Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1970 Jul;19(1):55–63.

Experimental autoimmune encephalomyelitis*

Digestion of basic protein of human myelin with cyanogen bromide and trypsin

P R Carnegie, T A McPherson, G S M Robson
PMCID: PMC1455612  PMID: 4098596

Abstract

From a cyanogen bromide digest of the encephalitogenic basic protein of human brain a polypeptide—derived from the N-terminal end of the protein—was isolated and shown to induce experimental autoimmune encephalomyelitis. At low doses this peptide induced typical histological lesions unaccompanied by clinical symptoms and humoral antibody. Digestion of the protein with trypsin did not destroy its encephalitogenic activity. The basic protein of human myelin contains more than one encephalitogenic determinant.

Full text

PDF
55

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CASPARY E. A., FIELD E. J. AN ENCEPHALITOGENIC PROTEIN OF HUMAN ORIGIN: SOME CHEMICAL AND BIOLOGICAL PROPERTIES. Ann N Y Acad Sci. 1965 Mar 31;122:182–198. doi: 10.1111/j.1749-6632.1965.tb20202.x. [DOI] [PubMed] [Google Scholar]
  2. Carnegie P. R. A peptide-mapping technique for the estimation of molecular size. Nature. 1965 Jun 12;206(989):1128–1130. doi: 10.1038/2061128a0. [DOI] [PubMed] [Google Scholar]
  3. Carnegie P. R., Bencina B., Lamoureux G. Experimental allergic encephalomyelitis. Isolation of basic proteins and polypeptides from central nervous tissue. Biochem J. 1967 Nov;105(2):559–568. doi: 10.1042/bj1050559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carnegie P. R. Digestion of an arg-pro bond by trypsin in the ecephalitogenic basic protein of human myelin. Nature. 1969 Aug 30;223(5209):958–959. doi: 10.1038/223958a0. [DOI] [PubMed] [Google Scholar]
  5. Carnegie P. R., Lamoureux G., Bencina B. Proposed technique for classifying and identifying encephalitogens. Nature. 1967 Apr 22;214(5086):407–408. doi: 10.1038/214407a0. [DOI] [PubMed] [Google Scholar]
  6. Carnegie P. R., Lumsden C. E. Fractionation of encephalitogenic polypeptides from bovine spinal cord by gel filtration in phenol--acetic acid--water. Immunology. 1967 Feb;12(2):133–145. [PMC free article] [PubMed] [Google Scholar]
  7. Carnegie P. R. N-terminal sequence of an encephalitogenic protein form human myelin. Biochem J. 1969 Jan;111(2):240–242. doi: 10.1042/bj1110240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Chao L. P., Einstein E. R. Isolation and characterization of an active fragment from enzymatic degradation of encephalitogenic protein. J Biol Chem. 1968 Nov 25;243(22):6050–6055. [PubMed] [Google Scholar]
  9. Eng L. F., Chao F. C., Gerstl B., Pratt D., Tavaststjerna M. G. The maturation of human white matter myelin. Fractionation of the myelin membrane proteins. Biochemistry. 1968 Dec;7(12):4455–4465. doi: 10.1021/bi00852a042. [DOI] [PubMed] [Google Scholar]
  10. Eylar E. H., Hashim G. A. Allergic encephalomyelitis: the structure of encephalitogenic determinant. Proc Natl Acad Sci U S A. 1968 Oct;61(2):644–650. doi: 10.1073/pnas.61.2.644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Eylar E. H., Thompson M. Allergic encephalomyelitis: the physico-chemical properities of the basic protein encephalitogen from bovine spinal cord. Arch Biochem Biophys. 1969 Feb;129(2):468–479. doi: 10.1016/0003-9861(69)90204-5. [DOI] [PubMed] [Google Scholar]
  12. Hashim G. A., Eylar E. H. Allergic encephalomyelitis: enzymatic degradation of the encephalitogenic basic protein from bovine spinal cord. Arch Biochem Biophys. 1969 Feb;129(2):635–644. doi: 10.1016/0003-9861(69)90224-0. [DOI] [PubMed] [Google Scholar]
  13. Hashim G. A., Eylar E. H. The structure of the terminal regions of the encephalitogenic A1 protein. Biochem Biophys Res Commun. 1969 Mar 31;34(6):770–776. doi: 10.1016/0006-291x(69)90246-0. [DOI] [PubMed] [Google Scholar]
  14. Kibler R. F., Shapira R., McKneally S., Jenkins J., Selden P., Chou F. Encephalitogenic protein: structure. Science. 1969 May 2;164(3879):577–580. doi: 10.1126/science.164.3879.577. [DOI] [PubMed] [Google Scholar]
  15. Lamoureux G., Carnegie P. R., McPherson T. A., Johnston D. Experimental allergic encephalomyelitis: immunological properties of encephalitogenic polypeptides and proteins. Clin Exp Immunol. 1967 Sep;2(5):601–609. [PMC free article] [PubMed] [Google Scholar]
  16. Lisak R. P., Heinze R. G., Kies M. W., Alvord E. C., Jr Antibodies to encephalitogenic basic protein in experimental allergic encephalomyelitis. Proc Soc Exp Biol Med. 1969 Mar;130(3):814–818. doi: 10.3181/00379727-130-33662. [DOI] [PubMed] [Google Scholar]
  17. Lumsden C. E., Robertson D. M., Blight R. Chemical studies on experimental allergic encephalomyelitis. Peptide as the common denominator in all encephalitogenic 'antigens'. J Neurochem. 1966 Mar;13(3):127–162. doi: 10.1111/j.1471-4159.1966.tb07507.x. [DOI] [PubMed] [Google Scholar]
  18. McPherson T. A., Carnegie P. R. Radioimmunoassay with gel filtration for detecting antibody to basic proteins of myelin. J Lab Clin Med. 1968 Nov;72(5):824–831. [PubMed] [Google Scholar]
  19. Offord R. E. Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature. 1966 Aug 6;211(5049):591–593. doi: 10.1038/211591a0. [DOI] [PubMed] [Google Scholar]
  20. Palmer F. B., Dawson R. M. The isolation and properties of experimental allergic encephalitogenic protein. Biochem J. 1969 Mar;111(5):629–636. doi: 10.1042/bj1110629. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES