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. 1970 Dec;19(6):901–907.

The total protein and immunoglobulin profile of equine colostrum and milk

B T Rouse, D G Ingram
PMCID: PMC1455671  PMID: 4992198

Abstract

Thirty-six samples of colostrum and milk were collected from ponies at various intervals after parturition. Levels of total protein and immunoglobulins IgG and IgG(T) were determined. In samples collected in the first 3 hours post partum, the total protein concentration was approximately twice that of serum protein and the immunoglobulins IgG and IgG(T) accounted for 30 per cent and 10 per cent respectively of this protein. The ratio of IgG to IgG(T) was similar to that in serum.

After suckling, a marked decline in both total protein and immunoglobulin concentration occurred. In addition, the relative concentration of immunoglobulins declined. Thus, IgG and IgG(T) made up 7 per cent and 2 per cent respectively of the total protein in samples collected 9–24 hours post partum. In all samples examined up to 33 days post partum the concentration of IgG (γ2) was approximately three times the concentration of IgG(T) (γ1). Thus no evidence was found for a selective secretion of a γ1 immunoglobulin in equine colostrum or milk.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asofsky R., Small P. A., Jr Colostral immunoglobulin-A: synthesis in vitro of T-chain by rabbit mammary gland. Science. 1967 Nov 17;158(3803):932–933. doi: 10.1126/science.158.3803.932. [DOI] [PubMed] [Google Scholar]
  2. BLAKEMORE F., GARNER R. J. The maternal transference of antibodies in the bovine. J Comp Pathol. 1956 Oct;66(4):287–289. doi: 10.1016/s0368-1742(56)80030-1. [DOI] [PubMed] [Google Scholar]
  3. Cebra J. J., Robbins J. B. Gamma-A-immunoglobulin from rabbit colostrum. J Immunol. 1966 Jul;97(1):12–24. [PubMed] [Google Scholar]
  4. FEINSTEIN A. CHARACTER AND ALLOTYPY OF AN IMMUNE GLOBULIN IN RABBIT COLOSTRUM. Nature. 1963 Sep 21;199:1197–1199. doi: 10.1038/1991197b0. [DOI] [PubMed] [Google Scholar]
  5. Genco R. J., Yecies L., Karush F. The immunoglobulins of equine colostrum and parotid fluid. J Immunol. 1969 Sep;103(3):437–444. [PubMed] [Google Scholar]
  6. Heimer R., Jones D. W., Maurer P. H. The immunoglobulins of sheep colostrum. Biochemistry. 1969 Oct;8(10):3937–3944. doi: 10.1021/bi00838a009. [DOI] [PubMed] [Google Scholar]
  7. Heremans J. F. Immunoglobulin formation and function in different tissues. Curr Top Microbiol Immunol. 1968;45:131–203. doi: 10.1007/978-3-642-50109-8_4. [DOI] [PubMed] [Google Scholar]
  8. LUNSFORD L., Jr, DEUTSCH H. F. Human milk whey proteins. Proc Soc Exp Biol Med. 1957 Dec;96(3):742–744. doi: 10.3181/00379727-96-23595. [DOI] [PubMed] [Google Scholar]
  9. MURPHY F. A., AALUND O., OSEBOLD J. W., CARROLL E. J. GAMMA GLOBULINS OF BOVINE LACTEAL SECRETIONS. Arch Biochem Biophys. 1964 Nov;108:230–239. doi: 10.1016/0003-9861(64)90380-7. [DOI] [PubMed] [Google Scholar]
  10. Mach J. P., Pahud J. J., Isliker H. IgA with "secretory piece" in bovine colostrum and saliva. Nature. 1969 Aug 30;223(5209):952–955. doi: 10.1038/223952a0. [DOI] [PubMed] [Google Scholar]
  11. NORDBRING F. The change in total nitrogen and electrophoretic pattern in human colostrum during the first days of lactation. Acta Soc Med Ups. 1957;62(3-4):113–129. [PubMed] [Google Scholar]
  12. NORDBRING F. The change in total nitrogen, electrophoretic pattern and antibody titre in porcine and bovine colostrum during the first days of lactation. Acta Soc Med Ups. 1957;62(5-6):135–151. [PubMed] [Google Scholar]
  13. PIERCE A. E., FEINSTEIN A. BIOPHYSICAL AND IMMUNOLOGICAL STUDIES ON BOVINE IMMUNE GLOBULINS WITH EVIDENCE FOR SELECTIVE TRANSPORT WITHIN THE MAMMARY GLAND FROM MATERNAL PLASMA TO COLOSTRUM. Immunology. 1965 Jan;8:106–123. [PMC free article] [PubMed] [Google Scholar]
  14. Porter P., Noakes D. E. Immunoglobulins in sow mammary secretions throughout lactation and their significance as anti-(Escherichia coli) antibodies to the young pig. Biochem J. 1969 Jun;113(2):6P–7P. doi: 10.1042/bj1130006pb. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rockey J. H. Equine antihapten antibody. The subunits and fragments of anti-beta-lactoside antibody. J Exp Med. 1967 Feb 1;125(2):249–275. doi: 10.1084/jem.125.2.249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  17. Sullivan A. L., Prendergast R. A., Antunes L. J., Silverstein A. M., Tomasi T. B., Jr Characterization of the serum and secretory immune systems of the cow and sheep. J Immunol. 1969 Aug;103(2):334–344. [PubMed] [Google Scholar]
  18. Tomasi T. B., Jr, Bienenstock J. Secretory immunoglobulins. Adv Immunol. 1968;9:1–96. doi: 10.1016/s0065-2776(08)60441-1. [DOI] [PubMed] [Google Scholar]
  19. Vaerman J. P., Heremans J. F. The immunoglobulins of the dog. II. The immunoglobulins of canine secretions. Immunochemistry. 1969 Nov;6(6):779–786. doi: 10.1016/0019-2791(69)90284-5. [DOI] [PubMed] [Google Scholar]
  20. Zolla S., Goodman J. W. An aggregating immunoglobulin in hyperimmune equine anti-pneumococcal sera. J Immunol. 1968 Apr;100(4):880–897. [PubMed] [Google Scholar]

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