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. 1984 Apr;51(4):801–808.

Induction of immunoglobulin-secreting cells by 2-mercaptoethanol in in vitro culture of B cells from autoimmune mice.

M Fujiwara, A I Kariyone
PMCID: PMC1454562  PMID: 6231244

Abstract

Splenic B cells from older MRL/Mp-lpr/lpr (MRL/1) and male BXSB mice responded to 2-mercaptoethanol (2-ME) in in vitro culture and generated immunoglobulin-secreting cells (IgSC). Optimal concentration of 2-ME to induce IgSC was 5 X 10(-5) M. Kinetic studies revealed that the generation of IgSC was already apparent after 24 hr of culture and peak response was attained on the 2nd day. The response of B cells to 2 ME was enhanced in the presence of splenic T cells. Irradiation of B cells reduced the generation of IgSC. The B cell population of autoimmune mice responding to 2-ME to generate IgSC seems to be in a terminal stage of differentiation. This increased B cell differentiation was characteristic of autoimmune mice and assumed to have some significance in the development of autoimmune diseases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R. E., Warner N. L. Ionizing radiation and the immune response. Adv Immunol. 1976;24:215–335. doi: 10.1016/s0065-2776(08)60331-4. [DOI] [PubMed] [Google Scholar]
  2. Andrews B. S., Eisenberg R. A., Theofilopoulos A. N., Izui S., Wilson C. B., McConahey P. J., Murphy E. D., Roths J. B., Dixon F. J. Spontaneous murine lupus-like syndromes. Clinical and immunopathological manifestations in several strains. J Exp Med. 1978 Nov 1;148(5):1198–1215. doi: 10.1084/jem.148.5.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Claësson M. H., Flad H. D., Opitz H. G. B and T lymphocyte colony formation in agar: 2-mercaptoethanol-activated albumin can substitute for 2-mercaptoethanol. Cell Immunol. 1979 Sep 1;46(2):398–404. doi: 10.1016/0008-8749(79)90426-x. [DOI] [PubMed] [Google Scholar]
  4. Cohen P., Ziff M., Vitetta E. S. Characterization of a B cell defect in the NZB mouse manifested by an increased ratio of surface IgM to IgD. J Immunol. 1978 Sep;121(3):973–977. [PubMed] [Google Scholar]
  5. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  6. Eisenberg R. A., Izui S., McConahey P. J., Hang L., Peters C. J., Theofilopoulos A. N., Dixon F. J. Male determined accelerated autoimmune disease in BXSB mice: transfer by bone marrow and spleen cells. J Immunol. 1980 Sep;125(3):1032–1036. [PubMed] [Google Scholar]
  7. Fujiwara M., Akiyama Y. LPS-induced autoantibody response. I. Ontogenic development of PFC response to bromelain-treated syngeneic erythrocytes. Cell Immunol. 1980 Oct;55(2):366–380. doi: 10.1016/0008-8749(80)90168-9. [DOI] [PubMed] [Google Scholar]
  8. Fujiwara M., Kariyone A. Resistant nature of T cells of autoimmune mice to tolerance induction with human serum albumin. Immunology. 1982 Dec;47(4):573–579. [PMC free article] [PubMed] [Google Scholar]
  9. Fujiwara M., Katagiri T. Polyclonal antibody synthesis by bone marrow cells of New Zealand black mice. I. Assessment of activity in an adoptively transferred histocompatible host. Int Arch Allergy Appl Immunol. 1982;69(3):272–278. doi: 10.1159/000233183. [DOI] [PubMed] [Google Scholar]
  10. Goodman M. G., Fidler J. M., Weigle W. O. Nonspecific activation of murine lymphocytes. IV. Proliferation of a distinct, late maturing lymphocyte subpopulation induced by 2-mercaptoethanol. J Immunol. 1978 Nov;121(5):1905–1913. [PubMed] [Google Scholar]
  11. Goodman M. G., Weigle W. O. Nonspecific activation of murine lymphocytes. I. Proliferation and polyclonal activation induced by 2-mercaptoethanol and alpha-thioglycerol. J Exp Med. 1977 Mar 1;145(3):473–489. doi: 10.1084/jem.145.3.473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Igarashi T., Okada M., Kishimoto T., Yamamura Y. In vitro induction of polyclonal killer T cells with 2-mercaptoethanol and the essential role of macrophages in this process. J Immunol. 1977 May;118(5):1697–1703. [PubMed] [Google Scholar]
  13. Izui S., McConahey P. J., Dixon F. J. Increased spontaneous polyclonal activation of B lymphocytes in mice with spontaneous autoimmune disease. J Immunol. 1978 Dec;121(6):2213–2219. [PubMed] [Google Scholar]
  14. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  15. Laskin C. A., Smathers P. A., Reeves J. P., Steinberg A. D. Studies of defective tolerance induction in NZB mice. Evidence for a marrow pre-T cell defect. J Exp Med. 1982 Apr 1;155(4):1025–1036. doi: 10.1084/jem.155.4.1025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ly I. A., Mishell R. I. Separation of mouse spleen cells by passage through columns of sephadex G-10. J Immunol Methods. 1974 Aug;5(3):239–247. doi: 10.1016/0022-1759(74)90108-2. [DOI] [PubMed] [Google Scholar]
  17. Metcalf D. Role of mercaptoethanol and endotoxin in stimulating B lymphocyte colony formation in vitro. J Immunol. 1976 Mar;116(3):635–638. [PubMed] [Google Scholar]
  18. Moutsopoulos H. M., Boehm-Truitt M., Kassan S. S., Chused T. M. Demonstration of activation of B lymphocytes in New Zealand black mice at birth by an immunoradiometric assay for murine IgM. J Immunol. 1977 Nov;119(5):1639–1644. [PubMed] [Google Scholar]
  19. Muraguchi A., Kishimoto T., Miki Y., Kuritani T., Kaieda T., Yoshizaki K., Yamamura Y. T cell-replacing factor- (TRF) induced IgG secretion in a human B blastoid cell line and demonstration of acceptors for TRF. J Immunol. 1981 Aug;127(2):412–416. [PubMed] [Google Scholar]
  20. Sawada S., Talal N. Characteristics of in vitro production of antibodies to DNA in normal and autoimmune mice. J Immunol. 1979 Jun;122(6):2309–2313. [PubMed] [Google Scholar]
  21. Steward M. W., Hay F. C. Changes in immunoglobulin class and subclass of anti-DNA antibodies with increasing age in N/ZBW F1 hybrid mice. Clin Exp Immunol. 1976 Nov;26(2):363–370. [PMC free article] [PubMed] [Google Scholar]
  22. Theofilopoulos A. N., Dixon F. J. Etiopathogenesis of murine SLE. Immunol Rev. 1981;55:179–216. doi: 10.1111/j.1600-065x.1981.tb00343.x. [DOI] [PubMed] [Google Scholar]
  23. Theofilopoulos A. N., Eisenberg R. A., Bourdon M., Crowell J. S., Jr, Dixon F. J. Distribution of lymphocytes identified by surface markers in murine strains with systemic lupus erythematosus-like syndromes. J Exp Med. 1979 Feb 1;149(2):516–534. doi: 10.1084/jem.149.2.516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Theofilopoulos A. N., Shawler D. L., Eisenberg R. A., Dixon F. J. Splenic immunoglobulin-secreting cells and their regulation in autoimmune mice. J Exp Med. 1980 Feb 1;151(2):446–466. doi: 10.1084/jem.151.2.446. [DOI] [PMC free article] [PubMed] [Google Scholar]

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