Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1985 Jan;54(1):89–96.

B-cell function in common variable immunodeficiency: suppression of in vitro anti-sheep erythrocytes antibody production by T cells and monocytes.

S Pollack, Y Reisner, B Koziner, R A Good, M K Hoffman
PMCID: PMC1454853  PMID: 3156090

Abstract

Peripheral blood mononuclear (PBM) cells from five of 21 patients with common variable immunodeficiency (CVI) stimulated in vitro by antigen produced antibodies normally. In 11 of the 16 non-responders, removal of adherent suppressor cells by Sephadex G-10 or removal of monocytes or OKT8 positive suppressor lymphocytes by other means of separation made antibody responses demonstrable. Cells adherent to the G-10 column had suppressor function in the autologous antigen driven system; the suppressor activity appeared to be due to adherent T cells in some patients and to adherent monocytes in others. The suppressor cell functions in these patients did not correlate significantly with the number of cells positive for monoclonal antibodies OKT8 or Leu-2. Factors suppressing antibody production in this system were found in normal serum but usually not in serum of the patients with CVI studied. Substances in normal serum which enhance antibody production in this system when added late in culture were present in serum of patients with CVI. Any abnormality of interleukin-1 production by monocytes of these patients did not seem relevant to the immunodeficiency.

Full text

PDF
89

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Galanaud P. In vitro antibody response to trinitrophenyl-polyacrylamide beads. Immunol Rev. 1979;45:141–161. doi: 10.1111/j.1600-065x.1979.tb00276.x. [DOI] [PubMed] [Google Scholar]
  2. Haber P. L., Kubagawa H., Cooper M. D. Epstein-Barr virus-induced immunoglobulin synthesis by B cells from individuals with late-onset panhypogammaglobulinemia. J Clin Immunol. 1983 Jul;3(3):253–259. doi: 10.1007/BF00915349. [DOI] [PubMed] [Google Scholar]
  3. Herrod H. G., Buckley R. H. Use of a human plaque-forming cell assay to study peripheral blood bursa-equivalent cell activation and excessive suppressor cell activity in humoral immunodeficiency. J Clin Invest. 1979 May;63(5):868–876. doi: 10.1172/JCI109386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hoffmann M. K. Antigen-specific induction and regulation of antibody synthesis in cultures of human peripheral blood mononuclear cells. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1139–1143. doi: 10.1073/pnas.77.2.1139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hoffmann M. K., Pollack S., Krown S. E., Mittler R. S. Deletion of active human suppressor T lymphocytes from peripheral blood by Sephadex G-10 filtration. J Immunol Methods. 1982 Dec 30;55(3):327–336. doi: 10.1016/0022-1759(82)90092-8. [DOI] [PubMed] [Google Scholar]
  6. Kotzin B. L., Benike C. J., Engleman E. G. Induction of immunoglobulin-secreting cells in the allogeneic mixed leukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol. 1981 Sep;127(3):931–935. [PubMed] [Google Scholar]
  7. Koziner B., McKenzie S., Straus D., Clarkson B., Good R. A., Siegal F. P. Cell marker analysis in acute monocytic leukemias. Blood. 1977 Jun;49(6):895–901. [PubMed] [Google Scholar]
  8. Ledbetter J. A., Evans R. L., Lipinski M., Cunningham-Rundles C., Good R. A., Herzenberg L. A. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981 Feb 1;153(2):310–323. doi: 10.1084/jem.153.2.310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mitsuya H., Osaki K., Tomino S., Katsuki T., Kishimoto S. Pathophysiologic analysis of peripheral blood lymphocytes from patients with primary immunodeficiency. I. Ig synthesis by peripheral blood lymphocytes stimulated with either pokeweed mitogen or Epstein-Barr virus in vitro. J Immunol. 1981 Jul;127(1):311–315. [PubMed] [Google Scholar]
  11. Pahwa S. G., Good R. A., Hoffmann M. K. The role of mitogens and antigens in the generation of antibody-producing human B lymphocytes. J Clin Immunol. 1981 Apr;1(2):101–105. doi: 10.1007/BF00915386. [DOI] [PubMed] [Google Scholar]
  12. Pahwa S. G., Hoffman M. K., Pahwa R. N., Good R. A. Polyclonal and antigen-specific B-cell responses in patients with common variable immunodeficiency. J Clin Immunol. 1982 Jul;2(3):205–213. doi: 10.1007/BF00915223. [DOI] [PubMed] [Google Scholar]
  13. Pereira S., Webster D., Platts-Mills T. Immature B cells in fetal development and immunodeficiency: studies of IgM, IgG, IgA and IgD production in vitro using Epstein-Barr virus activation. Eur J Immunol. 1982 Jul;12(7):540–546. doi: 10.1002/eji.1830120703. [DOI] [PubMed] [Google Scholar]
  14. Pollack S., Micali A., Kinne D. W., Enker W. E., Geller N., Oettgen H. F., Hoffmann M. K. Endotoxin-induced in vitro release of interleukin-1 by cancer patients' monocytes: relation to stage of disease. Int J Cancer. 1983 Dec 15;32(6):733–736. doi: 10.1002/ijc.2910320613. [DOI] [PubMed] [Google Scholar]
  15. Reinherz E. L., Kung P. C., Goldstein G., Schlossman S. F. A monoclonal antibody reactive with the human cytotoxic/suppressor T cell subset previously defined by a heteroantiserum termed TH2. J Immunol. 1980 Mar;124(3):1301–1307. [PubMed] [Google Scholar]
  16. Reinherz E. L., Rubinstein A., Geha R. S., Strelkauskas A. J., Rosen F. S., Schlossman S. F. Abnormalities of immunoregulatory T cells in disorders of immune function. N Engl J Med. 1979 Nov 8;301(19):1018–1022. doi: 10.1056/NEJM197911083011902. [DOI] [PubMed] [Google Scholar]
  17. Reisner Y., Pahwa S., Chiao J. W., Sharon N., Evans R. L., Good R. A. Separation of antibody helper and antibody suppressor human T cells by using soybean agglutinin. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6778–6782. doi: 10.1073/pnas.77.11.6778. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rubinstein A., Sicklick M., Mehra V., Rosen F. S., Levey R. H. Antihelper T cell autoantibody in acquired agammaglobulinemia. J Clin Invest. 1981 Jan;67(1):42–50. doi: 10.1172/JCI110031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Saiki O., Ralph P., Cunningham-Rundles C., Good R. A. Three distinct stages of B-cell defects in common varied immunodeficiency. Proc Natl Acad Sci U S A. 1982 Oct;79(19):6008–6012. doi: 10.1073/pnas.79.19.6008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schwartz S. A., Choi Y. S., Shou L., Good R. A. Modulatory effects on immunoglobulin synthesis and secretion by lymphocytes from immunodeficient patients. J Clin Invest. 1977 Jun;59(6):1176–1187. doi: 10.1172/JCI108742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Siegal F. P., Siegal M., Good R. A. Role of helper, suppressor and B-cell defects in the pathogenesis of the hypogammaglobulinemias. N Engl J Med. 1978 Jul 27;299(4):172–178. doi: 10.1056/NEJM197807272990404. [DOI] [PubMed] [Google Scholar]
  22. Waldmann T. A., Durm M., Broder S., Blackman M., Blaese R. M., Strober W. Role of suppressor T cells in pathogenesis of common variable hypogammaglobulinaemia. Lancet. 1974 Sep 14;2(7881):609–613. doi: 10.1016/s0140-6736(74)91940-0. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES