/0090-1229(80)90047-1. [DOI] [PubMed] [Google Scholar]
  14. Sale S. R., Roach D. E., Zeiss C. R., Patterson R. Clinical and immunologic correlations in trimellitic anhydride airway syndromes. J Allergy Clin Immunol. 1981 Sep;68(3):188–193. doi: 10.1016/0091-6749(81)90182-2. [DOI] [PubMed] [Google Scholar]
  15. Salvaggio J. E. Overview of occupational immunologic lung disease. J Allergy Clin Immunol. 1982 Jul;70(1):5–10. doi: 10.1016/0091-6749(82)90193-2. [DOI] [PubMed] [Google Scholar]
  16. Turner E. S., Pruzansky J. J., Patterson R., Zeiss C. R., Roberts M. Detection of antibodies in human serum using trimellityl-erythrocytes: direct and indirect haemagglutination and haemolysis. Clin Exp Immunol. 1980 Feb;39(2):470–476. [PMC free article] [PubMed] [Google Scholar]
  17. Zeiss C. R., Patterson R., Pruzansky J. J., Miller M. M., Rosenberg M., Levitz D. Trimellitic anhydride-induced airway syndromes: clinical and immunologic studies. J Allergy Clin Immunol. 1977 Aug;60(2):96–103. doi: 10.1016/0091-6749(77)90033-1. [DOI] [PubMed] [Google Scholar]

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