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. 1984 Dec;53(4):623–633.

Clonal analysis of T-cell responses to herpes simplex virus: isolation, characterization and antiviral properties of an antigen-specific helper T-cell clone.

K N Leung, A A Nash, D Y Sia, P Wildy
PMCID: PMC1454870  PMID: 6209206

Abstract

A herpes simplex virus (HSV)-specific long-term T-cell clone has been established from the draining lymph node cells of BALB/c mice; the cells required repeated in vitro restimulation with UV-irradiated virus. The established T-cell clone expresses the Thy-1 and Lyt-1+2,3- surface antigens. For optimal proliferation of the cloned cells, both the presence of specific antigen and an exogenous source of T-cell growth factor are required. The proliferative response of the cloned T cells was found to be virus-specific but it did not distinguish between HSV-1 and HSV-2. Adoptive cell transfer of the cloned T cells helped primed B cells to produce anti-herpes antibodies: the response was antigen-specific and cell dose-dependent. The clone failed to produce a significant DTH reaction in vivo, but did produce high levels of macrophage-activating factor. Furthermore, the T-cell clone could protect from HSV infection, as measured by a reduction in local virus growth, and by enhanced survival following the challenge of mice with a lethal dose of virus. The mechanism(s) whereby this clone protects in vivo is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adorini L., Agarossi G., Fioravanti D., Doria G. Two distinct azobenzenearsonate-specific helper T-cell subpopulations mediate different forms of T-B cooperation. Scand J Immunol. 1983 Feb;17(2):99–108. doi: 10.1111/j.1365-3083.1983.tb00771.x. [DOI] [PubMed] [Google Scholar]
  2. Burns W., Billups L. C., Notkins A. L. Thymus dependence of viral antigens. Nature. 1975 Aug 21;256(5519):654–656. doi: 10.1038/256654a0. [DOI] [PubMed] [Google Scholar]
  3. Ertl H. C., Brown E. G., Finberg R. W. Sendai virus-specific T cell clones II. Induction of interferon production by Sendai virus-specific T helper cell clones. Eur J Immunol. 1982 Dec;12(12):1051–1053. doi: 10.1002/eji.1830121212. [DOI] [PubMed] [Google Scholar]
  4. Field H. J., Wildy P. The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice. J Hyg (Lond) 1978 Oct;81(2):267–277. doi: 10.1017/s0022172400025109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gillis S., Ferm M. M., Ou W., Smith K. A. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed] [Google Scholar]
  6. Hill T. J., Field H. J., Blyth W. A. Acute and recurrent infection with herpes simplex virus in the mouse: a model for studying latency and recurrent disease. J Gen Virol. 1975 Sep;28(3):341–353. doi: 10.1099/0022-1317-28-3-341. [DOI] [PubMed] [Google Scholar]
  7. Janeway C. A., Jr, Bert D. L., Shen F. W. Cell cooperation during in vivo anti-hapten antibody responses. V. Two synergistic Ly-1+23- helper T cells with distinctive specificities. Eur J Immunol. 1980 Apr;10(4):231–236. doi: 10.1002/eji.1830100402. [DOI] [PubMed] [Google Scholar]
  8. Kapoor A. K., Nash A. A., Wildy P., Phelan J., McLean C. S., Field H. J. Pathogenesis of herpes simplex virus in congenitally athymic mice: the relative roles of cell-mediated and humoral immunity. J Gen Virol. 1982 Jun;60(Pt 2):225–233. doi: 10.1099/0022-1317-60-2-225. [DOI] [PubMed] [Google Scholar]
  9. Keller D. M., Swierkosz J. E., Marrack P., Kappler J. W. Two types of functionally distinct, synergizing helper T cells. J Immunol. 1980 Mar;124(3):1350–1359. [PubMed] [Google Scholar]
  10. Leung K. N., Ada G. L., McKenzie I. F. Specificity, Ly phenotype, and H-2 compatibility requirements of effector cells in delayed-type hypersensitivity responses to murine influenza virus infection. J Exp Med. 1980 Apr 1;151(4):815–826. doi: 10.1084/jem.151.4.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lin Y. L., Askonas B. A. Biological properties of an influenza A virus-specific killer T cell clone. Inhibition of virus replication in vivo and induction of delayed-type hypersensitivity reactions. J Exp Med. 1981 Aug 1;154(2):225–234. doi: 10.1084/jem.154.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lu L. Y., Askonas B. A. Cross-reactivity for different type A influenza viruses of a cloned T-killer cell line. Nature. 1980 Nov 13;288(5787):164–165. doi: 10.1038/288164a0. [DOI] [PubMed] [Google Scholar]
  13. Mogensen S. C. Role of macrophages in natural resistance to virus infections. Microbiol Rev. 1979 Mar;43(1):1–26. doi: 10.1128/mr.43.1.1-26.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nash A. A., Gell P. G. Membrane phenotype of murine effector and suppressor T cells involved in delayed hypersensitivity and protective immunity to herpes simplex virus. Cell Immunol. 1983 Feb 1;75(2):348–355. doi: 10.1016/0008-8749(83)90332-5. [DOI] [PubMed] [Google Scholar]
  15. Pettersson S., Pobor G., Bandeira A., Coutinho A. Distinct helper activities control growth or maturation of B lymphocytes. Eur J Immunol. 1983 Mar;13(3):249–254. doi: 10.1002/eji.1830130314. [DOI] [PubMed] [Google Scholar]
  16. RUSSELL W. C. A sensitive and precise plaque assay for herpes virus. Nature. 1962 Sep 8;195:1028–1029. doi: 10.1038/1951028a0. [DOI] [PubMed] [Google Scholar]
  17. Rosenthal K. L., Oldstone M. B., Hengartner H., Zinkernagel R. M. Specificity of in vitro cytotoxic T cell clones directed against vesicular stomatitis virus. J Immunol. 1983 Jul;131(1):475–478. [PubMed] [Google Scholar]
  18. Schmid D. S., Rouse B. T. Cellular interactions in the cytotoxic T lymphocyte response to herpes simplex virus antigens: differential antigen activation requirements for the helper T lymphocyte and cytotoxic T lymphocyte precursors. J Immunol. 1983 Jul;131(1):479–484. [PubMed] [Google Scholar]
  19. Sethi K. K., Omata Y., Schneweis K. E. Protection of mice from fatal herpes simplex virus type 1 infection by adoptive transfer of cloned virus-specific and H-2-restricted cytotoxic T lymphocytes. J Gen Virol. 1983 Feb;64(Pt 2):443–447. doi: 10.1099/0022-1317-64-2-443. [DOI] [PubMed] [Google Scholar]
  20. Sia D. Y., Lachmann P. J., Leung K. N. Studies in the enhancement of tumour immunity by coupling strong antigens to tumour cells ('heterogenization of tumours'). Helper T cell clones against PPD help other T cells mount anti-tumour responses to PPD-coupled tumour cells. Immunology. 1984 Apr;51(4):755–763. [PMC free article] [PubMed] [Google Scholar]
  21. Smith K. A., Ruscetti F. W. T-cell growth factor and the culture of cloned functional T cells. Adv Immunol. 1981;31:137–175. doi: 10.1016/s0065-2776(08)60920-7. [DOI] [PubMed] [Google Scholar]
  22. Zinkernagel R. M., Doherty P. C. MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness. Adv Immunol. 1979;27:51–177. doi: 10.1016/s0065-2776(08)60262-x. [DOI] [PubMed] [Google Scholar]

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