Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1984 Oct;53(2):207–214.

In vitro synthesis of IgE by human lymphocytes. III. IgE-potentiating activity of culture supernatants from Epstein-Barr virus (EBV) transformed B cells.

M Sarfati, E Rector, M Rubio-Trujillo, K Wong, A H Sehon, G Delespesse
PMCID: PMC1454817  PMID: 6092268

Abstract

Seven Epstein--Barr virus (EBV)-transformed B cell lines were derived from circulating lymphocytes of two atopic and two non-atopic individuals, two preparations of cord blood lymphocytes and one tonsillar lymphocyte preparation. All the cell lines contained a significant proportion of cells expressing Fc epsilon R as detected by rosette formation with IgE-coated bovine erythrocytes (E-IgE) and by flow cytometry using IgE-linked to fluorescent microspheres. None of the cell lines displayed FcR for IgA, IgM or IgG. The cell-free supernatants (CFS) of EBV-transformed cells contained IgE-binding factors (IgE-BFs) detected by their ability to inhibit the binding to RPMI 8866 cells of either E-IgE or IgE-linked to microspheres. Whereas these CFS enhanced the synthesis of IgE and suppressed the synthesis of IgG by purified B lymphocytes isolated from the blood of allergic donors and cultured in the absence of stimulant, their effect on the synthesis of IgA or IgM was not predictable. CFS significantly enhanced the secretion of IgE by the U266 myeloma cell line without interfering with secretion of IgM, IgG or IgA by EBV-transformed cells. These data are in accord with similar properties of RPMI 8866 cells and suggest that B lymphocytes might play a regulating role in the IgE synthesis.

