Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1984 May;52(1):7–15.

The flow and composition of lymph from the caudal mediastinal lymph node of sheep.

J Spencer, J G Hall
PMCID: PMC1454582  PMID: 6715022

Abstract

By cannulating the efferent duct of the caudal mediastinal lymph node in sheep, lymph from the lower respiratory tract was collected under physiological conditions for several days. In 18 such preparations the flow rate varied from 4 to 12 ml/hr between individuals and the lymphocyte count between 4000 and 117,000/mm3. The protein content of the lymph plasma averaged nearly 60% of that of the blood, and this indication of the high permeability of the capillary bed of the lungs was confirmed by measuring the time taken for intravenous doses of 125I-albumin to equilibrate between the blood and mediastinal lymph plasma. The concentration of immunoglobulin A was higher in the mediastinal lymph than in blood serum, while the reverse was true of the concentrations of IgG1, IgG2, and IgM. This evidence for the local production of IgA by the intra thoracic lymphoid tissue was supported by the demonstration by immunoperoxidase techniques of IgA-containing plasma cells in sections cut from the caudal mediastinal nodes, and of IgA-containing immunoblasts in the lymph.

Full text

PDF
7

Images in this article

12Figure 3
on p.12

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alley M. R., Wells P. W., Smith W. D., Gardiner A. C. The distribution of immunoglobulin in the respiratory tract of sheep. Vet Pathol. 1980 May;17(3):372–380. doi: 10.1177/030098588001700311. [DOI] [PubMed] [Google Scholar]
  2. Bice D. E., Harris D. L., Muggenburg B. A. Regional immunologic responses following localized deposition of antigen in the lung. Exp Lung Res. 1980 Mar;1(1):33–41. doi: 10.3109/01902148009057511. [DOI] [PubMed] [Google Scholar]
  3. Bienenstock J., Johnston N., Perey D. Y. Bronchial lymphoid tissue. I. Morphologic characteristics. Lab Invest. 1973 Jun;28(6):686–692. [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. Glover D. J., Hall J. G. A method for the collection of lymph from the prescapular lymph node of unanaethetized sheep. Lab Anim. 1976 Oct;10(10):403–408. doi: 10.1258/002367776780956953. [DOI] [PubMed] [Google Scholar]
  6. Gorin A. B., Gould J. Immunoglobulin synthesis in the lungs and caudal mediastinal lymph node of sheep. J Immunol. 1979 Sep;123(3):1339–1342. [PubMed] [Google Scholar]
  7. HALL J. G., MORRIS B. The lymph-borne cells of the immune response. Q J Exp Physiol Cogn Med Sci. 1963 Jul;48:235–247. doi: 10.1113/expphysiol.1963.sp001660. [DOI] [PubMed] [Google Scholar]
  8. HEATH T. J., LASCELLES A. K., MORRIS B. The cells of sheep lymph. J Anat. 1962 Jul;96:397–408. [PMC free article] [PubMed] [Google Scholar]
  9. Hall J. G. A method for collecting lymph from the prefemoral lymph node of unanaesthetised sheep. Q J Exp Physiol Cogn Med Sci. 1967 Apr;52(2):200–205. doi: 10.1113/expphysiol.1967.sp001902. [DOI] [PubMed] [Google Scholar]
  10. Hall J. G., Hopkins J., Orlans E. Studies on the lymphocytes of sheep. III. Destination of lymph-borne immunoblasts in relation to their tissue of origin. Eur J Immunol. 1977 Jan;7(1):30–37. doi: 10.1002/eji.1830070108. [DOI] [PubMed] [Google Scholar]
  11. Hall J. G., Smith M. E., Edwards P. A., Shooter K. V. The low concentration of macroglobulin antibodies in peripheral lymph. Immunology. 1969 Jun;16(6):773–778. [PMC free article] [PubMed] [Google Scholar]
  12. Hall J. Lymphocyte recirculation and the gut: the cellular basis of humoral immunity in the intestine. Blood Cells. 1979 Aug;5(3):479–497. [PubMed] [Google Scholar]
  13. Joel D. D., Chanana A. D., Chandra P. Immune responses in pulmonary lymph of sheep after intrabronchial administration of heterologous erythrocytes. Am Rev Respir Dis. 1980 Dec;122(6):925–932. doi: 10.1164/arrd.1980.122.6.925. [DOI] [PubMed] [Google Scholar]
  14. Kaltreider H. B., Kyselka L., Salmon S. E. Immunology of the lower respiratory tract. II. The plaque-forming response of canine lymphoid tissues to sheep erythrocytes after intrapulmonary or intravenous immunization. J Clin Invest. 1974 Aug;54(2):263–270. doi: 10.1172/JCI107761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LASCELLES A. K., MORRIS B. Surgical techniques for the collection of lymph from unanaesthetized sheep. Q J Exp Physiol Cogn Med Sci. 1961 Jul;46:199–205. doi: 10.1113/expphysiol.1961.sp001536. [DOI] [PubMed] [Google Scholar]
  16. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  17. Staub N. C., Bland R. D., Brigham K. L., Demling R., Erdmann A. J., 3rd, Woolverton W. C. Preparation of chronic lung lymph fistulas in sheep. J Surg Res. 1975 Nov;19(5):315–320. doi: 10.1016/0022-4804(75)90056-6. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES