Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1984 Nov;53(3):553–561.

Abnormalities of third-order suppressor T cells in old (New Zealand black x New Zealand white) F1 mice.

K Okuda, S Nagaoka, K Katoh, K Matsunaga, Y Ishigatubo, M Minami, K Tani
PMCID: PMC1454922  PMID: 6208147

Abstract

The suppressor T cell (Ts) function of old NZW, NZB, C57BL/6 and (NZB X NZW) F1 [B/W)F1), mice to the 2,4-dinitro-1-fluorobenzene (DNFB) hapten was studied. Intravenous administration of dinitrophenyl (DNP) coupled syngeneic lymphoid cells (which normally induce DNP specific suppression) did not result in suppression of DNFB-specific contact hypersensitivity (CS) responses in old NZB or (B/W) F1 mice. Nevertheless, when spleen cells from these old mice were injected into young mice (either (B/W)F1 or A/Sn), strong suppression of the induction phase of CS responses was observed. In addition, effector phase suppressor activity was also observed when splenic cells from tolerized old (B/W) F1 donors were transferred into young (B/W)F1 mice during the effector phase of the CS response. In both cases, the significant cells in the transfer were I-J+ T cells. Thus, the old mice retained functional Ts1 and Ts2 suppressor cells. However, the suppressive activity of the old mice could be reconstituted with spleen cells from primed young mice, suggesting that they have a defect in the Ts3 subset. This was further supported by the finding that the significant cells from the primed young mice were I-J positive and cyclophosphamide-sensitive.

