Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1984 Dec;53(4):769–777.

Neoplasms arising in CBA mice after transfer of spleen cells from syngeneic old donors.

V J Wallis, M Chaudhuri, M C Jacob, B A Valkova, A J Davies
PMCID: PMC1454902  PMID: 6334020

Abstract

Young (15-week-old) CBA/Ca mice were injected intravenously with spleen cells from individual young (15-week-old) or old (18-24-month-old) CBA/T6T6 mice. Samples of peripheral blood were taken at monthly intervals and cultured with phytohaemagglutinin (PHA) to stimulate T lymphocytes into mitosis. In the recipients of young lymphocytes, the percentage of donor cells dividing in these cultures remained low throughout the experiment, but in the recipients of old spleen cells, after an initial period when the percentage of donor cells declined, there was a marked increase in the percentage of donor cells. The interval between the injection and the increase in the proportion of donor cells was very variable. Ten of the 12 recipients of old lymphocytes developed tumours involving the spleen and mesenteric lymph node. They resembled type B reticulum cell neoplasms as described by Dunn & Deringer (1968), and all but two were transplantable. In addition, one mouse that had no evidence of tumour on histological examination nevertheless gave rise to a transplantable tumour. The five tumours on which chromosomal analysis was carried out proved to be of old donor cell origin. Two out of the five recipients of young cells also eventually developed tumours, but these arose later than the others, had a more granulocytic character, and did not transplant.

Full text

PDF
769

Images in this article

774Figure 2
on p.774

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baird S. M., Beattie G. M., Lannom R. A., Lipsick J. S., Jensen F. C., Kaplan N. O. Induction of lymphoma in antigenically stimulated athymic mice. Cancer Res. 1982 Jan;42(1):198–206. [PubMed] [Google Scholar]
  2. Chaudhuri M., Wallis V. J., Connell D. I. Recirculating and sessile B cell populations in normal and CBA/N mice. Eur J Immunol. 1983 Oct;13(10):789–793. doi: 10.1002/eji.1830131002. [DOI] [PubMed] [Google Scholar]
  3. Doenhoff M. J., Janossy G., Greaves M. F., Gomer K. J., Snajdr J. Lymphocyte activation. VI. A re-evaluation of factors affecting the selectivity of polyclonal mitogens for mouse T and B cells. Clin Exp Immunol. 1974 Jul;17(3):475–490. [PMC free article] [PubMed] [Google Scholar]
  4. Dunn T. B., Deringer M. K. Reticulum cell neoplasm, type B, or the "Hodgkin's-like lesion" of the mouse. J Natl Cancer Inst. 1968 Apr;40(4):771–821. [PubMed] [Google Scholar]
  5. Ebbesen P. Reticulosarcoma and amyloid development in BALB/c mice inoculated with syngeneic cells from young and old donors. J Natl Cancer Inst. 1971 Dec;47(6):1241–1245. [PubMed] [Google Scholar]
  6. Gleichmann E., Gleichmann H., Wilke W. Autoimmunization and lymphomagenesis in parent to F1 combinations differing at the major histocompatibility complex: model for spontaneous disease caused by altered self-antigens? Transplant Rev. 1976;31:156–224. doi: 10.1111/j.1600-065x.1976.tb01454.x. [DOI] [PubMed] [Google Scholar]
  7. Gozes Y., Umiel T., Meshorer A., Trainin N. Syngeneic GvH induced in popliteal lymph nodes by spleen cells of old C57BL/6 mice. J Immunol. 1978 Dec;121(6):2199–2204. [PubMed] [Google Scholar]
  8. HARRIS J. E., FORD C. E. CELLULAR TRAFFIC OF THE THYMUS: EXPERIMENTS WITH CHROMOSOME MARKERS. EVIDENCE THAT THE THYMUS PLAYS AN INSTRUCTIONAL PART. Nature. 1964 Feb 29;201:884–885. doi: 10.1038/201884a0. [DOI] [PubMed] [Google Scholar]
  9. Krueger G. R., Malmgren R. A., Berard C. W. Malignant lymphomas and plasmacytosis in mice under prolonged immunosuppression and persistent antigenic stimulation. Transplantation. 1971 Feb;11(2):138–144. doi: 10.1097/00007890-197102000-00006. [DOI] [PubMed] [Google Scholar]
  10. Lanier L. L., Lynes M., Haughton G., Wettstein P. J. Novel type of murine B-cell lymphoma. Nature. 1978 Feb 9;271(5645):554–555. doi: 10.1038/271554a0. [DOI] [PubMed] [Google Scholar]
  11. METCALF D. Reticular tumours in mice subjected to prolonged antigenic stimulation. Br J Cancer. 1961 Dec;15:769–779. doi: 10.1038/bjc.1961.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sharp J. G., Riches A. C., Littlewood V., Thomas D. B. The incidence, pathology and transplantation of hepatomas in CBA mice. J Pathol. 1976 Aug;119(4):211–220. doi: 10.1002/path.1711190405. [DOI] [PubMed] [Google Scholar]
  13. Stutman O. Immunodepression and malignancy. Adv Cancer Res. 1975;22:261–422. doi: 10.1016/s0065-230x(08)60179-7. [DOI] [PubMed] [Google Scholar]
  14. Wallis V. J., Leuchars E., Chaudhuri M., Davies A. J. Studies on hyperlymphoid mice. Immunology. 1979 Sep;38(1):163–171. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES