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. 1988 Apr;63(4):563–567.

Studies of a human lambda-chain epitope related to a complementarity-determining region.

H S Kim 1, H F Deutsch 1
PMCID: PMC1454789  PMID: 2452787

Abstract

A tryptic non-adecapeptide representing the 24-42 sequence of the MCG lambda-type Bence-Jones protein, and which contains its entire complementarity-determining region-1, was isolated. The peptide was utilized in preparing an affinity column that was used to isolate an antibody having the reactivity of a previously employed idiotypic antibody to MCG. This antibody preparation, as well as 13 monoclonal mouse antibodies to human lambda-chains, was employed in an enzyme-linked immunoassay to detect other Bence-Jones proteins with this serologic specificity. The results obtained with two of the monoclonal antibodies suggest that the epitope in question is a noncontiguous one.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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