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. 1988 Apr;63(4):569–574.

Identification of isotypes and allotypes of bovine immunoglobulin M with monoclonal antibodies.

J Naessens 1, J Newson 1, D J Williams 1, V Lutje 1
PMCID: PMC1454783  PMID: 3259201

Abstract

Epitopes specific for IgM on peripheral blood lymphocytes from 47 cattle were examined with three class-specific monoclonal antibodies, IL-A30, IL-A50 and B5/4. In all 47 animals tested, mAb IL-A30 detected a similar percentage of peripheral blood lymphocytes as a mAb that recognizes all immunoglobulin classes. However, in some animals mAbs IL-A50 and B5/4 detected a lower percentage of B cells compared with IL-A30 or the mAb against total Ig. They both reacted with a proportion of the serum IgM from these animals, while IL-A30 reacted with all serum IgM. Therefore, it is probable that mAb-A30 recognizes an IgM isotypic determinant and mAbs IL-A50 and B5/4 recognize different IgM allotypic determinants. Using mAb IL-A30 it was found that the percentage of peripheral blood IgM+ lymphocytes varied widely between healthy cattle (4-31%) but remained constant, with only minor variations, within individual animals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Gilman-Sachs A., Roux K. H., Horng W. J., Dray S. Characterization of additional rabbit IgM allotypes and the effect of suppression of a VH locus allotypes on the expression of n C mu locus allotypes. J Immunol. 1982 Jan;128(1):451–456. [PubMed] [Google Scholar]
  2. Kipps T. J., Vaughan J. H. Genetic influence on the levels of circulating CD5 B lymphocytes. J Immunol. 1987 Aug 15;139(4):1060–1064. [PubMed] [Google Scholar]
  3. LEVY H. B., SOBER H. A. A simple chromatographic method for preparation of gamma globulin. Proc Soc Exp Biol Med. 1960 Jan;103:250–252. doi: 10.3181/00379727-103-25476. [DOI] [PubMed] [Google Scholar]
  4. Naessens J., Newson J., Bensaid A., Teale A. J., Magondu J. G., Black S. J. De novo expression of T cell markers on Theileria parva-transformed lymphoblasts in cattle. J Immunol. 1985 Dec;135(6):4183–4188. [PubMed] [Google Scholar]
  5. Okumura K., Julius M. H., Tsu T., Herzenberg L. A., Herzenberg L. A. Demonstration that IgG memory is carried by IgG-bearing cells. Eur J Immunol. 1976 Jul;6(7):467–472. doi: 10.1002/eji.1830060704. [DOI] [PubMed] [Google Scholar]
  6. Pinder M., Musoke A. J., Morrison W. I., Roelants G. E. The bovine lymphoid system. III. A monoclonal antibody specific for bovine cell surface and serum IgM. Immunology. 1980 Jul;40(3):359–365. [PMC free article] [PubMed] [Google Scholar]
  7. Roland J., Cazenave P. A. Un motif allotypique probablement de nature glucidique porté par le fragment Fab des IgG de lapin. Ann Immunol (Paris) 1976 Sep-Oct;127(5):633–655. [PubMed] [Google Scholar]
  8. Senogles D. R., Muscoplat C. C., Paul P. S., Johnson D. W. Ontogeny of circulating B lymphocytes in neonatal calves. Res Vet Sci. 1978 Jul;25(1):34–36. [PubMed] [Google Scholar]
  9. van Zaane D., Ijzerman J. Monoclonal antibodies against bovine immunoglobulins and their use in isotype-specific ELISAs for rotavirus antibody. J Immunol Methods. 1984 Sep 4;72(2):427–441. doi: 10.1016/0022-1759(84)90011-5. [DOI] [PMC free article] [PubMed] [Google Scholar]

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