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. 1984 May;52(1):17–24.

Facb rosette-forming cells in mice: studies on their functional significance.

L J Eales, N D Hall, I M Hunneyball
PMCID: PMC1454599  PMID: 6201437

Abstract

Lymphocytes bearing receptors for the Facb fragment of IgG have been shown previously to be elevated in the peripheral blood of patients with rheumatoid arthritis. The generation of these cells and their possible functional role in immune regulation have been investigated in mice. Facb rosette-forming (Facb-R+) lymphocytes were found to be elevated in the spleens of mice mounting a secondary plaque-forming cell (PFC) response to sheep erythrocytes but not during the primary response. Splenic Facb-R+ lymphocytes were also elevated when a cross-reacting antigen (goat erythrocytes) was used for the secondary immunization but not when a non-cross-reacting antigen (chicken erythrocytes) was used. Both primary and secondary immunization with bacterial lipopolysaccharide resulted in elevation of splenic Facb-R+ lymphocytes. Administration of antigen-specific Facb fragment in conjunction with antigen (calf erythrocytes) produced a suppression of the secondary PFC response. However, F(ab')2 fragments produced no such effect. This suppressive effect was shown to be antigen-specific since administration of Facb fragment of anti-calf erythrocyte IgG had no suppressive effect on the secondary PFC response to sheep erythrocytes. No change in splenic Facb-R+ lymphocytes was observed during delayed hypersensitivity responses to either sheep erythrocytes or the contact-sensitizing agent oxazolone. These results indicate that Facb-R+ lymphocytes are generated during secondary humoral responses but not cell-mediated immune responses, and suggest that these cells may exert a suppressive influence on antibody production. These findings are discussed in relation to the occurrence of these cells in patients with rheumatoid arthritis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caraux J., Klein B., Thierry C., Serrou B. Amplification of the polyclonal activation of human T cells. I. Null-cell products promote the polyclonal proliferation of T cells. Immunology. 1982 Feb;45(2):257–263. [PMC free article] [PubMed] [Google Scholar]
  2. Carvalho E. M., Horwitz D. A. Characterization of a non-T, non-B human blood lymphocyte that mediates the enhancing effects of immune complexes on lymphocyte blastogenesis. J Immunol. 1980 Apr;124(4):1656–1661. [PubMed] [Google Scholar]
  3. Chan P. L., Sinclair N. R. Regulation of the immune response. V. An analysis of the function of the Fc portion of antibody in suppression of an immune response with respect to interaction with components of the lymphoid system. Immunology. 1971 Dec;21(6):967–981. [PMC free article] [PubMed] [Google Scholar]
  4. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  5. Hall N. D., Winrow V. R., Bacon P. A. Lymphocytes bearing Fc gamma receptors in rheumatoid arthritis. I. An increased subpopulation of cells in rheumatoid arthritis detected with Facb rosettes. Ann Rheum Dis. 1980 Dec;39(6):554–558. doi: 10.1136/ard.39.6.554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hjelm H., Hjelm K., Sjöquist J. Protein A from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins. FEBS Lett. 1972 Nov 15;28(1):73–76. doi: 10.1016/0014-5793(72)80680-x. [DOI] [PubMed] [Google Scholar]
  7. Hoffmann M. K. Antibody regulates the cooperation of B cells with helper cells. Immunol Rev. 1980;49:79–91. doi: 10.1111/j.1600-065x.1980.tb00427.x. [DOI] [PubMed] [Google Scholar]
  8. Horwitz D. A., Garrett M. A. Distinctive functional properties of human blood L lymphocytes: a comparison with T lymphocytes, B lymphocytes, and monocytes. J Immunol. 1977 May;118(5):1712–1721. [PubMed] [Google Scholar]
  9. Horwitz D. A., Lobo P. I. Characterizaiton of two populations of human lymphocytes bearing easily detectable surface immunoglobulin. J Clin Invest. 1975 Dec;56(6):1464–1472. doi: 10.1172/JCI108227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Horwitz D. A., Niaudet P., Greaves M. F., Dorling J., Deteix P. Surface markers and electron microscopy of human blood L cells: a comparison with T and B lymphocytes. J Immunol. 1978 Aug;121(2):678–684. [PubMed] [Google Scholar]
  11. Kappler J. W., van der Hoven A., Dharmarajan U., Hoffmann M. Regulation of the immune response. IV. Antibody-mediated suppression of the immune response to haptens and heterologous erythrocyte antigens in vitro. J Immunol. 1973 Oct;111(4):1228–1235. [PubMed] [Google Scholar]
  12. Kölsch E., Oberbarnscheidt J., Brüner K., Heuer J. The Fc-receptor: its role in the transmission of differentiation signals. Immunol Rev. 1980;49:61–78. doi: 10.1111/j.1600-065x.1980.tb00426.x. [DOI] [PubMed] [Google Scholar]
  13. Lobo P. I. Characterization of a non-T, non-B human lymphocyte (L cell) with use of monoclonal antibodies. Its regulatory role in B lymphocyte function. J Clin Invest. 1981 Aug;68(2):431–438. doi: 10.1172/JCI110272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. NISONOFF A., MARKUS G., WISSLER F. C. Separation of univalent fragments of rabbit antibody by reduction of a single, labile disulphide bond. Nature. 1961 Jan 28;189:293–295. doi: 10.1038/189293a0. [DOI] [PubMed] [Google Scholar]
  15. Nicholas R., Sinclair S. C. Regulation of the immune response. I. Reduction in ability of specific antibody to inhibit long-lasting IgG immunological priming after removal of the Fc fragment. J Exp Med. 1969 Jun 1;129(6):1183–1201. doi: 10.1084/jem.129.6.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Oberbarnscheidt J., Kölsch E. Direct blockade of antigen-reactive B lymphocytes by immune complexes. An 'off' signal for precursors of IgM-producing cells provided by the linkage of antigen-and Fc-receptors. Immunology. 1978 Jul;35(1):151–157. [PMC free article] [PubMed] [Google Scholar]
  17. Ringdén O., Rynnel-Dagö B., Kunori T., Freijd A., Möller E. Antibody production and DNA synthesis of human lymphocyte subpopulations induced by PPD tuberculin. Clin Exp Immunol. 1979 Jun;36(3):528–535. [PMC free article] [PubMed] [Google Scholar]
  18. Stewart G. A., Smith A. K., Stanworth D. R. Biological activities associated with the Facb fragment of rabbit IgG. Immunochemistry. 1973 Nov;10(11):755–760. doi: 10.1016/0019-2791(73)90177-8. [DOI] [PubMed] [Google Scholar]

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