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. 1988 Apr;63(4):721–727.

Immunolocalization of thymosin alpha 1, thymopoietin and thymulin in mouse thymic epithelial cells at different stages of culture: a light and electron microscopic study.

N Fabien 1, C Auger 1, J C Monier 1
PMCID: PMC1454785  PMID: 3284819

Abstract

The secretory evolution of the thymic hormones (thymulin, thymosin alpha 1 and thymopoietin) in cultured thymic reticuloepithelial cells (TREC) was studied by immunocytochemical techniques using monoclonal anti-thymulin or anti-thymosin alpha 1 and polyclonal anti-thymopoietin antibodies (Ab). The culture of TREC was performed with a medium where L-valine was replaced by D-valine, thus ensuring rapid and selective development of these cells. The number of thymulin, thymosin alpha 1 or thymopoietin-containing cells increased progressively from Day 6 to Day 12 of the culture. The localization of the three thymic hormones within the TREC also varied according to the age of the culture. By light microscopy the staining of the three hormones was localized in some cytoplasmic granules at the beginning of the culture and at Day 90, while at Day 12 it was throughout the cytoplasm. In electron microscopy these localizations corresponded respectively to vacuoles of different sizes and to cytosol. All these results show that the synthesis and excretion of thymulin, thymosin alpha 1 and thymopoietin evolve during the development of TREC in culture.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auger C., Monier J. C., Dardenne M., Pleau J. M., Bach J. F. Identification of FTS (facteur thymique serique) on thymus ultrathin sections using monoclonal antibodies. Immunol Lett. 1982 Oct;5(4):213–216. doi: 10.1016/0165-2478(82)90137-7. [DOI] [PubMed] [Google Scholar]
  2. Auger C., Monier J. C., Savino W., Dardenne M. Localization of thymulin (FTS-Zn) in mouse thymus. Comparative data using monoclonal antibodies following different plastic embedding procedures. Biol Cell. 1984;52(2):139–146. doi: 10.1111/j.1768-322x.1985.tb00331.x. [DOI] [PubMed] [Google Scholar]
  3. Auger C., Stahli C., Fabien N., Monier J. C. Intracellular localization of thymosin alpha 1 by immunoelectron microscopy using a monoclonal antibody. J Histochem Cytochem. 1987 Feb;35(2):181–187. doi: 10.1177/35.2.3540103. [DOI] [PubMed] [Google Scholar]
  4. Cohen S., Berrih S., Dardenne M., Bach J. F. Feedback regulation of the secretion of a thymic hormone (thymulin) by human thymic epithelial cells in culture. Thymus. 1986;8(3):109–119. [PubMed] [Google Scholar]
  5. Goldstein A. L., Low T. L., McAdoo M., McClure J., Thurman G. B., Rossio J., Lai C. Y., Chang D., Wang S. S., Harvey C. Thymosin alpha1: isolation and sequence analysis of an immunologically active thymic polypeptide. Proc Natl Acad Sci U S A. 1977 Feb;74(2):725–729. doi: 10.1073/pnas.74.2.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldstein G., Scheid M. P., Boyse E. A., Schlesinger D. H., Van Wauwe J. A synthetic pentapeptide with biological activity characteristic of the thymic hormone thymopoietin. Science. 1979 Jun 22;204(4399):1309–1310. doi: 10.1126/science.451537. [DOI] [PubMed] [Google Scholar]
  7. Goldstein G. The isolation of thymopoietin (thymin). Ann N Y Acad Sci. 1975 Feb 28;249:177–185. doi: 10.1111/j.1749-6632.1975.tb29067.x. [DOI] [PubMed] [Google Scholar]
  8. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  9. Haritos A. A., Blacher R., Stein S., Caldarella J., Horecker B. L. Primary structure of rat thymus prothymosin alpha. Proc Natl Acad Sci U S A. 1985 Jan;82(2):343–346. doi: 10.1073/pnas.82.2.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jambon B., Montagne P., Bene M. C., Brayer M. P., Faure G., Duheille J. Immunohistologic localization of "facteur thymique serique" (FTS) in human thymic epithelium. J Immunol. 1981 Nov;127(5):2055–2059. [PubMed] [Google Scholar]
  11. Monier J. C., Dardenne M., Pléau J. M., Schmitt D., Deschaux P., Bach J. F. Characterization of facteur thymique sérique (FTS) in the thymus. I. Fixation of anti-FTS antibodies on thymic reticulo-epithelial cells. Clin Exp Immunol. 1980 Dec;42(3):470–476. [PMC free article] [PubMed] [Google Scholar]
  12. Nieburgs A. C., Picciano P. T., Korn J. H., McCalister T., Allred C., Cohen S. In vitro growth and maintenance of two morphologically distinct populations of thymic epithelial cells. Cell Immunol. 1985 Feb;90(2):439–450. doi: 10.1016/0008-8749(85)90208-4. [DOI] [PubMed] [Google Scholar]
  13. Rothenberg E., Lugo J. P. Differentiation and cell division in the mammalian thymus. Dev Biol. 1985 Nov;112(1):1–17. doi: 10.1016/0012-1606(85)90114-9. [DOI] [PubMed] [Google Scholar]
  14. Savino W., Dardenne M., Papiernik M., Bach J. F. Thymic hormone-containing cells. Characterization and localization of serum thymic factor in young mouse thymus studied by monoclonal antibodies. J Exp Med. 1982 Aug 1;156(2):628–633. doi: 10.1084/jem.156.2.628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Savino W., Dardenne M. Thymic hormone-containing cells VI. Immunohistologic evidence for the simultaneous presence of thymulin, thymopoietin and thymosin alpha 1 in normal and pathological human thymuses. Eur J Immunol. 1984 Nov;14(11):987–991. doi: 10.1002/eji.1830141105. [DOI] [PubMed] [Google Scholar]
  16. Schmitt D., Monier J. C., Dardenne M., Pleau J. M., Bach J. F. Location of FTS (facteur thymique sérique) in the thymus of normal and auto-immune mice. Thymus. 1982 Jul;4(4):221–231. [PubMed] [Google Scholar]
  17. Schmitt D., Monier J. C., Dardenne M., Pleau J. M., Deschaux P., Bach J. F. Cytoplasmic localization of FTS (facteur thymique sérique) in thymic epithelial cells. An immunoelectronmicroscopical study. Thymus. 1980 Dec;2(3):177–186. [PubMed] [Google Scholar]
  18. Small M., Barr-Nea L., Aronson M. Culture of thymic epithelial cells from mice and age-related studies on the growing cells. Eur J Immunol. 1984 Oct;14(10):936–942. doi: 10.1002/eji.1830141014. [DOI] [PubMed] [Google Scholar]
  19. Tougard C., Picart R. Use of pre-embedding ultrastructural immunocytochemistry in the localization of a secretory product and membrane proteins in cultured prolactin cells. Am J Anat. 1986 Feb-Mar;175(2-3):161–177. doi: 10.1002/aja.1001750206. [DOI] [PubMed] [Google Scholar]
  20. Viamontes G. I., Audhya T., Goldstein G. Immunohistochemical localization of thymopoietin with an antiserum to synthetic Cys-thymopoietin28-39. Cell Immunol. 1986 Jul;100(2):305–313. doi: 10.1016/0008-8749(86)90031-6. [DOI] [PubMed] [Google Scholar]

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