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. 1984 May;52(1):97–105.

The control of antibody diversity during IgM and IgG anti-sheep red cell responses in mice.

M P Brittle, N J Bradley, K J Gomer, J H Playfair, A J Davies
PMCID: PMC1454569  PMID: 6232209

Abstract

The isoelectric heterogeneity of both IgM and IgG murine anti-sheep red cell serum antibodies has been examined using an adaptation of published methods. It was found that the IgM spectra were restricted in a characteristic manner, implying oligoclonality. In cell transfer experiments, the T cell dependency of the IgM responses was confirmed. Further, helper cells appeared to switch on IgG antibody production and, simultaneously, recruit many novel IgG-forming clones into the response. The pattern of the IgM oligoclonality was attributed in part to the inheritance of genes at, or closely linked to, the Igl heavy chain locus. These findings are discussed in relation to current research on the regulation of diversity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brittle M. P., Playfair J. H. The role of T cells in regulating immunoglobulin class and subclass. Immunology. 1980 Nov;41(3):743–752. [PMC free article] [PubMed] [Google Scholar]
  2. Conger J. D., Lewis G. K., Goodman J. W. Idiotype profile of an immune response. I. Contrasts in idiotypic dominance between primary and secondary responses and between IgM and IgG plaque-forming cells. J Exp Med. 1981 May 1;153(5):1173–1186. doi: 10.1084/jem.153.5.1173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davies A. J., Leuchars E., Wallis V., Koller P. C. The mitotic response of thymus-derived cells to antigenic stimulus. Transplantation. 1966 Jul;4(4):438–451. doi: 10.1097/00007890-196607000-00008. [DOI] [PubMed] [Google Scholar]
  4. Mage M. G., McHugh L. L., Rothstein T. L. Mouse lymphocytes with and without surface immunoglobulin: preparative scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin. J Immunol Methods. 1977;15(1):47–56. doi: 10.1016/0022-1759(77)90016-3. [DOI] [PubMed] [Google Scholar]
  5. Mäkelä O., Karjalainen K. Inherited immunoglobulin idiotypes of the mouse. Immunol Rev. 1977;34:119–138. doi: 10.1111/j.1600-065x.1977.tb00370.x. [DOI] [PubMed] [Google Scholar]
  6. Nicolotti R. A., Briles D. E., Schroer J. A., Davie J. M. Isoelectric focusing of immunoglobulins: improved methodology. J Immunol Methods. 1980;33(2):101–115. doi: 10.1016/s0022-1759(80)80001-9. [DOI] [PubMed] [Google Scholar]
  7. Phillips J. M., Dresser D. W. Isoelectric spectra of different classes of anti-erythrocyte antibodies. Eur J Immunol. 1973 Aug;3(8):524–527. doi: 10.1002/eji.1830030816. [DOI] [PubMed] [Google Scholar]
  8. Pink J. R., Askonas B. A. Diversity of antibodies to cross-reacting nitrophenyl haptens in inbred mice. Eur J Immunol. 1974 Jun;4(6):426–430. doi: 10.1002/eji.1830040608. [DOI] [PubMed] [Google Scholar]

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