Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1984 Dec;53(4):783–790.

In vitro synthesis of IgE by human lymphocytes. IV. Suppression of the spontaneous IgE synthesis by IgE-binding factors secreted by tunicamycin-treated RPMI 8866 cells.

M Sarfati, E Rector, A H Sehon, G Delespesse
PMCID: PMC1454884  PMID: 6238905

Abstract

It was previously shown that RPMI 8866 cells released IgE-binding factors (IgE-BFs) capable of enhancing the spontaneous in vitro synthesis of IgE by purified B lymphocytes isolated from allergic individuals. In the present study, the influence of tunicamycin, an inhibitor of protein glycosylation, on RPMI 8866 cells was investigated with regard to: (i) the expression of surface receptors for IgE; (ii) the release of IgE-BFs into the culture supernatants, and (iii) the biological activity of IgE-BFs. After preincubation for 60 min with tunicamycin (1 microgram/ml), RPMI 8866 cells were cultured for 48 hr in HB 101 serum-free medium; the culture supernatant was then filtered, concentrated, and its biological activity was compared to that of a parallel culture supernatant from untreated RPMI 8866 cells. The results of these experiments indicate that exposure of RPMI 8866 cells to tunicamycin resulted in: (i) a reduction of surface Fc epsilon R; (ii) no effect on the release of IgE-BFs into the culture supernatant, and (iii) the conversion of IgE-potentiating factors into IgE-suppressing factors. The latter factors suppressed the IgE secretion by U266 myeloma cells and completely inhibited the activity of IgE-potentiating factors on B lymphocytes from allergic individuals. IgE-BFs secreted by tunicamycin-treated cells had no effect on the production of IgG, IgA or IgM by normal or EBV-transformed B cells.

Full text

PDF
783

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Böyum A. Separation of leukocytes from blood and bone marrow. Introduction. Scand J Clin Lab Invest Suppl. 1968;97:7–7. [PubMed] [Google Scholar]
  2. Conrad D. H., Froese A. Characterization of the target cell receptor for IgE. III. properties of the receptor isolated from rat basophilic leukemia cells by affinity chromatography. J Immunol. 1978 Feb;120(2):429–437. [PubMed] [Google Scholar]
  3. Deguchi H., Suemura M., Ishizaka A., Ozaki Y., Kishimoto S., Yamamura Y., Kishimoto T. IgE class-specific suppressor T cells and factors in humans. J Immunol. 1983 Dec;131(6):2751–2756. [PubMed] [Google Scholar]
  4. Huff T. F., Ishizaka K. Formation of IgE-binding factors by human T-cell hybridomas. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1514–1518. doi: 10.1073/pnas.81.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Huff T. F., Uede T., Iwata M., Ishizaka K. Modulation of the biologic activities of IgE-binding factors. III. Switching of a T cell hybrid clone from the formation of IgE-suppressive factor to the formation of IgE-potentiating factor. J Immunol. 1983 Sep;131(3):1090–1095. [PubMed] [Google Scholar]
  6. Ishizaka K., Sandberg K. Formation of IgE binding factors by human T lymphocytes. J Immunol. 1981 May;126(5):1692–1696. [PubMed] [Google Scholar]
  7. Kaplan M. E., Clark C. An improved rosetting assay for detection of human T lymphocytes. J Immunol Methods. 1974 Jul;5(2):131–135. doi: 10.1016/0022-1759(74)90003-9. [DOI] [PubMed] [Google Scholar]
  8. Milburn G. L., Lynch R. G. Immunoregulation of murine myeloma in vitro. II. Suppression of MOPC-315 immunoglobulin secretion and synthesis by idiotype-specific suppressor T cells. J Exp Med. 1982 Mar 1;155(3):852–862. doi: 10.1084/jem.155.3.852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mosier D. E., Coppleson L. W. A THREE-CELL INTERACTION REQUIRED FOR THE INDUCTION OF THE PRIMARY IMMUNE RESPONSE in vitro. Proc Natl Acad Sci U S A. 1968 Oct;61(2):542–547. doi: 10.1073/pnas.61.2.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Romagnani S., Maggi E., Del Prete G. F., Troncone R., Ricci M. In vitro production of IgE by human peripheral blood mononuclear cells. I. Rate of IgE biosynthesis. Clin Exp Immunol. 1980 Oct;42(1):167–174. [PMC free article] [PubMed] [Google Scholar]
  11. Sarfati M., Rector E., Rubio-Trujillo M., Wong K., Sehon A. H., Delespesse G. In vitro synthesis of IgE by human lymphocytes. III. IgE-potentiating activity of culture supernatants from Epstein-Barr virus (EBV) transformed B cells. Immunology. 1984 Oct;53(2):207–214. [PMC free article] [PubMed] [Google Scholar]
  12. Sarfati M., Rector E., Wong K., Rubio-Trujillo M., Sehon A. H., Delespesse G. In vitro synthesis of IgE by human lymphocytes. II. Enhancement of the spontaneous IgE synthesis by IgE-binding factors secreted by RPMI 8866 lymphoblastoid B cells. Immunology. 1984 Oct;53(2):197–205. [PMC free article] [PubMed] [Google Scholar]
  13. Sarfati M., Rubio-Trujillo M., Wong K., Rector E., Sehon A. H., Delespesse G. In vitro synthesis of IgE by human lymphocytes. I. The spontaneous secretion of IgE by B lymphocytes from allergic individuals: a model to investigate the regulation of human IgE synthesis. Immunology. 1984 Oct;53(2):187–196. [PMC free article] [PubMed] [Google Scholar]
  14. Saxon A., Stevens R. H. Stimulation and regulation of human IgE production in vitro using peripheral blood lymphocytes. Clin Immunol Immunopathol. 1979 Dec;14(4):474–488. doi: 10.1016/0090-1229(79)90100-4. [DOI] [PubMed] [Google Scholar]
  15. Spiegelberg H. L. Lymphocytes bearing Fc receptors for IgE. Immunol Rev. 1981;56:199–218. doi: 10.1111/j.1600-065x.1981.tb01052.x. [DOI] [PubMed] [Google Scholar]
  16. Struck D. K., Lennarz W. J. Evidence for the participation of saccharide-lipids in the synthesis of the oligosaccharide chain of ovalbumin. J Biol Chem. 1977 Feb 10;252(3):1007–1013. [PubMed] [Google Scholar]
  17. Suemura M., Ishizaka A., Kobatake S., Sugimura K., Maeda K., Nakanishi K., Kishimoto S., Yamamura Y., Kishimoto T. Inhibition of IgE production in B hybridomas by IgE class-specific suppressor factor from T hybridomas. J Immunol. 1983 Mar;130(3):1056–1060. [PubMed] [Google Scholar]
  18. Yodoi J., Hirashima M., Ishizaka K. Lymphocytes bearing Fc receptors for IgE. V. Effect of tunicamycin on the formation of IgE-potentiating factor and IgE-suppressive factor by con A-activated lymphocytes. J Immunol. 1981 Mar;126(3):877–882. [PubMed] [Google Scholar]
  19. Yodoi J., Hirashima M., Ishizaka K. Regulatory role of IgE-binding factors from rat T lymphocytes. V. The carbohydrate moieties in IgE-potentiating factors and IgE-suppressive factors. J Immunol. 1982 Jan;128(1):289–295. [PubMed] [Google Scholar]
  20. Young N. M., Leon M. A. The affinity of concanavalin A and Lens culinaris hemagglutinin for glycopeptides. Biochim Biophys Acta. 1974 Oct 9;365(2):418–424. doi: 10.1016/0005-2795(74)90015-4. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES