Abstract
Sheep erythrocytes sensitized with rabbit γG anti-Forssman haemolysin may be haemolysed more efficiently and to a greater extent by limited amounts of guinea-pig complement components than are cells sensitized with γM anti-Forssman haemolysin. This apparent difference, noted in all components titrations, was two-fold or greater in titrations of C2 and components following C2 in the haemolytic sequence. Neither site to site transfer of C2 nor AC142 could account for the 100 per cent greater haemolytic efficiency of C2 in haemolytic assays. These results indicate that immunoglobulin class plays a more complex role in immune haemolysis than simple initiation of complement fixation and suggest that γG complement fixing sites may be more efficient than γM sites in producing biological damage.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BORSOS T., RAPP H. J. CHROMATOGRAPHIC SEPARATION OF THE FIRST COMPONENT OF COMPLEMENT AND ITS ASSAY ON A MOLECULAR BASIS. J Immunol. 1963 Dec;91:851–858. [PubMed] [Google Scholar]
- Borsos T., Rapp H. J. Immune hemolysis: a simplified method for the preparation of EAC'4 with guinea pig or with human complement. J Immunol. 1967 Aug;99(2):263–268. [PubMed] [Google Scholar]
- Colten H. R., Borsos T., Rapp H. J. Titration of the first component of complement on a molecular basis: suitability of IgM and unsuitability of IgG hemolysins as sensitizer. Immunochemistry. 1969 May;6(3):461–467. doi: 10.1016/0019-2791(69)90302-4. [DOI] [PubMed] [Google Scholar]
- Connell R. S., Bacon R. L. Nucleoside phosphatases of fetal and maternal blood cells: electron microscope study. Science. 1965 Oct 22;150(3695):503–504. doi: 10.1126/science.150.3695.503. [DOI] [PubMed] [Google Scholar]
- Frank M. M., Dourmashkin R. R., Humphrey J. H. Observations on the mechanism of immune hemolysis: importance of immunoglobulin class and source of complement on the extent of damage. J Immunol. 1970 Jun;104(6):1502–1510. [PubMed] [Google Scholar]
- Frank M. M., Gaither T. The effect of temperature on the reactivity of guinea-pig complement with gamma G and gamma M haemolytic antibodies. Immunology. 1970 Dec;19(6):967–974. [PMC free article] [PubMed] [Google Scholar]
- Frank M. M., Humphrey J. H. The subunits in rabbit anti-Forssman IgM antibody. J Exp Med. 1968 May 1;127(5):967–982. doi: 10.1084/jem.127.5.967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gewurz H., Page A. R., Pickering R. J., Good R. A. Complement activity and inflammatory neutrophil exudation in man. Studies in patients with glomerulonephritis, essential hypocomplementemia and agammaglobulinemia. Int Arch Allergy Appl Immunol. 1967;32(1):64–90. doi: 10.1159/000229917. [DOI] [PubMed] [Google Scholar]
- Ishizaka T., Tada T., Ishizaka K. Fixation of C' and C'la by rabbit gamma-G- and gamma-M-antibodies with particulate and soluble antigens. J Immunol. 1968 Jun;100(6):1145–1153. [PubMed] [Google Scholar]
- Müllerèberhard H. J., Dalmasso A. P., Calcott M. A. The reaction mechanism of beta-1C-globulin (C'3) in immune hemolysis. J Exp Med. 1966 Jan 1;123(1):33–54. doi: 10.1084/jem.123.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NISHIOKA K., LINSCOTT W. D. COMPONENTS OF GUINEA PIG COMPLEMENT. I. SEPARATION OF A SERUM FRACTION ESSENTIAL FOR IMMUNE HEMOLYSIS AND IMMUNE ADHERENCE. J Exp Med. 1963 Nov 1;118:767–793. doi: 10.1084/jem.118.5.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson R. A., Jr, Jensen J., Gigli I., Tamura N. Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum. Immunochemistry. 1966 Mar;3(2):111–135. doi: 10.1016/0019-2791(66)90292-8. [DOI] [PubMed] [Google Scholar]
- Rommel F. A., Stolfi R. Preparation and partial characterization of sheep erythrocyte-antibody-complement intermediate. Immunology. 1968 Oct;15(4):469–479. [PMC free article] [PubMed] [Google Scholar]
- Shin H. S., Mayer M. M. The third component of the guinea pig complement system. II. Kinetic study of the reaction of EAC'4,2a with guinea pig C'3. Enzymatic nature of C'3 comsumption, multiphasic character of fixation, and hemolytic titration of C'3. Biochemistry. 1968 Aug;7(8):2997–3002. doi: 10.1021/bi00848a041. [DOI] [PubMed] [Google Scholar]
- Spooner R. L., Sell K. W. Comparison of the behaviour of IgG and IgM anti-Forssman antibodies in agglutination, haemolysis and cytolysis. Immunology. 1966 Dec;11(6):521–531. [PMC free article] [PubMed] [Google Scholar]
- Tamura N., Nelson R. A., Jr Three naturally-occurring inhibitors of components of complement in guinea pig and rabbit serum. J Immunol. 1967 Sep;99(3):582–589. [PubMed] [Google Scholar]
- Willoughby W. F., Mayer M. M. Antibody-complement complexes. Science. 1965 Nov 12;150(3698):907–908. doi: 10.1126/science.150.3698.907. [DOI] [PubMed] [Google Scholar]