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. 1988 Jan;63(1):63–69.

Anti-Trichomonas vaginalis monoclonal antibodies inducing complement-dependent cytotoxicity.

N Moav 1, E Draghi 1, A David 1, D Gold 1
PMCID: PMC1454709  PMID: 2448233

Abstract

Hybridomas producing monoclonal antibodies (mAbs) directed against Trichomonas vaginalis (Tv) have been produced in three fusions using mice immunized with live or killed Tv. The ELISA technique was used to test the binding activity of six out of the 48 mAbs produced. It was found that acetone fixation enhanced the binding activity of the antibodies and revealed hidden antigenic determinants. Thirty percent of the mAbs obtained from splenocytes of mice immunized with live Tv were of the IgG3 subtype. Two mAbs of the IgM and IgG3 subtypes demonstrated complement-fixing capacity. Incubation of these mAbs with live Tv and complement at 37 degrees for 30 min lysed the parasites. The lytic process was complement-dependent since in its absence the antibodies only agglutinated the parasites. The mAbs, when partially purified from ascitic fluids, had the same lytic activity as the native preparation. MAbs of the IgG1 subtype which did not fix complement, bound to Tv but did not lyse it. The lytic activity of the mAbs was not inhibited by cervico-vaginal secretions obtained from 14 women. It is suggested that mAbs could be used for diagnostic as well as for therapeutic purposes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alderete J. F. Antigen analysis of several pathogenic strains of Trichomonas vaginalis. Infect Immun. 1983 Mar;39(3):1041–1047. doi: 10.1128/iai.39.3.1041-1047.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Alderete J. F. Enzyme linked immunosorbent assay for detecting antibody to Trichomonas vaginalis: use of whole cells and aqueous extract as antigen. Br J Vener Dis. 1984 Jun;60(3):164–170. doi: 10.1136/sti.60.3.164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alderete J. F., Kasmala L., Metcalfe E., Garza G. E. Phenotypic variation and diversity among Trichomonas vaginalis isolates and correlation of phenotype with trichomonal virulence determinants. Infect Immun. 1986 Aug;53(2):285–293. doi: 10.1128/iai.53.2.285-293.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Alderete J. F., Kasmala L. Monoclonal antibody to a major glycoprotein immunogen mediates differential complement-independent lysis of Trichomonas vaginalis. Infect Immun. 1986 Sep;53(3):697–699. doi: 10.1128/iai.53.3.697-699.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Alderete J. F., Suprun-Brown L., Kasmala L., Smith J., Spence M. Heterogeneity of Trichomonas vaginalis and discrimination among trichomonal isolates and subpopulations with sera of patients and experimentally infected mice. Infect Immun. 1985 Sep;49(3):463–468. doi: 10.1128/iai.49.3.463-468.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brown M. T. Trichomoniasis. Practitioner. 1972 Nov;209(253):639–644. [PubMed] [Google Scholar]
  7. Catterall R. D. Trichomonal infections of the genital tract. Med Clin North Am. 1972 Sep;56(5):1203–1209. doi: 10.1016/s0025-7125(16)32345-8. [DOI] [PubMed] [Google Scholar]
  8. Cogne M., Brasseur P., Ballet J. J. Detection and characterization of serum antitrichomonal antibodies in urogenital trichomoniasis. J Clin Microbiol. 1985 Apr;21(4):588–592. doi: 10.1128/jcm.21.4.588-592.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Diamond L. S., Harlow D. R., Cunnick C. C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg. 1978;72(4):431–432. doi: 10.1016/0035-9203(78)90144-x. [DOI] [PubMed] [Google Scholar]
  10. Goldman P. Metronidazole: proven benefits and potential risks. Johns Hopkins Med J. 1980 Jul;147(1):1–9. [PubMed] [Google Scholar]
  11. KOTT H., ADLER S. A serological study of Trichomonas sp. parasitic in man. Trans R Soc Trop Med Hyg. 1961 Jul;55:333–344. doi: 10.1016/0035-9203(61)90102-x. [DOI] [PubMed] [Google Scholar]
  12. Kennett R. H., Denis K. A., Tung A. S., Klinman N. R. Hybrid plasmacytoma production: fusions with adult spleen cells, monoclonal spleen fragments, neonatal spleen cells and human spleen cells. Curr Top Microbiol Immunol. 1978;81:77–91. doi: 10.1007/978-3-642-67448-8_13. [DOI] [PubMed] [Google Scholar]
  13. Kuberski T. Evaluation of the indirect hemagglutination technique for study of Trichomonas vaginalis infections, particularly in men. Sex Transm Dis. 1978 Jul-Sep;5(3):97–102. doi: 10.1097/00007435-197807000-00004. [DOI] [PubMed] [Google Scholar]
  14. Kulda J., Vojtechovská M., Tachezy J., Demes P., Kunzová E. Metronidazole resistance of Trichomonas vaginalis as a cause of treatment failure in trichomoniasis--A case report. Br J Vener Dis. 1982 Dec;58(6):394–399. doi: 10.1136/sti.58.6.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  16. Mathews H. M., Healy G. R. Evaluation of two serological tests for Trichomonas vaginalis infection. J Clin Microbiol. 1983 May;17(5):840–843. doi: 10.1128/jcm.17.5.840-843.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ray D. K., Tendulkar J. S., Shrivastava V. B., Datta A. K., Nagarajan K. A metronidazole-resistant strain of Trichomonas vaginalis and its sensitivity to Go 10213. J Antimicrob Chemother. 1984 Oct;14(4):423–426. doi: 10.1093/jac/14.4.423. [DOI] [PubMed] [Google Scholar]
  18. Rossignol J. F., Maisonneuve H., Cho Y. W. Nitroimidazoles in the treatment of trichomoniasis, giardiasis, and amebiasis. Int J Clin Pharmacol Ther Toxicol. 1984 Feb;22(2):63–72. [PubMed] [Google Scholar]
  19. Taylor-Robinson D., Street D. A. Metronidazole-resistant trichomonas vaginalis. Lancet. 1981 Nov 14;2(8255):1107–1107. doi: 10.1016/s0140-6736(81)91300-3. [DOI] [PubMed] [Google Scholar]
  20. Torian B. E., Connelly R. J., Stephens R. S., Stibbs H. H. Specific and common antigens of Trichomonas vaginalis detected by monoclonal antibodies. Infect Immun. 1984 Jan;43(1):270–275. doi: 10.1128/iai.43.1.270-275.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Watt R. M., Philip A., Wos S. M., Sam G. J. Rapid assay for immunological detection of Trichomonas vaginalis. J Clin Microbiol. 1986 Oct;24(4):551–555. doi: 10.1128/jcm.24.4.551-555.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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