Full text

PDF
207

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beneke J. S., Qualtiere L. F., Nesheim M. C., Pearson G. R. Purification and biochemical characterization of an inhibitor of DNA synthesis produced by an Epstein-Barr virus-transformed B cell line. J Immunol. 1980 Jun;124(6):2950–2955. [PubMed] [Google Scholar]
  2. Böyum A. Separation of leukocytes from blood and bone marrow. Introduction. Scand J Clin Lab Invest Suppl. 1968;97:7–7. [PubMed] [Google Scholar]
  3. Deguchi H., Suemura M., Ishizaka A., Ozaki Y., Kishimoto S., Yamamura Y., Kishimoto T. IgE class-specific suppressor T cells and factors in humans. J Immunol. 1983 Dec;131(6):2751–2756. [PubMed] [Google Scholar]
  4. Finegold I., Fahey J. L., Granger H. Synthesis of immunoglobulins by human cell lines in tissue culture. J Immunol. 1967 Nov;99(5):839–848. [PubMed] [Google Scholar]
  5. Gonzalez-Molina A., Spiegelberg H. L. A subpopulation of normal human peripheral B lymphcytes that bind IgE. J Clin Invest. 1977 Apr;59(4):616–624. doi: 10.1172/JCI108679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gonzalez-Molina A., Spiegelberg H. L. Binding of IgE myeloma proteins to human cultured lymphoblastoid cells. J Immunol. 1976 Nov;117(5 PT2):1838–1845. [PubMed] [Google Scholar]
  7. Granger G. A., Moore G. E., White J. G., Matzinger P., Sundsmo J. S., Shupe S., Kolb W. P., Kramer J., Glade P. R. Production of lymphotoxin and migration inhibitory factor by established human lymphocytic cell lines. J Immunol. 1970 Jun;104(6):1476–1485. [PubMed] [Google Scholar]
  8. Hassner A., Saxon A. Inhibition of ongoing myeloma IgE synthesis in vitro by activated human T cells. J Immunol. 1983 Apr;130(4):1567–1572. [PubMed] [Google Scholar]
  9. Hellström U., Spiegelberg H. L. Characterization of human lymphocytes bearing Fc receptors for IgE isolated from blood and lymphoid organs. Scand J Immunol. 1979;9(1):75–86. doi: 10.1111/j.1365-3083.1979.tb02709.x. [DOI] [PubMed] [Google Scholar]
  10. Ishizaka K., Sandberg K. Formation of IgE binding factors by human T lymphocytes. J Immunol. 1981 May;126(5):1692–1696. [PubMed] [Google Scholar]
  11. Kaplan M. E., Clark C. An improved rosetting assay for detection of human T lymphocytes. J Immunol Methods. 1974 Jul;5(2):131–135. doi: 10.1016/0022-1759(74)90003-9. [DOI] [PubMed] [Google Scholar]
  12. Kishimoto T. IgE class-specific suppressor T cells and regulation of the IgE response. Prog Allergy. 1982;32:265–317. [PubMed] [Google Scholar]
  13. Löwy I., Brezin C., Neauport-Sautes C., Theze J., Fridman W. H. Isotype regulation of antibody production: T-cell hybrids can be selectively induced to produce IgG1 and IgG2 subclass-specific suppressive immunoglobulin-binding factors. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2323–2327. doi: 10.1073/pnas.80.8.2323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Moretta L., Mingari M. C., Moretta A., Cooper M. D. Human T lymphocyte subpopulations: studies of the mechanism by which T cells bearing Fc receptors for IgG suppress T-dependent B cell differentiation induced by pokeweed mitogen. J Immunol. 1979 Mar;122(3):984–990. [PubMed] [Google Scholar]
  15. Mosier D. E., Coppleson L. W. A THREE-CELL INTERACTION REQUIRED FOR THE INDUCTION OF THE PRIMARY IMMUNE RESPONSE in vitro. Proc Natl Acad Sci U S A. 1968 Oct;61(2):542–547. doi: 10.1073/pnas.61.2.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Revillard J. P., Lê Thi Bich-Thuy L. T. Régulation de la production d'immunoglobulines: rôle des récepteurs cellulaires de la partie Fc de ces molécules. Ann Immunol (Paris) 1982 Sep-Oct;133D(2):199–210. [PubMed] [Google Scholar]
  17. Romagnani S., Maggi E., Del Prete G. F., Ricci M. IgE synthesis in vitro induced by T cell factors from patients with elevated serum IgE levels. Clin Exp Immunol. 1983 Apr;52(1):85–88. [PMC free article] [PubMed] [Google Scholar]
  18. Sarfati M., Rector E., Wong K., Rubio-Trujillo M., Sehon A. H., Delespesse G. In vitro synthesis of IgE by human lymphocytes. II. Enhancement of the spontaneous IgE synthesis by IgE-binding factors secreted by RPMI 8866 lymphoblastoid B cells. Immunology. 1984 Oct;53(2):197–205. [PMC free article] [PubMed] [Google Scholar]
  19. Sarfati M., Rubio-Trujillo M., Wong K., Rector E., Sehon A. H., Delespesse G. In vitro synthesis of IgE by human lymphocytes. I. The spontaneous secretion of IgE by B lymphocytes from allergic individuals: a model to investigate the regulation of human IgE synthesis. Immunology. 1984 Oct;53(2):187–196. [PMC free article] [PubMed] [Google Scholar]
  20. Saryan J. A., Leung D. Y., Geha R. S. Induction of human IgE synthesis by a factor derived from T cells of patients with hyper-IgE states. J Immunol. 1983 Jan;130(1):242–247. [PubMed] [Google Scholar]
  21. Spiegelberg H. L. Lymphocytes bearing Fc receptors for IgE. Immunol Rev. 1981;56:199–218. doi: 10.1111/j.1600-065x.1981.tb01052.x. [DOI] [PubMed] [Google Scholar]
  22. Spiegelberg H. L., O'Connor R. D., Simon R. A., Mathison D. A. Lymphocytes with immunoglobulin E Fc receptors in patients with atopic disorders. J Clin Invest. 1979 Sep;64(3):714–720. doi: 10.1172/JCI109514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Suemura M., Ishizaka A., Kobatake S., Sugimura K., Maeda K., Nakanishi K., Kishimoto S., Yamamura Y., Kishimoto T. Inhibition of IgE production in B hybridomas by IgE class-specific suppressor factor from T hybridomas. J Immunol. 1983 Mar;130(3):1056–1060. [PubMed] [Google Scholar]
  24. Yodoi J., Adachi M., Teshigawara K., Miyamainaba M., Masuda T., Fridman W. H. T cell hybridomas coexpressing Fc receptors (FcR) for different isotypes. II. IgA-induced formation of suppressive IgA binding factor(s) by a murine T hybridoma bearing Fc gamma R and Fc alpha R. J Immunol. 1983 Jul;131(1):303–310. [PubMed] [Google Scholar]
  25. Yodoi J., Hirashima M., Ishizaka K. Regulatory role of IgE-binding factors from rat T lymphocytes. V. The carbohydrate moieties in IgE-potentiating factors and IgE-suppressive factors. J Immunol. 1982 Jan;128(1):289–295. [PubMed] [Google Scholar]
  26. Yodoi J., Ishizaka K. Induction of Fc epsilon-receptor bearing cells in vitro in human peripheral lymphocytes. J Immunol. 1980 Feb;124(2):934–938. [PubMed] [Google Scholar]
  27. Yoshida T., Kuratsuji T., Takada A., Takada Y., Minowada J., Cohen S. Lymphokine-like factors produced by human lymphoid cell lines with B or T cell surface markers. J Immunol. 1976 Aug;117(2):548–554. [PubMed] [Google Scholar]
  28. Zajac B. A., Henle W., Henle G. Autogenous and virus-induced interferons from lines of lymphoblastoid cells. Cancer Res. 1969 Aug;29(8):1467–1475. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES