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. 1984 Aug;52(4):727–732.

Production of hybrids of mouse myeloma cells and protozoa which express parasite antigens.

M H Rodriguez-Lopez, P R Crocker, T J Harrison, G A Targett, D J Bradley
PMCID: PMC1454633  PMID: 6378772

Abstract

Mouse myeloma cells were fused with blood stage forms of the rodent malaria parasite Plasmodium chabaudi and with promastigotes of Leishmania donovani, the causative agent of kala-azar in man. The fusion was carried out by polyethylene glycol treatment. The parasites provided the enzyme which enabled the hybrids to grow in selective medium containing aminopterin. Clones of parasite-myeloma hybrids grown in continuous culture for up to 5 months expressed parasite antigen and induced anti-parasite antibodies in mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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