Full text

PDF
553

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barthold D. R., Kysela S., Steinberg A. D. Decline in suppressor T cell function with age in female NZB mice. J Immunol. 1974 Jan;112(1):9–16. [PubMed] [Google Scholar]
  2. Cantor H., McVay-Boudreau L., Hugenberger J., Naidorf K., Shen F. W., Gershon R. K. Immunoregulatory circuits among T-cell sets. II. Physiologic role of feedback inhibition in vivo: absence in NZB mice. J Exp Med. 1978 Apr 1;147(4):1116–1125. doi: 10.1084/jem.147.4.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Claman H. N., Miller S. D., Sy M. S., Moorhead J. W. Suppressive mechanisms involving sensitization and tolerance in contact allergy. Immunol Rev. 1980;50:105–132. doi: 10.1111/j.1600-065x.1980.tb00309.x. [DOI] [PubMed] [Google Scholar]
  4. Dietz M. H., Sy M. S., Benacerraf B., Nisonoff A., Greene M. I., Germain R. N. Antigen- and receptor-driven regulatory mechanisms. VII. H-2-restricted anti-idiotypic suppressor factor from efferent suppressor T cells. J Exp Med. 1981 Feb 1;153(2):450–463. doi: 10.1084/jem.153.2.450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dietz M. H., Sy M. S., Greene M. I., Nisonoff A., Benacerraf B., Germain R. N. Antigen and receptor-driven regulatory mechanisms. VI. Demonstration of cross-reactive idiotypic determinants on azobenzenearsonate-specific antigen-binding suppressor T cells producing soluble suppressor factor(s). J Immunol. 1980 Dec;125(6):2374–2379. [PubMed] [Google Scholar]
  6. Gershwin M. E., Castles J. J., Erickson K., Ahmed A. Studies of congenitally immunologic mutant New Zealand mice. II. Absence of T cell progenitor populations and B cell defects of congenitally athymic (nude) New Zealand Black (NZB) mice. J Immunol. 1979 May;122(5):2020–2025. [PubMed] [Google Scholar]
  7. Izui S., McConahey P. J., Dixon F. J. Increased spontaneous polyclonal activation of B lymphocytes in mice with spontaneous autoimmune disease. J Immunol. 1978 Dec;121(6):2213–2219. [PubMed] [Google Scholar]
  8. Laskin C. A., Smathers P. A., Reeves J. P., Steinberg A. D. Studies of defective tolerance induction in NZB mice. Evidence for a marrow pre-T cell defect. J Exp Med. 1982 Apr 1;155(4):1025–1036. doi: 10.1084/jem.155.4.1025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Laskin C. A., Taurog J. D., Smathers P. A., Steinberg A. D. Studies of defective tolerance in murine lupus. J Immunol. 1981 Nov;127(5):1743–1747. [PubMed] [Google Scholar]
  10. Miller S. D., Claman H. N. The induction of hapten-specific T cell tolerance by using hapten-modified lymphoid cells. I. Characteristics of tolerance induction. J Immunol. 1976 Nov;117(5 Pt 1):1519–1526. [PubMed] [Google Scholar]
  11. Miller S. D. Suppressor T-cell mechanisms in contact sensitivity. III. Apparent non-major histocompatibility complex restriction is a result of multiple sets of major histocompatibility complex-specific suppressor T cells induced by syngeneic 2,4-dinitrophenyl-modified lymphoid cells. J Exp Med. 1979 Sep 19;150(3):676–692. doi: 10.1084/jem.150.3.676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miller S. D., Sy M. S., Claman H. N. Suppressor T cell mechanisms in contact sensitivity. II. Afferent blockade by alloinduced suppressor T cells. J Immunol. 1978 Jul;121(1):274–280. [PubMed] [Google Scholar]
  13. Minami M., Honji N., Dorf M. E. Mechanism responsible for the induction of I-J restriction on TS3 suppressor cells. J Exp Med. 1982 Nov 1;156(5):1502–1515. doi: 10.1084/jem.156.5.1502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Minami M., Okuda K., Furusawa S., Benacerraf B., Dorf M. E. Analysis of T cell hybridomas. I. Characterization of H-2 and Igh-restricted monoclonal suppressor factors. J Exp Med. 1981 Nov 1;154(5):1390–1402. doi: 10.1084/jem.154.5.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Okuda K., Minami M., Furusawa M., Dorf M. E. Analysis of T cell hybridomas. II. Comparisons among three distinct types of monoclonal suppressor factors. J Exp Med. 1981 Dec 1;154(6):1838–1851. doi: 10.1084/jem.154.6.1838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Okuda K., Minami M., Ju S. T., Dorf M. E. Functional association of idiotypic and I-J determinants on the antigen receptor of suppressor T cells. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4557–4561. doi: 10.1073/pnas.78.7.4557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Okuda K., Minami M., Sherr D. H., Dorf M. E. Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. XI. Pseudogenetic restrictions of hybridoma suppressor factors. J Exp Med. 1981 Aug 1;154(2):468–479. doi: 10.1084/jem.154.2.468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sherr D. H., Dorf M. E. Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. IX. Characterization of Idiotype-specific effector-phase suppressor cells on plaque-forming cell responses in vitro. J Exp Med. 1981 Jun 1;153(6):1445–1456. doi: 10.1084/jem.153.6.1445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sunday M. E., Benacerraf B., Dorf M. E. Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. VIII. Suppressor cell pathways in cutaneous sensitivity responses. J Exp Med. 1981 Apr 1;153(4):811–822. doi: 10.1084/jem.153.4.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sy M. S., Miller S. D., Moorhead J. W., Claman H. N. Active suppression of 1-fluoro-2,4-dinitrobenzene-immune T cells. Requirement of an auxiliary T cell induced by antigen. J Exp Med. 1979 May 1;149(5):1197–1207. doi: 10.1084/jem.149.5.1197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Theofilopoulos A. N., Dixon F. J. Etiopathogenesis of murine SLE. Immunol Rev. 1981;55:179–216. doi: 10.1111/j.1600-065x.1981.tb00343.x. [DOI] [PubMed] [Google Scholar]
  22. Weinberger J. Z., Germain R. N., Benacerraf B., Dorf M. E. Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. V. Role of idiotypes in the suppressor pathway. J Exp Med. 1980 Jul 1;152(1):161–169. doi: 10.1084/jem.152.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Zembala M. A., Asherson G. L., James B. M., Stein V. E., Watkins M. C. Anti-haptene T suppressor factor acts through an I-J+, Ly1-2+, T acceptor cell that releases a nonspecific inhibitor of the transfer of contact sensitivity when exposed to antigen. J Immunol. 1982 Nov;129(5):1823–1829. [PubMed] [Google Scholar]
  24. Zembala M., Asherson G. L., Colizzi V., Watkins M. C. Desensitization in vitro: the role of T-suppressor cells, T-suppressor factor and T-acceptor cells in the inhibition of the passive transfer of contact sensitivity to picryl chloride by exposure to antigen in vitro. Immunology. 1982 Dec;47(4):605–615. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES