Abstract
Concentrations of total colostral IgA were compared with concentrations remaining after precipitation with antibodies to secretory piece (SP). The anti-SP was obtained by filtering anti-colostral IgA through an immunosorbent column with firmly attached human serum globulins. IgA concentrations were measured by radial immunodiffusion with anti-α-chain serum.
The proportions of SP-containing IgA molecules were: in whole (defatted) colostrum, at least 68 per cent; in the polymeric IgA fraction, at least 88 per cent; in the monomeric IgA fraction, 43 per cent. Free SP was also detected in colostrum. The non-identity of SP with several known components of colostrum was ascertained by blocking experiments.
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