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. 1984 Nov;53(3):465–472.

Cell-mediated immunity in experimental murine dermatophytosis. II. Adoptive transfer of immunity to dermatophyte infection by lymphoid cells from donors with acute or chronic infections.

R A Calderon, R J Hay
PMCID: PMC1454912  PMID: 6333384

Abstract

An in vivo adoptive cell transfer system was used to investigate protective immunity during experimental murine dermatophytosis due to Trichophyton quinckeanum. Using sublethally (550R) irradiated BALB/c mice which are particularly susceptible to infection, it was found that regional lymph node cells from syngeneic acutely-infected donors conferred protection to irradiated recipients. By contrast, serum from the same donors did not prevent infection of normal BALB/c mice. The capacity of lymphoid cells to transfer adoptive immunity was lost after treatment with monoclonal anti-Thy-1.2 antibody and complement, but not after treatment with monoclonal anti-Ly 2.2 antibody and complement, indicating that cellular immunity is mediated by Ly 2-T cells. The cellular activity of chronically infected mice was also studied. Paradoxically, lymphoid cells from these animals which appeared to be immunologically compromised did, however, confer immunity. Adoptive immunity was abrogated by serum from chronically infected donors or by specific dermatophyte antigen, but not by homologous or heterologous anti-dermatophyte antiserum. These findings indicate that competition between cell-mediated immunity and humoral factors may be the causative mechanism for persistence of dermatophyte infection in this system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen D. E., Snyderman R., Meadows L., Pinnell S. R. Generalized microsporum audoninii infection and depressed cellular immunity associated with a missing plasma factor required for lymphocyte blastogenesis. Am J Med. 1977 Dec;63(6):991–1000. doi: 10.1016/0002-9343(77)90554-x. [DOI] [PubMed] [Google Scholar]
  2. Asherson G. L. Antigen-mediated depression of delayed hypersensitivity. Br Med Bull. 1967 Jan;23(1):24–29. doi: 10.1093/oxfordjournals.bmb.a070510. [DOI] [PubMed] [Google Scholar]
  3. BONK A. F., FRIEDMAN L., DERBES V. J. Experimental dermatophytosis. J Invest Dermatol. 1962 Oct;39:281–286. doi: 10.1038/jid.1962.113. [DOI] [PubMed] [Google Scholar]
  4. Calderon R. A., Hay R. J. Cell-mediated immunity in experimental murine dermatophytosis. I. Temporal aspects of T-suppressor activity caused by Trichophyton quinckeanum. Immunology. 1984 Nov;53(3):457–464. [PMC free article] [PubMed] [Google Scholar]
  5. Cantor H., Boyse E. A. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975 Jun 1;141(6):1376–1389. doi: 10.1084/jem.141.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dwyer J. M., Kantor F. S. Regulation of delayed hypersensitivity. Failure to transfer delayed hypersensitivity to desensitized guinea pigs. J Exp Med. 1973 Jan 1;137(1):32–41. doi: 10.1084/jem.137.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grappel S. F., Bishop C. T., Blank F. Immunology of dermatophytes and dermatophytosis. Bacteriol Rev. 1974 Jun;38(2):222–250. doi: 10.1128/br.38.2.222-250.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Green F., 3rd, Balish E. Suppression of in vitro lymphocyte transformation during an experimental dermatophyte infection. Infect Immun. 1979 Nov;26(2):554–562. doi: 10.1128/iai.26.2.554-562.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hanifin J. M., Ray L. F., Lobitz W. C., Jr Immunological reactivity in dermatophytosis. Br J Dermatol. 1974 Jan;90(1):1–8. [PubMed] [Google Scholar]
  10. Hay R. J., Calderon R. A., Collins M. J. Experimental dermatophytosis: the clinical and histopathologic features of a mouse model using Trichophyton quinckeanum (mouse favus). J Invest Dermatol. 1983 Sep;81(3):270–274. doi: 10.1111/1523-1747.ep12518292. [DOI] [PubMed] [Google Scholar]
  11. Hay R. J., Shennan G. Chronic dermatophyte infections II. Antibody and cell-mediated immune responses. Br J Dermatol. 1982 Feb;106(2):191–198. doi: 10.1111/j.1365-2133.1982.tb00928.x. [DOI] [PubMed] [Google Scholar]
  12. Haynes B. F., Hensley L. L., Jegasothy B. V. Differentiation of human T lymphocytes: II. Phenotypic difference in skin and blood malignant T-cells in cutaneous T-cell lymphoma. J Invest Dermatol. 1982 Apr;78(4):323–326. doi: 10.1111/1523-1747.ep12507406. [DOI] [PubMed] [Google Scholar]
  13. Hellström K. E., Hellström I. Lymphocyte-mediated cytotoxicity and blocking serum activity to tumor antigens. Adv Immunol. 1974;18:209–277. doi: 10.1016/s0065-2776(08)60311-9. [DOI] [PubMed] [Google Scholar]
  14. Jones H. E., Reinhardt J. H., Rinaldi M. G. Acquired immunity to dermatophytes. Arch Dermatol. 1974 Jun;109(6):840–848. [PubMed] [Google Scholar]
  15. Jones H. E., Reinhardt J. H., Rinaldi M. G. Immunologic susceptibility to chronic dermatophytosis. Arch Dermatol. 1974 Aug;110(2):213–220. [PubMed] [Google Scholar]
  16. Jones H. E., Reinhardt J. H., Rinaldi M. G. Model dermatophytosis in naturally infected subjects. Arch Dermatol. 1974 Sep;110(3):369–374. [PubMed] [Google Scholar]
  17. Knight A. G. A review of experimental human fungus infections. J Invest Dermatol. 1972 Oct;59(4):354–358. doi: 10.1111/1523-1747.ep12627413. [DOI] [PubMed] [Google Scholar]
  18. Kung P. C., Berger C. L., Goldstein G., LoGerfo P., Edelson R. L. Cutaneous T cell lymphoma: characterization by monoclonal antibodies. Blood. 1981 Feb;57(2):261–266. [PubMed] [Google Scholar]
  19. Patterson J. A., Edelson R. L. Interaction of T cells with the epidermis. Br J Dermatol. 1982 Jul;107(1):117–122. doi: 10.1111/j.1365-2133.1982.tb00299.x. [DOI] [PubMed] [Google Scholar]
  20. Petts V., Roitt I. M. Peroxidase conjugates for demonstration of tissue antibodies: evaluation of the technique. Clin Exp Immunol. 1971 Sep;9(3):407–418. [PMC free article] [PubMed] [Google Scholar]
  21. Walters B. A., Chick J. E., Halliday W. J. Cell-mediated immunity and serum blocking factors in patients with chronic dermatophytic infections. Int Arch Allergy Appl Immunol. 1974;46(6):849–857. doi: 10.1159/000231187. [DOI] [PubMed] [Google Scholar]
  22. Yoshida T., Benacerraf B., McCluskey R. T., Vassalli P. The effect of intravenous antigen on circulating monocytes in animals with delayed hypersensitivity. J Immunol. 1969 Apr;102(4):804–811. [PubMed] [Google Scholar]

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