Abstract
The systemic secretion of rat mucosal mast cell protease (RMCPII) was examined in Nippostrongylus-primed rats injected intravenously with N. brasiliensis whole worm antigen. The secretory response in primed rats was both time- and dose-dependent whereas no RMCPII was present in the sera of naive rats challenged with antigen. RMCPII was detected in the sol and gel phases of intestinal perfusates, the release of enzyme into the gut lumen occurring very rapidly after challenge in immune rats. Mucosal permeability, assessed by measuring the passage of Evan's blue from the blood into the gut lumen was both time- and dose-dependent and reflected the combined capillary and epithelial permeability. Although the release of RMCPII into the gut lumen occurred more rapidly than the intraluminal accumulation of Evan's blue, these two events were highly correlated. There were, in addition significant correlations between the systemic and enteric secretion of RMCPII and the enteric accumulation of Evan's blue. These results indicate that RMCPII may have a role in altering intestinal mucosal permeability during systemic anaphylaxis in the rat.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Miller H. R., Woodbury R. G., Huntley J. F., Newlands G. Systemic release of mucosal mast-cell protease in primed rats challenged with Nippostrongylus brasiliensis. Immunology. 1983 Jul;49(3):471–479. [PMC free article] [PubMed] [Google Scholar]
- Nawa Y. Increased permeability of gut mucosa in rats infected with Nippostrongylus brasiliensis. Int J Parasitol. 1979 Jun;9(3):251–255. doi: 10.1016/0020-7519(79)90036-5. [DOI] [PubMed] [Google Scholar]
- Nawa Y., Miller H. R., Hall E., Jarrett E. E. Adoptive transfer of total and parasite-specific IgE responses in rats infected with Nippostrongylus brasiliensis. Immunology. 1981 Sep;44(1):119–123. [PMC free article] [PubMed] [Google Scholar]
- Nawa Y., Miller H. R. Protection against Nippostrongylus brasiliensis by adoptive immunization with immune thoracic duct lymphocytes. Cell Immunol. 1978 Apr;37(1):51–60. doi: 10.1016/0008-8749(78)90173-9. [DOI] [PubMed] [Google Scholar]
- Ogilvie B. M., Love R. J. Co-operation between antibodies and cells in immunity to a nematode parasite. Transplant Rev. 1974;19(0):147–169. doi: 10.1111/j.1600-065x.1974.tb00131.x. [DOI] [PubMed] [Google Scholar]
- Sage H., Woodbury R. G., Bornstein P. Structural studies on human type IV collagen. J Biol Chem. 1979 Oct 10;254(19):9893–9900. [PubMed] [Google Scholar]
- Urquhart G. M., Mulligan W., Eadie R. M., Jennings F. W. Immunological studies on Nippostrongylus brasiliensis infection in the rat: the role of local anaphylaxis. Exp Parasitol. 1965 Oct;17(2):210–217. doi: 10.1016/0014-4894(65)90023-8. [DOI] [PubMed] [Google Scholar]
- Woodbury R. G., Gruzenski G. M., Lagunoff D. Immunofluorescent localization of a serine protease in rat small intestine. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2785–2789. doi: 10.1073/pnas.75.6.2785. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodbury R. G., Katunuma N., Kobayashi K., Titani K., Neurath H., Anderson W. F., Matthews B. W. Covalent structure of a group-specific protease from rat small intestine. Appendix: crystallographic data for a group specific protease from rat intestine. Biochemistry. 1978 Mar 7;17(5):811–819. doi: 10.1021/bi00598a010. [DOI] [PubMed] [Google Scholar]
- Woodbury R. G., Miller H. R. Quantitative analysis of mucosal mast cell protease in the intestines of Nippostrongylus-infected rats. Immunology. 1982 Jul;46(3):487–495. [PMC free article] [PubMed] [Google Scholar]
- Woodbury R. G., Neurath H. Purification of an atypical mast cell protease and its levels in developing rats. Biochemistry. 1978 Oct 3;17(20):4298–4304. doi: 10.1021/bi00613a029. [DOI] [PubMed] [Google Scholar]