Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1984 May;52(1):41–48.

Effect of feeding a diet with half of the recommended levels of all vitamins on the natural and inducible levels of cytotoxic activity in mouse spleen cells.

Q B Saxena, R K Saxena, W H Adler
PMCID: PMC1454579  PMID: 6232207

Abstract

Groups of 6-week-old female C57Bl/6 mice were fed a normal diet with recommended levels of all vitamins or a vitamin-deficient (VD) diet containing half of the recommended level of each vitamin. At different time periods (1-11 weeks) after the initiation of diets, basal natural killer (NK) activity, interleukin-2 (IL-2) and concanavalin A (Con A)-induced cytotoxic activity, Con A-induced IL-2 production and levels of allospecific cytotoxic T cell activity generated in a mixed lymphocyte culture (MLC), were studied in spleen cells derived from control and VD mice. Results indicated that: (i) spleen NK activity remained normal until 2 weeks after the initiation of VD diet, fell steeply to low levels at the 4 and 5 week time points and remained depressed thereafter; (ii) IL-2- and Con A-induced levels of cytotoxic activity in spleen cells derived from VD mice declined at 4 weeks after the institution of VD diet, and then remained low throughout the study; (iii) the capacity of spleen cells from VD mice to generate IL-2 in response to Con A and cytotoxic T cells in response to allogeneic spleen cells, was normal at 1 and 4 weeks after initiation of the VD diet and was markedly depressed at the 6 and 9 week time points. These results suggest that partial combined deficiencies of dietary vitamins strongly influence assays of immune function.

Full text

PDF
41

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowman B. B., Rosenberg I. H. Assessment of the nutritional status of the elderly. Am J Clin Nutr. 1982 May;35(5 Suppl):1142–1151. doi: 10.1093/ajcn/35.5.1142. [DOI] [PubMed] [Google Scholar]
  2. Gillis S., Ferm M. M., Ou W., Smith K. A. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed] [Google Scholar]
  3. Gillis S., Smith K. A., Watson J. Biochemical characterization of lymphocyte regulatory molecules. II. Purification of a class of rat and human lymphokines. J Immunol. 1980 Apr;124(4):1954–1962. [PubMed] [Google Scholar]
  4. Harrill I., Cervone N. Vitamin status of older women. Am J Clin Nutr. 1977 Mar;30(3):431–440. doi: 10.1093/ajcn/30.3.431. [DOI] [PubMed] [Google Scholar]
  5. Keusch G. T. Host defense mechanisms in protein energy malnutrition. Adv Exp Med Biol. 1981;135:183–209. doi: 10.1007/978-1-4615-9200-6_10. [DOI] [PubMed] [Google Scholar]
  6. Kiessling R., Petranyi G., Kärre K., Jondal M., Tracey D., Wigzell H. Killer cells: a functional comparison between natural, immune T-cell and antibody-dependent in vitro systems. J Exp Med. 1976 Apr 1;143(4):772–780. doi: 10.1084/jem.143.4.772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mezey E. Medical problems associated with alcoholism. Prim Care. 1974 Jun;1(2):293–316. [PubMed] [Google Scholar]
  8. Saxena Q. B., Saxena R. K., Adler W. H. Effect of protein calorie malnutrition on the levels of natural and inducible cytotoxic activities in mouse spleen cells. Immunology. 1984 Apr;51(4):727–733. [PMC free article] [PubMed] [Google Scholar]
  9. Saxena Q. B., Saxena R. K., Adler W. H. Regulation of natural killer activity in vivo. III. Effect of hypophysectomy and growth hormone treatment on the natural killer activity of the mouse spleen cell population. Int Arch Allergy Appl Immunol. 1982;67(2):169–174. [PubMed] [Google Scholar]
  10. Saxena R. K., Adler W. H. Modulation of natural cytotoxicity by alloantibodies. I. Alloantisera enhancement of cytotoxicity of mouse spleen cells toward a human myeloid cell line. J Immunol. 1979 Aug;123(2):846–851. [PubMed] [Google Scholar]
  11. Saxena R. K., Adler W. H., Nordin A. A. Modulation of natural cytotoxicity by alloantibodies. IV. A comparative study of the activation of mouse spleen cell cytotoxicity by anti H-2 antisera, interferon, and mitogens. Cell Immunol. 1981 Sep 1;63(1):28–41. doi: 10.1016/0008-8749(81)90026-5. [DOI] [PubMed] [Google Scholar]
  12. Saxena R. K., Saxena Q. B., Adler W. H. Defective T-cell response in beige mutant mice. Nature. 1982 Jan 21;295(5846):240–241. doi: 10.1038/295240a0. [DOI] [PubMed] [Google Scholar]
  13. Saxena R. K., Saxena Q. B., Adler W. H. Identity of effector cells participating in the reverse antibody-dependent cell-mediated cytotoxicity. Immunology. 1982 Jun;46(2):459–464. [PMC free article] [PubMed] [Google Scholar]
  14. Saxena R. K., Saxena Q. B., Collins G. D., Adler W. H. Augmentation of spleen natural killer activity in mice treated with interleukin-2 preparation. Indian J Exp Biol. 1983 Feb;21(2):54–58. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES