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. 1984 Sep;53(1):33–42.

The in vivo functions and properties of persisting cell-stimulating factor.

R M Crapper, I Clark-Lewis, J W Schrader
PMCID: PMC1454724  PMID: 6432685

Abstract

We present evidence that persisting (P) cell-stimulating factor (PSF), a T cell lymphokine, is produced and active in vivo. Mice injected in one footpad with keyhole limpet haemocyanin or intravenously with sheep erythrocytes had substantial increases in numbers of splenic P cell precursors; the increase following the sheep erythrocytes did not occur in athymic mice implying a dependence on T lymphocytes. The increase in P cell precursors correlated with the local release of PSF; thus cells from the ipsilateral draining lymph node of mice injected in one footpad with KLH, but not cells from the contralateral node, showed both increased numbers of P cell precursors and the production of PSF. PSF could, in other situations, enter the circulation and exert effects distal to its release. Mice bearing a localized tumour that produced PSF (WEHI-3B), but not those bearing a non-producing subline, showed both a significant increase in P cell precursors in the spleen and bone marrow, and a marked increase in the numbers of mast cells, megakaryocytes, metamyelocytes and polymorphs in the spleen. PSF was detected in the serum of the mice bearing the PSF-producing tumour. Following intravenous injection of PSF into normal mice there was a rapid initial clearance (t 1/2 4 min), followed after 10 mins by a phase of slower clearance (t 1/2 40 min). This was due to removal of PSF rather than inhibition or destruction by serum factors, as when PSF was mixed in vitro with mouse serum for 24 hr at 37 degrees, no activity was lost.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abbud Filho M., Dy M., Lebel B., Luffau G., Hamburger J. In vitro and in vivo histamine-producing cell-stimulating factor (or IL3) production during Nippostrongylus brasiliensis infection: coincidence with self-cure phenomenon. Eur J Immunol. 1983 Oct;13(10):841–845. doi: 10.1002/eji.1830131011. [DOI] [PubMed] [Google Scholar]
  2. Clark-Lewis I., Schrader J. W. P cell-stimulating factor: biochemical characterization of a new T cell-derived factor. J Immunol. 1981 Nov;127(5):1941–1947. [PubMed] [Google Scholar]
  3. Crapper R. M., Schrader J. W. Frequency of mast cell precursors in normal tissues determined by an in vitro assay: antigen induces parallel increases in the frequency of P cell precursors and mast cells. J Immunol. 1983 Aug;131(2):923–928. [PubMed] [Google Scholar]
  4. Dexter T. M., Garland J., Scott D., Scolnick E., Metcalf D. Growth of factor-dependent hemopoietic precursor cell lines. J Exp Med. 1980 Oct 1;152(4):1036–1047. doi: 10.1084/jem.152.4.1036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Donohue J. H., Rosenberg S. A. The fate of interleukin-2 after in vivo administration. J Immunol. 1983 May;130(5):2203–2208. [PubMed] [Google Scholar]
  6. Dy M., Lebel B., Kamoun P., Hamburger J. Histamine production during the anti-allograft response. Demonstration of a new lymphokine enhancing histamine synthesis. J Exp Med. 1981 Feb 1;153(2):293–309. doi: 10.1084/jem.153.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Garland J. M., Aldridge A., Wagstaffe J., Dexter T. M. Studies on the in vivo production of a lymphokine activity, interleukin 3 (IL-3) elaborated by lymphocytes and a myeloid leukaemic line in vitro and the fate of IL-3 dependent cell lines. Br J Cancer. 1983 Aug;48(2):247–259. doi: 10.1038/bjc.1983.180. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Good M. F., Boyd A. W., Nossal G. J. Analysis of true anti-hapten cytotoxic clones in limit dilution microcultures after correction for "anti-self" activity: precursor frequencies, Ly-2 and Thy-1 phenotype, specificity, and statistical methods. J Immunol. 1983 May;130(5):2046–2055. [PubMed] [Google Scholar]
  9. HALL J. G., MORRIS B. The lymph-borne cells of the immune response. Q J Exp Physiol Cogn Med Sci. 1963 Jul;48:235–247. doi: 10.1113/expphysiol.1963.sp001660. [DOI] [PubMed] [Google Scholar]
  10. Hall J. G., Morris B., Moreno G. D., Bessis M. C. The ultrastructure and function of the cells in lymph following antigenic stimulation. J Exp Med. 1967 Jan 1;125(1):91–110. doi: 10.1084/jem.125.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ihle J. N., Keller J., Henderson L., Klein F., Palaszynski E. Procedures for the purification of interleukin 3 to homogeneity. J Immunol. 1982 Dec;129(6):2431–2436. [PubMed] [Google Scholar]
  12. Ihle J. N., Keller J., Oroszlan S., Henderson L. E., Copeland T. D., Fitch F., Prystowsky M. B., Goldwasser E., Schrader J. W., Palaszynski E. Biologic properties of homogeneous interleukin 3. I. Demonstration of WEHI-3 growth factor activity, mast cell growth factor activity, p cell-stimulating factor activity, colony-stimulating factor activity, and histamine-producing cell-stimulating factor activity. J Immunol. 1983 Jul;131(1):282–287. [PubMed] [Google Scholar]
  13. Mayrhofer G., Fisher R. Mast cells in severely T-cell depleted rats and the response to infestation with Nippostrongylus brasiliensis. Immunology. 1979 May;37(1):145–155. [PMC free article] [PubMed] [Google Scholar]
  14. Metcalf D. Sources and biology of regulatory factors active on mouse myeloid leukemic cells. J Cell Physiol Suppl. 1982;1:175–183. doi: 10.1002/jcp.1041130425. [DOI] [PubMed] [Google Scholar]
  15. Mühlradt P. F., Opitz H. G. Clearance of interleukin 2 from the blood of normal and T cell-depleted mice. Eur J Immunol. 1982 Nov;12(11):983–985. doi: 10.1002/eji.1830121117. [DOI] [PubMed] [Google Scholar]
  16. Parmentier H. K., Ruitenberg E. J., Elgersma A. Thymus dependence of the adoptive transfer of intestinal mastocytopoiesis in Trichinella spiralis-infected mice. Int Arch Allergy Appl Immunol. 1982;68(3):260–267. doi: 10.1159/000233109. [DOI] [PubMed] [Google Scholar]
  17. Schrader J. W. Bone marrow differentiation in vitro. Crit Rev Immunol. 1983;4(3):197–277. [PubMed] [Google Scholar]
  18. Schrader J. W., Clark-Lewis I. A T cell-derived factor stimulating multipotential hemopoietic stem cells: molecular weight and distinction from T cell growth factor and T cell-derived granulocyte-macrophage colony-stimulating factor. J Immunol. 1982 Jul;129(1):30–35. [PubMed] [Google Scholar]
  19. Schrader J. W. In in vitro production and cloning of the P cell, a bone marrow-derived null cell that expresses H-2 and Ia-antigens, has mast cell-like granules, and is regulated by a factor released by activated T cells. J Immunol. 1981 Feb;126(2):452–458. [PubMed] [Google Scholar]
  20. Shadduck R. K., Waheed A., Porcellini A., Rizzoli V., Pigoli G. Physiologic distribution of colony-stimulating factor in vivo. Blood. 1979 Oct;54(4):894–905. [PubMed] [Google Scholar]
  21. Yung Y. P., Eger R., Tertian G., Moore M. A. Long-term in vitro culture of murine mast cells. II. Purification of a mast cell growth factor and its dissociation from TCGF. J Immunol. 1981 Aug;127(2):794–799. [PubMed] [Google Scholar]

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