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. 1984 Aug;52(4):659–669.

Relation of intra-splenic migration of marginal zone B cells to antigen localization on follicular dendritic cells.

D Gray, D S Kumararatne, J Lortan, M Khan, I C MacLennan
PMCID: PMC1454654  PMID: 6378770

Abstract

Injection of heat-killed Escherichia coli into rats results in massive loss of IgM + ve, IgD - ve B cells from the marginal zones of their spleen within 4 hr. This is matched by a concomitant increase of cells with this phenotype in the splenic follicles. The marginal zone remains depleted and the follicles distended for about 16 hr, but the histological picture returns to normal within 24 hr. Surface marker analysis of blood and spleen B lymphocyte populations throughout the course of the migration suggest that there is intrasplenic migration of IgM + ve cells from marginal zone to follicles rather than via the circulation. Factors inhibiting localization of immune complex on follicular dendritic cells were assessed for their influence on marginal-zone B cell migration. Immune complex, injected 5 hr post-endotoxin administration localized poorly on follicular dendritic cells. While C3 depletion, by cobra venom, has no effect on marginal-zone B cell migration induced by endotoxin, it completely inhibits transport of heat-aggregated human gammaglobulin to follicular dendritic cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bazin H., Beckers A., Querinjean P. Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur J Immunol. 1974 Jan;4(1):44–48. doi: 10.1002/eji.1830040112. [DOI] [PubMed] [Google Scholar]
  2. Bazin H., Platteau B., Beckers A., Pauwels R. Differential effect of neonatal injections of anti-mu or anti-delta antibodies on the synthesis of IgM, IgD, IgE, IgA, IgG1, IgG2a, IgG2b, and IgG2c immunoglobulin classes. J Immunol. 1978 Nov;121(5):2083–2087. [PubMed] [Google Scholar]
  3. Brown J. C., Harris G., Papamichail M., Sljivić V. S., Holborow E. J. The localization of aggregated human -globulin in the spleens of normal mice. Immunology. 1973 Jun;24(6):955–968. [PMC free article] [PubMed] [Google Scholar]
  4. Cahill R. N., Frost H., Trnka Z. The effects of antigen on the migration of recirculating lymphocytes through single lymph nodes. J Exp Med. 1976 Apr 1;143(4):870–888. doi: 10.1084/jem.143.4.870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ford W. L., Simmonds S. J., Atkins R. C. Early cellular events in a systemic graft-vs.-host reaction. II. Autoradiographic estimates of the frequency of donor lymphocytes which respond to each Ag-B-determined antigenic complex. J Exp Med. 1975 Mar 1;141(3):681–696. doi: 10.1084/jem.141.3.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ford W. L. The recruitment of recirculating lymphocytes in the antigenically stimulated spleen. Specific and non-specific consequences of initiating a secondary antibody response. Clin Exp Immunol. 1972 Oct;12(2):243–254. [PMC free article] [PubMed] [Google Scholar]
  7. Gery I., Krüger J., Spiesel S. Z. Stimulation of B-lymphocytes by endotoxin. Reactions of thymus-deprived mice and karyotypic analysis of dividing cells in mice bearing T 6 T 6 thymus grafts. J Immunol. 1972 Apr;108(4):1088–1091. [PubMed] [Google Scholar]
  8. Gray D., MacLennan I. C., Bazin H., Khan M. Migrant mu+ delta+ and static mu+ delta- B lymphocyte subsets. Eur J Immunol. 1982 Jul;12(7):564–569. doi: 10.1002/eji.1830120707. [DOI] [PubMed] [Google Scholar]
  9. Gray D., McConnell I., Kumararatne D. S., MacLennan I. C., Humphrey J. H., Bazin H. Marginal zone B cells express CR1 and CR2 receptors. Eur J Immunol. 1984 Jan;14(1):47–52. doi: 10.1002/eji.1830140109. [DOI] [PubMed] [Google Scholar]
  10. Hall J. G., Morris B. The immediate effect of antigens on the cell output of a lymph node. Br J Exp Pathol. 1965 Aug;46(4):450–454. [PMC free article] [PubMed] [Google Scholar]
  11. Johnson G. D., Davidson R. S., McNamee K. C., Russell G., Goodwin D., Holborow E. J. Fading of immunofluorescence during microscopy: a study of the phenomenon and its remedy. J Immunol Methods. 1982 Dec 17;55(2):231–242. doi: 10.1016/0022-1759(82)90035-7. [DOI] [PubMed] [Google Scholar]
