Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1984 May;52(1):25–30.

Accessory cells distinct from macrophages in lymphocyte proliferation of cynomolgus monkeys.

M Tatsumi, Y Imamura
PMCID: PMC1454580  PMID: 6609122

Abstract

Mononuclear cells of cynomolgus monkeys were separated into four distinct fractions based on their buoyant density on discontinuous gradients of Percoll. Cells of the lightest density (fraction I) were enriched for macrophages and B cells, whereas cells from the denser fractions (fractions II-IV) consisted mainly of T cells, especially in fraction IV up to 88%. Cells of moderate density (fractions II and III) could respond better to T cell mitogens than unfractionated cells. In contrast, cells of fraction I not only failed to respond to mitogens, but also exerted an inhibitory effect on lymphocyte proliferation, indicating that macrophages might suppress the response in cynomolgus monkeys. Cells alone having the heaviest density (fraction IV), although enriched for T cells, also showed no significant response. However, addition of mitomycin C-treated fraction II or III resulted in partial restoration in the proliferative response of this fraction. Further examination revealed that the accessory activity resided in the non-adherent, nonphagocytic, non-E rosette-forming and Fc receptor-negative cells with moderate density, suggesting the presence of accessory cells distinct from monocyte/macrophage lineage in cynomolgus monkeys.

Full text

PDF
25

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Canonica G. W., Kabelitz D., Sjöberg O., Aigrain Y., Wigzell H. Density distribution profiles of T cells: TM, TG and TA cells and response patterns in autologous versus allogeneic MLRs. Scand J Immunol. 1982 Sep;16(3):243–248. doi: 10.1111/j.1365-3083.1982.tb00719.x. [DOI] [PubMed] [Google Scholar]
  2. Folch H., Yoshinaga M., Waksman B. H. Regulation of lymphocyte responses in vitro. 3. Inhibition by adherent cells of the T-lymphocyte response to phytohemagglutinin. J Immunol. 1973 Mar;110(3):835–839. [PubMed] [Google Scholar]
  3. Fraser B. A., Thunberg A. L., Kindt T. J. Variable region correlates of group b allotypes: amino acid sequence studies of b9 L chains from homogeneous antibodies. Eur J Immunol. 1978 Jun;8(6):380–385. doi: 10.1002/eji.1830080603. [DOI] [PubMed] [Google Scholar]
  4. Gutierrez C., Bernabe R. R., Vega J., Kreisler M. Purification of human T and B cells by a discontinuous density gradient of percoll. J Immunol Methods. 1979;29(1):57–63. doi: 10.1016/0022-1759(79)90125-x. [DOI] [PubMed] [Google Scholar]
  5. Hirschberg H., Braathen L. R., Thorsby E. Antigen presentation by vascular endothelial cells and epidermal Langerhans cells: the role of HLA-DR. Immunol Rev. 1982;66:57–77. doi: 10.1111/j.1600-065x.1982.tb00434.x. [DOI] [PubMed] [Google Scholar]
  6. Holt P. G., Warner L. A., Mayrhofer G. Macrophages as effectors of T suppression: T-lymphocyte-dependent macrophage-mediated suppression of mitogen-induced blastogenesis in the rat. Cell Immunol. 1981 Sep 1;63(1):57–70. doi: 10.1016/0008-8749(81)90028-9. [DOI] [PubMed] [Google Scholar]
  7. Kaplan M. E., Clark C. An improved rosetting assay for detection of human T lymphocytes. J Immunol Methods. 1974 Jul;5(2):131–135. doi: 10.1016/0022-1759(74)90003-9. [DOI] [PubMed] [Google Scholar]
  8. Klinkert W. E., LaBadie J. H., O'Brien J. P., Beyer C. F., Bowers W. E. Rat dendritic cells function as accessory cells and control the production of a soluble factor required for mitogenic responses of T lymphocytes. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5414–5418. doi: 10.1073/pnas.77.9.5414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kurnick J. T., Ostberg L., Stegagno M., Kimura A. K., Orn A., Sjöberg O. A rapid method for the separation of functional lymphoid cell populations of human and animal origin on PVP-silica (Percoll) density gradients. Scand J Immunol. 1979;10(6):563–573. doi: 10.1111/j.1365-3083.1979.tb01391.x. [DOI] [PubMed] [Google Scholar]
  10. Rosenstreich D. L., Farrar J. J., Dougherty S. Absolute macrophage dependency of T lymphocyte activation by mitogens. J Immunol. 1976 Jan;116(1):131–139. [PubMed] [Google Scholar]
  11. Schmidtke J. R., Hatfield S. Activation of purified human thymus-derived (T) cells by mitogens. II. Monocyte- macrophage potentiation of mitogen-induced DNA synthesis. J Immunol. 1976 Feb;116(2):357–362. [PubMed] [Google Scholar]
  12. Steinman R. M., Nussenzweig M. C. Dendritic cells: features and functions. Immunol Rev. 1980;53:127–147. doi: 10.1111/j.1600-065x.1980.tb01042.x. [DOI] [PubMed] [Google Scholar]
  13. Stingl G., Katz S. I., Clement L., Green I., Shevach E. M. Immunologic functions of Ia-bearing epidermal Langerhans cells. J Immunol. 1978 Nov;121(5):2005–2013. [PubMed] [Google Scholar]
  14. Stout R. D., Fisher M. Suppression of lymphocyte proliferative responses: characterization of the suppressor and kinetics of suppression. J Immunol. 1983 Apr;130(4):1573–1579. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES