Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1984 May;52(1):87–96.

Hapten-specific B cell blockade of the immune response to a thymus-independent-1 antigen produced by concomitant administration of a thymus-independent-2 antigen.

H Snippe, A J Van Houte, J K Inman, E F Lizzio, B Merchant
PMCID: PMC1454594  PMID: 6201438

Abstract

CBA/N mice harbour an X-linked B cell defect which is transmitted by CBA/N female mice to their hybrid male progeny. These mice mount normal responses to thymus-dependent (TD) and some thymus-independent (TI-1) antigens, while the response to TI-2 antigens is absent. Hapten-specific plaque-forming cell (PFC) responses to TD antigens can be blockaded by concomitant exposure of these mice to TI-2 antigens bearing the same hapten. This paper investigates in defective mice the blockade of their response to TNP3-LPS (trinitrophenylated lipopolysaccharide, a TI-1 antigen), imposed by DNP59-Ficoll (dinitrophenylated Ficoll, a TI-2 antigen). The effectiveness of the blocking agent, DNP59-Ficoll, differed in various inbred mouse strains: CBA/N X C3H/HeN F1 male greater than CBA/N female greater than CBA/N X C3H/HeN F1 female. The role of T cells in the observed hapten-specific blockade phenomenon was investigated using athymic CBA/N nude mice and a B cell tolerogen. Our findings indicate that T cell participation is not essential for the blockade of CBA/N PFC responses and they suggest that direct blockade of TI- and TD-responsive B cell populations is likely to occur.

