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. 1988 Apr;63(4):729–731.

Lymphokine-activated killer cells lyse human renal cancer cell lines and cultured normal kidney cells.

A M Miltenburg 1, M E Meijer-Paape 1, M R Daha 1, L C Paul 1
PMCID: PMC1454787  PMID: 3259208

Abstract

In this study, we investigated whether or not lymphokine-activated killer (LAK) cells can damage renal tissue and therefore whether they may contribute to graft destruction during kidney allograft rejection. Human peripheral blood mononuclear cells were activated with a lymphokine preparation and the resulting LAK cells were tested against kidney cells from various sources. Renal cancer cells as well as cultured normal kidney cells were efficiently lysed by LAK cells, as assessed with Cr-labelled target cells, showing that both cell types are sensitive to LAK cell-mediated cytolysis.

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Selected References

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  1. Bishop G. A., Suranyi M. J., Hall B. M., Duggin G. G., Tiller D. J., Horvath J. S. Induction of HLA-DR antigens on cultured human kidney cells. Transplant Proc. 1986 Apr;18(2):290–292. [PubMed] [Google Scholar]
  2. Damle N. K., Doyle L. V., Bender J. R., Bradley E. C. Interleukin 2-activated human lymphocytes exhibit enhanced adhesion to normal vascular endothelial cells and cause their lysis. J Immunol. 1987 Mar 15;138(6):1779–1785. [PubMed] [Google Scholar]
  3. Hanto D. W., Hopt U. T., Hoffman R., Simmons R. L. Recruitment of unsensitized circulating lymphocytes to sites of allogeneic cellular interactions. Transplantation. 1982 May;33(5):541–546. doi: 10.1097/00007890-198205000-00015. [DOI] [PubMed] [Google Scholar]
  4. Henny F. C., Weening J. J., Baldwin W. M., Oljans P. J., Tanke H. J., van Es L. A., Paul L. C. Expression of HLA-DR antigens on peripheral blood T lymphocytes and renal graft tubular epithelial cells in association with rejection. Transplantation. 1986 Nov;42(5):479–483. doi: 10.1097/00007890-198611000-00007. [DOI] [PubMed] [Google Scholar]
  5. Miltenburg A. M., Meijer-Paape M. E., Daha M. R., Paul L. C. Endothelial cell lysis induced by lymphokine-activated human peripheral blood mononuclear cells. Eur J Immunol. 1987 Sep;17(9):1383–1386. doi: 10.1002/eji.1830170926. [DOI] [PubMed] [Google Scholar]
  6. Moreau J. F., Bonneville M., Peyrat M. A., Godard A., Jacques Y., Desgranges C., Soulillou J. P. T lymphocyte cloning from rejected human kidney allografts. Growth frequency and functional/phenotypic analysis. J Clin Invest. 1986 Oct;78(4):874–879. doi: 10.1172/JCI112674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ochoa A. C., Gromo G., Alter B. J., Sondel P. M., Bach F. H. Long-term growth of lymphokine-activated killer (LAK) cells: role of anti-CD3, beta-IL 1, interferon-gamma and -beta. J Immunol. 1987 Apr 15;138(8):2728–2733. [PubMed] [Google Scholar]
  8. Rosenberg S. A., Lotze M. T. Cancer immunotherapy using interleukin-2 and interleukin-2-activated lymphocytes. Annu Rev Immunol. 1986;4:681–709. doi: 10.1146/annurev.iy.04.040186.003341. [DOI] [PubMed] [Google Scholar]
  9. Ueda R., Shiku H., Pfreundschuh M., Takahashi T., Li L. T., Whitmore W. F., Oettgen H. F., Old L. J. Cell surface antigens of human renal cancer defined by autologous typing. J Exp Med. 1979 Sep 19;150(3):564–579. doi: 10.1084/jem.150.3.564. [DOI] [PMC free article] [PubMed] [Google Scholar]

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