  12. Kearney J. F., Abney E. R. Immunoglobulin isotype expression. Contemp Top Immunobiol. 1978;8:245–265. doi: 10.1007/978-1-4684-0922-2_8. [DOI] [PubMed] [Google Scholar]
  13. Kumararatne D. S., Bazin H., MacLennan I. C. Marginal zones: the major B cell compartment of rat spleens. Eur J Immunol. 1981 Nov;11(11):858–864. doi: 10.1002/eji.1830111103. [DOI] [PubMed] [Google Scholar]
  14. Mitchell J., Abbot A. Antigens in immunity. XVI. A light and electron microscope study of antigen localization in the rat spleen. Immunology. 1971 Aug;21(2):207–224. [PMC free article] [PubMed] [Google Scholar]
  15. Morrison D. C., Ryan J. L. Bacterial endotoxins and host immune responses. Adv Immunol. 1979;28:293–450. doi: 10.1016/s0065-2776(08)60802-0. [DOI] [PubMed] [Google Scholar]
  16. Nieuwenhuis P., Ford W. L. Comparative migration of B- and T-Lymphocytes in the rat spleen and lymph nodes. Cell Immunol. 1976 May;23(2):254–267. doi: 10.1016/0008-8749(76)90191-x. [DOI] [PubMed] [Google Scholar]
  17. Nieuwenhuis P., Keuning F. J. Germinal centres and the origin of the B-cell system. II. Germinal centres in the rabbit spleen and popliteal lymph nodes. Immunology. 1974 Mar;26(3):509–519. [PMC free article] [PubMed] [Google Scholar]
  18. Papamichail M., Gutierrez C., Embling P., Johnson P., Holborow E. J., Pepys M. B. Complement dependence of localisation of aggregated IgG in germinal centres. Scand J Immunol. 1975;4(4):343–347. doi: 10.1111/j.1365-3083.1975.tb02635.x. [DOI] [PubMed] [Google Scholar]
  19. Peavy D. L., Adler W. H., Smith R. T. The mitogenic effects of endotoxin and staphylococcal enterotoxin B on mouse spleen cells and human peripheral lymphocytes. J Immunol. 1970 Dec;105(6):1453–1458. [PubMed] [Google Scholar]
  20. Pettersen J. C., Borgen D. F., Graupner K. C. A morphological and histochemical study of the primary and secondary immune responses in the rat spleen. Am J Anat. 1967 Sep;121(2):305–317. doi: 10.1002/aja.1001210209. [DOI] [PubMed] [Google Scholar]
  21. Pryjma J., Humphrey J. H. Prolonged C3 depletion by cobra venom factor in thymus-deprived mice and its implication for the role of C3 as an essential second signal for B-cell triggering. Immunology. 1975 Mar;28(3):569–576. [PMC free article] [PubMed] [Google Scholar]
  22. Smith J. B., Morris B. The response of the popliteal lymph node of the sheep to swine influenza virus. Aust J Exp Biol Med Sci. 1970 Feb;48(1):33–46. doi: 10.1038/icb.1970.4. [DOI] [PubMed] [Google Scholar]
  23. Sprent J., Lefkovits I. Effect of recent antigen priming on adoptive immune responses. IV. Antigen-induced selective recruitment of recirculating lymphocytes to the spleen demonstrable with a microculture system. J Exp Med. 1976 Jun 1;143(6):1289–1298. doi: 10.1084/jem.143.6.1289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Veerman A. J., Vries H. D. T- and B-areas in immune reactions. Volume changes in T and B cell compartments of the rat spleen following intravenous administration of a thymus-dependent (SRBC) and a thymus-independent (paratyphoid vaccin-endotoxin) antigen. A histometric study. Z Immunitatsforsch Exp Klin Immunol. 1976 Apr;151(3):202–218. [PubMed] [Google Scholar]
  25. Veerman A. J., van Ewijk W. White pulp compartments in the spleen of rats and mice. A light and electron microscopic study of lymphoid and non-lymphoid celltypes in T- and B-areas. Cell Tissue Res. 1975;156(4):417–441. doi: 10.1007/BF00225103. [DOI] [PubMed] [Google Scholar]
  26. Wallis V. J., Chaudhuri M. Two populations of murine LPS-responsive B lymphocytes differing in recirculatory capacity. Adv Exp Med Biol. 1982;149:147–152. doi: 10.1007/978-1-4684-9066-4_20. [DOI] [PubMed] [Google Scholar]
  27. Wood D. D., Cameron P. M., Poe M. T., Morris C. A. Resolution of a factor that enhances the antibody response of T cell-depleted murine splenocytes from several other monocyte products. Cell Immunol. 1976 Jan;21(1):88–96. doi: 10.1016/0008-8749(76)90330-0. [DOI] [PubMed] [Google Scholar]

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