Full text

PDF
87

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amsbaugh D. F., Hansen C. T., Prescott B., Stashak P. W., Barthold D. R., Baker P. J. Genetic control of the antibody response to type 3 pneumococcal polysaccharide in mice. I. Evidence that an X-linked gene plays a decisive role in determining responsiveness. J Exp Med. 1972 Oct 1;136(4):931–949. doi: 10.1084/jem.136.4.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohen P. L., Scher I., Mosier D. E. In vitro studies of the genetically determined unresponsiveness to thymus-independent antigens in CBA/N mice. J Immunol. 1976 Feb;116(2):301–304. [PubMed] [Google Scholar]
  3. Fernandez C., Möller G. Immunological unresponsiveness to thymus-independent antigens: two fundamentally different genetic mechanisms of B-cell unresponsiveness to dextran. J Exp Med. 1977 Dec 1;146(6):1663–1677. doi: 10.1084/jem.146.6.1663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Humphrey J. H. Tolerogenic or immunogenic activity of hapten-conjugated polysaccharides correlated with cellular localization. Eur J Immunol. 1981 Mar;11(3):212–220. doi: 10.1002/eji.1830110310. [DOI] [PubMed] [Google Scholar]
  5. Inman J. K., Merchant B., Claflin L., Tacey S. E. Coupling of large haptens to proteins and cell surfaces: preparation of stable, optimally sensitized erythrocytes for hapten-specific, hemolytic plaque assays. Immunochemistry. 1973 Mar;10(3):165–174. doi: 10.1016/0019-2791(73)90005-0. [DOI] [PubMed] [Google Scholar]
  6. Inman J. K., Merchant B., Tacey S. E. Synthesis of large haptenic compounds having a common functional group that permits covalent linkage to proteins, cell surfaces, and adsorbents. Immunochemistry. 1973 Mar;10(3):153–163. doi: 10.1016/0019-2791(73)90004-9. [DOI] [PubMed] [Google Scholar]
  7. Inman J. K. Thymus-independent antigens: the preparation of covalent, hapten-ficoll conjugates. J Immunol. 1975 Feb;114(2 Pt 1):704–709. [PubMed] [Google Scholar]
  8. Jacobs D. M. Structural and genetic basis of the in vivo immune response to TNP-LPS. J Immunol. 1975 Oct;115(4):988–992. [PubMed] [Google Scholar]
  9. Klaus G. G. B cell tolerance induced by polymeric antigens. II. Effects of tolerance on hapten-binding lymphocyte levels in primary and secondary antibody responses. Eur J Immunol. 1976 Jun;5(6):366–372. doi: 10.1002/eji.1830050603. [DOI] [PubMed] [Google Scholar]
  10. Klaus G. G., Humphrey J. H. B cell tolerance induced by polymeric antigens. I. Comparison of the dose and epitope density requirements for inactivation of primed and unprimed B cells in vivo. Eur J Immunol. 1976 Jun;5(6):361–365. doi: 10.1002/eji.1830050602. [DOI] [PubMed] [Google Scholar]
  11. Klaus G. G., Willcox H. N. B cell tolerance induced by polymeric antigens. III. Dissociation of antibody formation and memory generation in tolerant mice. Eur J Immunol. 1975 Oct;5(10):699–704. doi: 10.1002/eji.1830051010. [DOI] [PubMed] [Google Scholar]
  12. McIntire F. C., Sievert H. W., Barlow G. H., Finley R. A., Lee A. Y. Chemical, physical, biological properties of a lipopolysaccharide from Escherichia coli K-235. Biochemistry. 1967 Aug;6(8):2363–2372. doi: 10.1021/bi00860a011. [DOI] [PubMed] [Google Scholar]
  13. Merchant B., Inman J. K. Hapten-specific hemolytic plaque assays usually fail to detect most of the diversity in the anti-hapten response. J Exp Med. 1977 Feb 1;145(2):372–389. doi: 10.1084/jem.145.2.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Merchant B., Snippe H., Lizzio E. F., Inman J. K. Cellular and molecular requirements for X-linked, hapten-specific B-cell blockade in CBA/N mice. J Exp Med. 1978 Jun 1;147(6):1755–1770. doi: 10.1084/jem.147.6.1755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Merchant B., Snippe H., Lizzio E. F., Inman J. K. X-linked genetic control of hapten-polysaccharide-mediated specific immune unresponsiveness in CBA/N mice. J Immunol. 1978 Apr;120(4):1362–1368. [PubMed] [Google Scholar]
  16. Mond J. J., Scher I., Mosier D. E., Baese M., Paul W. E. T-independent responses in B cell-defective CBA/N mice to Brucella abortus and to trinitrophenyl (TNP) conjugates of Brucella abortus. Eur J Immunol. 1978 Jul;8(7):459–463. doi: 10.1002/eji.1830080703. [DOI] [PubMed] [Google Scholar]
  17. Mosier D. E., Scher I., Paul W. E. In vitro responses of CBA/N mice: spleen cells of mice with an X-linked defect that precludes immune responses to several thymus-independent antigens can respond to TNP-lipopolysaccharide. J Immunol. 1976 Oct;117(4):1363–1369. [PubMed] [Google Scholar]
  18. Mosier D. E., Zitron I. M., Mond J. J., Ahmed A., Scher I., Paul W. E. Surface immunoglobulin D as a functional receptor for a subclass of B lymphocytes. Immunol Rev. 1977;37:89–104. doi: 10.1111/j.1600-065x.1977.tb00246.x. [DOI] [PubMed] [Google Scholar]
  19. Scher I., Ahmed A., Strong D. M., Steinberg A. D., Paul W. E. X-linked B-lymphocyte immune defect in CBA/HN mice. I. Studies of the function and composition of spleen cells. J Exp Med. 1975 Apr 1;141(4):788–803. [PMC free article] [PubMed] [Google Scholar]
  20. Scher I., Sharrow S. O., Paul W. E. X-linked B-lymphocyte defect in CBA/N mice. III. Abnormal development of B-lymphocyte populations defined by their density of surface immunoglobulin. J Exp Med. 1976 Aug 1;144(2):507–518. doi: 10.1084/jem.144.2.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Snippe H., Van Houte A. J., Janssen A., Lizzio E. F., Willers J. M., Merchant B. Characterization of immunogenic properties of haptenated liposomal model membranes in mice. VII. Synergistic responses to haptenated liposomes and haptenated thymus-dependent antigens in CBA/N mice. Immunology. 1982 Apr;45(4):613–620. [PMC free article] [PubMed] [Google Scholar]
  22. Snippe H., van Houte A. J., Lizzio E. F., Willers J. M., Merchant B. Characterization of immunogenic properties of haptenated liposomal model membranes in mice. VI. Response in B-cell-defective CBA/N mice. Immunology. 1982 Mar;45(3):545–551. [PMC free article] [PubMed] [Google Scholar]
  23. Subbarao B., Mosier D. E. Lyb antigens and their role in B lymphocyte activation. Immunol Rev. 1982;69:81–97. doi: 10.1111/j.1600-065x.1983.tb00450.x. [DOI] [PubMed] [Google Scholar]
  24. Vogel S. N., Hansen C. T., Rosenstreich D. L. Characterization of a congenitally LPS-resistant, athymic mouse strain. J Immunol. 1979 Feb;122(2):619–622. [PubMed] [Google Scholar]
  25. van Houte A. J., Snippe H., Willers J. M. Characterization of immunogenic properties of haptenated liposomal model membranes in mice. I. Thymus independence of the antigen. Immunology. 1979 Jun;37(2):505–514. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES